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Host-derived Lactobacillus plantarum alleviates hyperuricemia by improving gut microbial community and hydrolase-mediated degradation of purine nucleosides. 宿主源植物乳杆菌通过改善肠道微生物群落和水解酶介导的嘌呤核苷降解,缓解高尿酸血症。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-07 DOI: 10.7554/eLife.100068
Yang Fu, Xiao-Dan Luo, Jin-Ze Li, Qian-Yuan Mo, Xue Wang, Yue Zhao, You-Ming Zhang, Hao-Tong Luo, Dai-Yang Xia, Wei-Qing Ma, Jian-Ying Chen, Li-Hau Wang, Qiu-Yi Deng, Lukuyu Ben, Muhammad Kashif Saleemi, Xian-Zhi Jiang, Juan Chen, Kai Miao, Zhen-Ping Lin, Peng Zhang, Hui Ye, Qing-Yun Cao, Yong-Wen Zhu, Lin Yang, Qiang Tu, Wence Wang

The gut microbiota is implicated in the pathogenesis of hyperuricemia (HUA) and gout. However, it remains unclear whether probiotics residing in the host gut, such as Lactobacillus, can prevent HUA development. Herein, we isolated Lactobacillus plantarum SQ001 from the cecum of HUA geese and conducted in vitro assays on uric acid (UA) and nucleoside co-culture. Metabolomics and genome-wide analyses, revealed that this strain may promote nucleoside uptake and hydrolysis through its nucleoside hydrolase gene. The functional role of iunH gene was confirmed via heterologous expression and gene knockout studies. Oral administration of L. plantarum SQ001 resulted in increased abundance of Lactobacillus species and reduced serum UA levels. Furthermore, it downregulated hepatic xanthine oxidase, a key enzyme involved in UA synthesis, as well as renal reabsorption protein GLUT9, while enhancing the expression of renal excretion protein ABCG2. Our findings suggest that L. plantarum has potential to ameliorate gut microbial dysbiosis with HUA, thereby offering insights into its potential application as a probiotic therapy for individuals with HUA or gout.

肠道微生物群与高尿酸血症(HUA)和痛风的发病机制有关。然而,寄居在宿主肠道中的益生菌(如乳酸杆菌)是否能预防 HUA 的发生仍不清楚。在此,我们从 HUA 鹅的盲肠中分离出植物乳杆菌 SQ001,并进行了尿酸(UA)和核苷共培养的体外试验。代谢组学和全基因组分析表明,该菌株可能通过其核苷水解酶基因促进核苷的吸收和水解。通过异源表达和基因敲除研究证实了 iunH 基因的功能作用。口服植物乳杆菌 SQ001 增加了乳酸杆菌的数量,降低了血清 UA 水平。此外,植物乳杆菌 SQ001 还能下调肝脏黄嘌呤氧化酶(参与尿酸合成的关键酶)和肾脏重吸收蛋白 GLUT9,同时增强肾脏排泄蛋白 ABCG2 的表达。我们的研究结果表明,植物乳杆菌具有改善HUA肠道微生物菌群失调的潜力,从而为其作为一种益生菌疗法应用于HUA或痛风患者提供了启示。
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引用次数: 0
Single-cell multiomics analysis of chronic myeloid leukemia links cellular heterogeneity to therapy response. 慢性髓性白血病的单细胞多组学分析将细胞异质性与治疗反应联系起来。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-06 DOI: 10.7554/eLife.92074
Rebecca Warfvinge, Linda Geironson Ulfsson, Parashar Dhapola, Fatemeh Safi, Mikael Sommarin, Shamit Soneji, Henrik Hjorth-Hansen, Satu Mustjoki, Johan Richter, Ram Krishna Thakur, Göran Karlsson

