Lymphoid origin of intrinsically activated plasmacytoid dendritic cells in mice

IF 6.4 1区生物学 Q1 BIOLOGY eLife Pub Date : 2024-09-13 DOI:https://doi.org/10.7554/elife.96394.3

Alessandra Machado Araujo, Joseph D Dekker, Kendra Garrison, Zhe Su, Catherine Rhee, Zicheng Hu, Bum-Kyu Lee, Daniel Osorio, Jiwon Lee, Vishwanath R Iyer, Lauren IR Ehrlich, George Georgiou, Gregory Ippolito, Stephen Yi, Haley O Tucker

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Abstract

We identified a novel mouse plasmacytoid dendritic cell (pDC) lineage derived from the common lymphoid progenitors (CLPs) that is dependent on expression of Bcl11a. These CLP-derived pDCs, which we refer to as ‘B-pDCs’, have a unique gene expression profile that includes hallmark B cell genes, normally not expressed in conventional pDCs. Despite expressing most classical pDC markers such as SIGLEC-H and PDCA1, B-pDCs lack IFN-α secretion, exhibiting a distinct inflammatory profile. Functionally, B-pDCs induce T cell proliferation more robustly than canonical pDCs following Toll-like receptor 9 (TLR9) engagement. B-pDCs, along with another homogeneous subpopulation of myeloid-derived pDCs, display elevated levels of the cell surface receptor tyrosine kinase AXL, mirroring human AXL⁺ transitional DCs in function and transcriptional profile. Murine B-pDCs therefore represent a phenotypically and functionally distinct CLP-derived DC lineage specialized in T cell activation and previously not described in mice.

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小鼠固有活化浆细胞树突状细胞的淋巴来源

我们发现了一种新型小鼠浆细胞树突状细胞（pDC）系，这种细胞系来源于普通淋巴祖细胞（CLPs），依赖于 Bcl11a 的表达。这些由 CLP 衍生的 pDC（我们称之为 "B-pDC"）具有独特的基因表达谱，其中包括 B 细胞标志基因，这些基因通常不在传统 pDC 中表达。尽管 B-pDCs 表达大多数经典的 pDC 标记，如 SIGLEC-H 和 PDCA1，但它们缺乏 IFN-α 分泌，表现出独特的炎症特征。在功能上，B-pDC 与典型 pDC 相比，在 Toll 样受体 9（TLR9）参与后能更强有力地诱导 T 细胞增殖。B-pDCs与另一个同质的髓源性pDCs亚群一起显示出细胞表面受体酪氨酸激酶AXL水平的升高，在功能和转录特征上与人类AXL+过渡性DCs相似。因此，小鼠 B-pDCs 代表了一种表型和功能上与众不同的 CLP 派生 DC 系，专门用于 T 细胞活化，以前未在小鼠中描述过。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

eLife BIOLOGY-

CiteScore

12.90

自引率

3.90%

发文量

3122

审稿时长

17 weeks

期刊介绍： eLife is a distinguished, not-for-profit, peer-reviewed open access scientific journal that specializes in the fields of biomedical and life sciences. eLife is known for its selective publication process, which includes a variety of article types such as: Research Articles: Detailed reports of original research findings. Short Reports: Concise presentations of significant findings that do not warrant a full-length research article. Tools and Resources: Descriptions of new tools, technologies, or resources that facilitate scientific research. Research Advances: Brief reports on significant scientific advancements that have immediate implications for the field. Scientific Correspondence: Short communications that comment on or provide additional information related to published articles. Review Articles: Comprehensive overviews of a specific topic or field within the life sciences.