The advent of tyrosine kinase inhibitors (TKIs) as treatment of chronic myeloid leukemia (CML) is a paradigm in molecularly targeted cancer therapy. Nonetheless, TKI-insensitive leukemia stem cells (LSCs) persist in most patients even after years of treatment and are imperative for disease progression as well as recurrence during treatment-free remission (TFR). Here, we have generated high-resolution single-cell multiomics maps from CML patients at diagnosis, retrospectively stratified by BCR::ABL1IS (%) following 12 months of TKI therapy. Simultaneous measurement of global gene expression profiles together with >40 surface markers from the same cells revealed that each patient harbored a unique composition of stem and progenitor cells at diagnosis. The patients with treatment failure after 12 months of therapy had a markedly higher abundance of molecularly defined primitive cells at diagnosis compared to the optimal responders. The multiomic feature landscape enabled visualization of the primitive fraction as a mixture of molecularly distinct BCR::ABL1+ LSCs and BCR::ABL1-hematopoietic stem cells (HSCs) in variable ratio across patients, and guided their prospective isolation by a combination of CD26 and CD35 cell surface markers. We for the first time show that BCR::ABL1+ LSCs and BCR::ABL1- HSCs can be distinctly separated as CD26+CD35- and CD26-CD35+, respectively. In addition, we found the ratio of LSC/HSC to be higher in patients with prospective treatment failure compared to optimal responders, at diagnosis as well as following 3 months of TKI therapy. Collectively, this data builds a framework for understanding therapy response and adapting treatment by devising strategies to extinguish or suppress TKI-insensitive LSCs.

酪氨酸激酶抑制剂(TKIs)治疗慢性髓性白血病(CML)的出现是分子靶向癌症治疗的典范。然而,对TKI不敏感的白血病干细胞(LSCs)即使在经过多年治疗后仍然存在于大多数患者体内,并且是疾病进展以及无治疗缓解期(TFR)复发的必要条件。在这里,我们生成了CML患者诊断时的高分辨率单细胞多组学图谱,并在TKI治疗12个月后按BCR::ABL1IS(%)进行了回顾性分层。同时测量的全基因表达谱和来自相同细胞的40多种表面标记物显示,每位患者在诊断时都拥有独特的干细胞和祖细胞组成。与最佳应答者相比,治疗12个月后治疗失败的患者在诊断时分子定义的原始细胞丰度明显更高。多基因组特征图谱使原始细胞部分可视化为分子上不同的BCR::ABL1+ LSCs和BCR::ABL1-造血干细胞(HSCs)的混合物,它们在不同患者中的比例各不相同,并通过CD26和CD35细胞表面标志物的组合指导它们的前瞻性分离。我们首次发现,BCR::ABL1+造血干细胞和BCR::ABL1-造血干细胞可分别以CD26+CD35-和CD26-CD35+的形式区分开来。此外,我们还发现,在诊断时以及接受 TKI 治疗 3 个月后,与最佳应答者相比,前瞻性治疗失败患者的 LSC/HSC 比例更高。总之,这些数据为了解治疗反应和通过制定策略消灭或抑制对 TKI 不敏感的 LSCs 来调整治疗建立了一个框架。
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引用次数: 0
DNAH3 deficiency causes flagellar inner dynein arm loss and male infertility in humans and mice. DNAH3 缺乏会导致鞭毛内动力蛋白臂缺失以及人类和小鼠雄性不育。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-06 DOI: 10.7554/eLife.96755
Xiang Wang, Gan Shen, Yihong Yang, Chuan Jiang, Tiechao Ruan, Xue Yang, Liangchai Zhuo, Yingteng Zhang, Yangdi Ou, Xinya Zhao, Shunhua Long, Xiangrong Tang, Tingting Lin, Ying Shen

Axonemal protein complexes, including the outer and inner dynein arms (ODA/IDA), are highly ordered structures of the sperm flagella that drive sperm motility. Deficiencies in several axonemal proteins have been associated with male infertility, which is characterized by asthenozoospermia or asthenoteratozoospermia. Dynein axonemal heavy chain 3 (DNAH3) resides in the IDA and is highly expressed in the testis. However, the relationship between DNAH3 and male infertility is still unclear. Herein, we identified biallelic variants of DNAH3 in four unrelated Han Chinese infertile men with asthenoteratozoospermia through whole-exome sequencing (WES). These variants contributed to deficient DNAH3 expression in the patients' sperm flagella. Importantly, the patients represented the anomalous sperm flagellar morphology, and the flagellar ultrastructure was severely disrupted. Intriguingly, Dnah3 knockout (KO) male mice were also infertile, especially showing the severe reduction in sperm movement with the abnormal IDA and mitochondrion structure. Mechanically, nonfunctional DNAH3 expression resulted in decreased expression of IDA-associated proteins in the spermatozoa flagella of patients and KO mice, including DNAH1, DNAH6, and DNALI1, the deletion of which has been involved in disruption of sperm motility. Moreover, the infertility of patients with DNAH3 variants and Dnah3 KO mice could be rescued by intracytoplasmic sperm injection (ICSI) treatment. Our findings indicated that DNAH3 is a novel pathogenic gene for asthenoteratozoospermia and may further contribute to the diagnosis, genetic counseling, and prognosis of male infertility.

轴丝蛋白复合物(包括外侧和内侧动力蛋白臂(ODA/IDA))是精子鞭毛的高度有序结构,可驱动精子运动。几种轴突蛋白的缺乏与男性不育有关,表现为无精子症或少精子症。Dynein轴突蛋白重链3(DNAH3)位于IDA中,在睾丸中高度表达。然而,DNAH3与男性不育之间的关系仍不清楚。在此,我们通过全外显子组测序(WES)在四名患有无精子症的无血缘关系汉族不育男性中发现了DNAH3的双拷贝变体。这些变异导致患者精子鞭毛中DNAH3表达不足。重要的是,这些患者的精子鞭毛形态异常,鞭毛超微结构受到严重破坏。耐人寻味的是,Dnah3基因敲除(KO)雄性小鼠也不育,尤其是精子运动能力严重下降,IDA和线粒体结构异常。从机理上讲,DNAH3的无功能表达导致患者和KO小鼠精子鞭毛中IDA相关蛋白的表达减少,包括DNAH1、DNAH6和DNALI1,这些蛋白的缺失参与了精子活力的破坏。此外,DNAH3变体患者和Dnah3 KO小鼠的不育症可以通过卵胞浆内单精子注射(ICSI)治疗得到挽救。我们的研究结果表明,DNAH3是无精子症的一个新的致病基因,可进一步促进男性不育症的诊断、遗传咨询和预后。
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引用次数: 0
Distinct catecholaminergic pathways projecting to hippocampal CA1 transmit contrasting signals during navigation in familiar and novel environments. 在熟悉和陌生环境中导航时,投射到海马CA1的不同儿茶酚胺能通路传递的信号截然不同。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-06 DOI: 10.7554/eLife.95213
Chad Heer, Mark Sheffield

Neuromodulatory inputs to the hippocampus play pivotal roles in modulating synaptic plasticity, shaping neuronal activity, and influencing learning and memory. Recently, it has been shown that the main sources of catecholamines to the hippocampus, ventral tegmental area (VTA) and locus coeruleus (LC), may have overlapping release of neurotransmitters and effects on the hippocampus. Therefore, to dissect the impacts of both VTA and LC circuits on hippocampal function, a thorough examination of how these pathways might differentially operate during behavior and learning is necessary. We therefore utilized two-photon microscopy to functionally image the activity of VTA and LC axons within the CA1 region of the dorsal hippocampus in head-fixed male mice navigating linear paths within virtual reality (VR) environments. We found that within familiar environments some VTA axons and the vast majority of LC axons showed a correlation with the animals' running speed. However, as mice approached previously learned rewarded locations, a large majority of VTA axons exhibited a gradual ramping-up of activity, peaking at the reward location. In contrast, LC axons displayed a pre-movement signal predictive of the animal's transition from immobility to movement. Interestingly, a marked divergence emerged following a switch from the familiar to novel VR environments. Many LC axons showed large increases in activity that remained elevated for over a minute, while the previously observed VTA axon ramping-to-reward dynamics disappeared during the same period. In conclusion, these findings highlight distinct roles of VTA and LC catecholaminergic inputs in the dorsal CA1 hippocampal region. These inputs encode unique information, with reward information in VTA inputs and novelty and kinematic information in LC inputs, likely contributing to differential modulation of hippocampal activity during behavior and learning.

海马的神经调节输入在调节突触可塑性、塑造神经元活动以及影响学习和记忆方面发挥着关键作用。最近的研究表明,儿茶酚胺对海马的主要来源--腹侧被盖区(VTA)和室上皮层(LC)--可能有重叠的神经递质释放和对海马的影响。因此,要剖析 VTA 和 LC 环路对海马功能的影响,就必须彻底研究这些通路如何在行为和学习过程中以不同方式运行。因此,我们利用双光子显微镜对头固定的雄性小鼠在虚拟现实(VR)环境中通过线性路径导航时海马背侧CA1区内的VTA和LC轴突活动进行了功能成像。我们发现,在熟悉的环境中,部分VTA轴突和绝大多数LC轴突与动物的奔跑速度相关。然而,当小鼠接近先前学习过的奖励位置时,绝大多数 VTA 轴突的活动逐渐增强,并在奖励位置达到峰值。与此相反,LC 轴突显示出运动前信号,预测动物从静止到运动的过渡。有趣的是,从熟悉的 VR 环境切换到新奇的 VR 环境后,出现了明显的分化。许多LC轴突的活动出现大幅增加,并在超过一分钟的时间内保持高水平,而之前观察到的VTA轴突从上升到奖励的动态在同一时期消失了。总之,这些发现突显了 VTA 和 LC 儿茶酚胺能输入在背侧 CA1 海马区的不同作用。这些输入编码了独特的信息,VTA 输入编码奖励信息,LC 输入编码新奇和运动信息,可能有助于在行为和学习过程中对海马活动进行不同的调节。
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引用次数: 0
Functional implications of the exon 9 splice insert in GluK1 kainate receptors. GluK1 kainate 受体第 9 号外显子剪接插入的功能影响。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-06 DOI: 10.7554/eLife.89755
Surbhi Dhingra, Prachi M Chopade, Rajesh Vinnakota, Janesh Kumar

Kainate receptors are key modulators of synaptic transmission and plasticity in the central nervous system. Different kainate receptor isoforms with distinct spatiotemporal expressions have been identified in the brain. The GluK1-1 splice variant receptors, which are abundant in the adult brain, have an extra fifteen amino acids inserted in the amino-terminal domain (ATD) of the receptor resulting from alternative splicing of exon 9. However, the functional implications of this post-transcriptional modification are not yet clear. We employed a multi-pronged approach using cryogenic electron microscopy, electrophysiology, and other biophysical and biochemical tools to understand the structural and functional impact of this splice insert in the extracellular domain of GluK1 receptors. Our study reveals that the splice insert alters the key gating properties of GluK1 receptors and their modulation by the cognate auxiliary Neuropilin and tolloid-like (Neto) proteins 1 and 2. Mutational analysis identified the role of crucial splice residues that influence receptor properties and their modulation. Furthermore, the cryoEM structure of the variant shows that the presence of exon 9 in GluK1 does not affect the receptor architecture or domain arrangement in the desensitized state. Our study thus provides the first detailed structural and functional characterization of GluK1-1a receptors, highlighting the role of the splice insert in modulating receptor properties and their modulation.

凯恩酸盐受体是中枢神经系统突触传递和可塑性的关键调节剂。在大脑中已发现不同的凯恩酸受体异构体,它们具有不同的时空表达。在成人大脑中大量存在的 GluK1-1 剪接变体受体,由于外显子 9 的替代剪接,在受体的氨基末端结构域(ATD)中多插入了 15 个氨基酸。然而,这种转录后修饰的功能影响尚不清楚。我们采用低温电子显微镜、电生理学以及其他生物物理和生物化学工具多管齐下的方法来了解 GluK1 受体胞外结构域中这种剪接插入的结构和功能影响。我们的研究发现,该剪接插入物改变了 GluK1 受体的关键门控特性,以及同源辅助神经蛋白和类拓扑(Neto)蛋白 1 和 2 对这些特性的调节作用。突变分析确定了影响受体特性及其调控的关键剪接残基的作用。此外,变体的冷冻电镜结构显示,GluK1 中外显子 9 的存在并不影响脱敏状态下的受体结构或结构域排列。因此,我们的研究首次提供了 GluK1-1a 受体的详细结构和功能特征,突出了剪接插入物在调节受体特性及其调制过程中的作用。
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引用次数: 0
Predicting drug resistance. 预测耐药性。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-06 DOI: 10.7554/eLife.103775
Nicole S Arellano, Shannon E Elf

A new approach helps examine the proportion of cancerous and healthy stem cells in patients with chronic myeloid leukemia and how this influences treatment outcomes.

一种新方法有助于研究慢性骨髓性白血病患者体内癌干细胞和健康干细胞的比例,以及这对治疗效果的影响。
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引用次数: 0
Natural forgetting reversibly modulates engram expression. 自然遗忘可逆地调节刻痕表达。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-05 DOI: 10.7554/eLife.92860
James D O'Leary, Rasmus Bruckner, Livia Autore, Tomás J Ryan

Memories are stored as ensembles of engram neurons and their successful recall involves the reactivation of these cellular networks. However, significant gaps remain in connecting these cell ensembles with the process of forgetting. Here, we utilized a mouse model of object memory and investigated the conditions in which a memory could be preserved, retrieved, or forgotten. Direct modulation of engram activity via optogenetic stimulation or inhibition either facilitated or prevented the recall of an object memory. In addition, through behavioral and pharmacological interventions, we successfully prevented or accelerated forgetting of an object memory. Finally, we showed that these results can be explained by a computational model in which engrams that are subjectively less relevant for adaptive behavior are more likely to be forgotten. Together, these findings suggest that forgetting may be an adaptive form of engram plasticity which allows engrams to switch from an accessible state to an inaccessible state.

记忆是以刻画神经元组合的形式存储的,要成功唤起记忆,就必须重新激活这些细胞网络。然而,在将这些细胞集合与遗忘过程联系起来方面仍存在巨大差距。在这里,我们利用小鼠的物体记忆模型,研究了记忆得以保存、检索或遗忘的条件。通过光遗传学刺激或抑制直接调节刻痕活动可以促进或阻止物体记忆的回忆。此外,通过行为和药物干预,我们成功地防止或加速了物体记忆的遗忘。最后,我们证明这些结果可以用一个计算模型来解释,在这个模型中,主观上与适应行为相关性较低的记忆点更有可能被遗忘。这些发现共同表明,遗忘可能是一种适应性的刻痕可塑性形式,它允许刻痕从可访问状态切换到不可访问状态。
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引用次数: 0
The recurrent temporal restricted Boltzmann machine captures neural assembly dynamics in whole-brain activity. 递归时间限制波尔兹曼机捕捉全脑活动中的神经装配动态。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-05 DOI: 10.7554/eLife.98489
Sebastian Quiroz Monnens, Casper Peters, Luuk Willem Hesselink, Kasper Smeets, Bernhard Englitz

Animal behaviour alternates between stochastic exploration and goal-directed actions, which are generated by the underlying neural dynamics. Previously, we demonstrated that the compositional Restricted Boltzmann Machine (cRBM) can decompose whole-brain activity of larval zebrafish data at the neural level into a small number (∼100-200) of assemblies that can account for the stochasticity of the neural activity (van der Plas et al., eLife, 2023). Here, we advance this representation by extending to a combined stochastic-dynamical representation to account for both aspects using the recurrent temporal RBM (RTRBM) and transfer-learning based on the cRBM estimate. We demonstrate that the functional advantage of the RTRBM is captured in the temporal weights on the hidden units, representing neural assemblies, for both simulated and experimental data. Our results show that the temporal expansion outperforms the stochastic-only cRBM in terms of generalization error and achieves a more accurate representation of the moments in time. Lastly, we demonstrate that we can identify the original time-scale of assembly dynamics by estimating multiple RTRBMs at different temporal resolutions. Together, we propose that RTRBMs are a valuable tool for capturing the combined stochastic and time-predictive dynamics of large-scale data sets.

动物行为在随机探索和目标行动之间交替进行,而目标行动是由潜在的神经动力学产生的。在此之前,我们已经证明,构图受限玻尔兹曼机(cRBM)可以在神经水平上将幼体斑马鱼数据的全脑活动分解为少量(100-200)可解释神经活动随机性的集合体(van der Plas 等人,eLife,2023)。在这里,我们将这一表征扩展为随机-动态组合表征,利用递归时序 RBM(RTRBM)和基于 cRBM 估计的迁移学习来考虑这两个方面。我们通过模拟和实验数据证明,RTRBM 的功能优势体现在代表神经集合的隐藏单元的时间权重上。我们的结果表明,就泛化误差而言,时间扩展优于纯随机 cRBM,并能更准确地表示时间时刻。最后,我们证明了可以通过在不同时间分辨率下估计多个 RTRBM 来识别装配动态的原始时间尺度。综上所述,我们认为 RTRBM 是捕捉大规模数据集的随机和时间预测动态的重要工具。
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引用次数: 0
Predictably manipulating photoreceptor light responses to reveal their role in downstream visual responses. 可预测地操纵感光器的光反应,揭示它们在下游视觉反应中的作用。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-05 DOI: 10.7554/eLife.93795
Qiang Chen, Norianne T Ingram, Jacob Baudin, Juan M Angueyra, Raunak Sinha, Fred Rieke

Computation in neural circuits relies on the judicious use of nonlinear circuit components. In many cases, multiple nonlinear components work collectively to control circuit outputs. Separating the contributions of these different components is difficult, and this limits our understanding of the mechanistic basis of many important computations. Here, we introduce a tool that permits the design of light stimuli that predictably alter rod and cone phototransduction currents - including stimuli that compensate for nonlinear properties such as light adaptation. This tool, based on well-established models for the rod and cone phototransduction cascade, permits the separation of nonlinearities in phototransduction from those in downstream circuits. This will allow, for example, direct tests of how adaptation in rod and cone phototransduction affects downstream visual signals and perception.

神经回路中的计算依赖于对非线性电路元件的合理使用。在许多情况下,多个非线性元件共同控制电路输出。将这些不同元件的贡献分离开来非常困难,这限制了我们对许多重要计算的机理基础的理解。在这里,我们介绍了一种工具,它允许设计可预测改变杆状和锥状光传导电流的光刺激,包括补偿非线性特性(如光适应)的刺激。该工具基于成熟的视杆细胞和视锥细胞光传导级联模型,可将光传导中的非线性与下游电路中的非线性分离开来。例如,这样就可以直接测试视杆和视锥光传导中的适应性如何影响下游视觉信号和感知。
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引用次数: 0
SntB triggers the antioxidant pathways to regulate development and aflatoxin biosynthesis in Aspergillus flavus. SntB 触发抗氧化途径,调节黄曲霉的发育和黄曲霉毒素的生物合成。
IF 6.4 1区 生物学 Q1 BIOLOGY Pub Date : 2024-11-05 DOI: 10.7554/eLife.94743
Dandan Wu, Chi Yang, Yanfang Yao, Dongmei Ma, Hong Lin, Ling Hao, Wenwen Xin, Kangfu Ye, Minghui Sun, Yule Hu, Yanling Yang, Zhenhong Zhuang

The epigenetic reader SntB was identified as an important transcriptional regulator of growth, development, and secondary metabolite synthesis in Aspergillus flavus. However, the underlying molecular mechanism is still unclear. In this study, by gene deletion and complementation, we found SntB is essential for mycelia growth, conidial production, sclerotia formation, aflatoxin synthesis, and host colonization. Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq) analysis revealed that SntB played key roles in oxidative stress response of A. flavus, influencing related gene activity, especially catC encoding catalase. SntB regulated the expression activity of catC with or without oxidative stress, and was related to the expression level of the secretory lipase (G4B84_008359). The deletion of catC showed that CatC participated in the regulation of fungal morphogenesis, reactive oxygen species (ROS) level, and aflatoxin production, and that CatC significantly regulated fungal sensitive reaction and AFB1 yield under oxidative stress. Our study revealed the potential machinery that SntB regulated fungal morphogenesis, mycotoxin anabolism, and fungal virulence through the axle of from H3K36me3 modification to fungal virulence and mycotoxin biosynthesis. The results of this study shed light into the SntB-mediated transcript regulation pathways of fungal mycotoxin anabolism and virulence, which provided potential strategy to control the contamination of A. flavus and its aflatoxins.

表观遗传阅读器 SntB 被确定为黄曲霉生长、发育和次生代谢物合成的重要转录调节因子。然而,其潜在的分子机制仍不清楚。本研究通过基因缺失和互补,发现 SntB 对菌丝生长、分生孢子产生、硬菌丝形成、黄曲霉毒素合成和宿主定殖至关重要。染色质免疫沉淀测序(ChIP-seq)和RNA测序(RNA-seq)分析表明,SntB在黄曲霉的氧化应激反应中起关键作用,影响相关基因的活性,尤其是编码过氧化氢酶的catC。无论是否存在氧化应激,SntB 都能调控 catC 的表达活性,并与分泌脂肪酶(G4B84_008359)的表达水平相关。缺失 catC 表明,CatC 参与了真菌形态发生、活性氧(ROS)水平和黄曲霉毒素产量的调控,并且 CatC 显著调控了氧化胁迫下真菌的敏感反应和 AFB1 产量。我们的研究揭示了SntB通过从H3K36me3修饰到真菌毒力和真菌毒素生物合成的轴线调控真菌形态发生、真菌毒素合成代谢和真菌毒力的潜在机制。研究结果揭示了SntB介导的真菌霉菌毒素合成代谢和毒力的转录调控途径,为控制黄曲霉及其黄曲霉毒素的污染提供了潜在的策略。
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引用次数: 0
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