Computational analysis and expression profiling of NAC transcription factor family involved in biotic stress response in Manihot esculenta

IF 4.2 3区 生物学 Q1 PLANT SCIENCES Plant Biology Pub Date : 2024-09-12 DOI:10.1111/plb.13715
A. H. Abdoulaye, C. Yuhua, Z. Xiaoyan, Y. Yiwei, H. Wang, C. Yinhua
{"title":"Computational analysis and expression profiling of NAC transcription factor family involved in biotic stress response in Manihot esculenta","authors":"A. H. Abdoulaye, C. Yuhua, Z. Xiaoyan, Y. Yiwei, H. Wang, C. Yinhua","doi":"10.1111/plb.13715","DOIUrl":null,"url":null,"abstract":"<jats:list list-type=\"bullet\"> <jats:list-item>The Nascent polypeptide‐Associated Complex (NAC) family is among the largest plant‐specific TF families and plays an important role in plant growth, development, and stress responses.</jats:list-item> <jats:list-item>NAC TFs have been extensively studied in plants such as rice and <jats:italic>Arabidopsis</jats:italic>; however, their characterization, functions, evolution, and expression patterns in <jats:italic>Manihot esculenta</jats:italic> (cassava) under environmental stress remain largely unexplored. Here, we used bioinformatic analyses and biotic stress responses to investigate the physicochemical properties, chromosome location, phylogeny, gene structure, expression patterns, and <jats:italic>cis</jats:italic>‐elements in promoter regions of the NAC TFs in cassava.</jats:list-item> <jats:list-item>We identified 119 <jats:italic>M. esculenta</jats:italic> NAC (MeNAC) gene families, unevenly distributed on 16 chromosomes. We investigated expression patterns of all identified MeNAC TFs under <jats:italic>Xanthomonas axonopodis</jats:italic> pv. <jats:italic>manihotis</jats:italic> (<jats:italic>Xam</jats:italic>) infection, strain CHN11, at different time points. Only 20 MeNAC TFs showed expression of significant bacterial resistance. Six MeNACs (MeNAC7, 26, 63, 65, 77, and 113) were selected for functional analysis. qRT‐PCR assays revealed that MeNAC7, 26, 63, 65, 77, and 113 were induced in response to <jats:italic>Xam</jats:italic>CHN11 infection and may participate in the molecular interaction of cassava and bacterial blight. Interestingly, MeNAC26, MeNAC63, MeNAC65, and MeNAC113 responded to <jats:italic>Xam</jats:italic>CHN11 infection at 3 h post‐inoculation. Furthermore, we identified 13 stress‐related <jats:italic>cis</jats:italic>‐elements in promoter regions of the MeNAC genes that are involved in diverse environmental stress responses. Phylogenetic analysis revealed that MeNAC genes with similar structures and motif distributions were grouped.</jats:list-item> <jats:list-item>This study provides valuable insights into the evolution, diversity, and characterization of MeNAC TFs. It lays the groundwork for a better understanding of their biological roles and molecular mechanisms in cassava.</jats:list-item> </jats:list>","PeriodicalId":220,"journal":{"name":"Plant Biology","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/plb.13715","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

The Nascent polypeptide‐Associated Complex (NAC) family is among the largest plant‐specific TF families and plays an important role in plant growth, development, and stress responses. NAC TFs have been extensively studied in plants such as rice and Arabidopsis; however, their characterization, functions, evolution, and expression patterns in Manihot esculenta (cassava) under environmental stress remain largely unexplored. Here, we used bioinformatic analyses and biotic stress responses to investigate the physicochemical properties, chromosome location, phylogeny, gene structure, expression patterns, and cis‐elements in promoter regions of the NAC TFs in cassava. We identified 119 M. esculenta NAC (MeNAC) gene families, unevenly distributed on 16 chromosomes. We investigated expression patterns of all identified MeNAC TFs under Xanthomonas axonopodis pv. manihotis (Xam) infection, strain CHN11, at different time points. Only 20 MeNAC TFs showed expression of significant bacterial resistance. Six MeNACs (MeNAC7, 26, 63, 65, 77, and 113) were selected for functional analysis. qRT‐PCR assays revealed that MeNAC7, 26, 63, 65, 77, and 113 were induced in response to XamCHN11 infection and may participate in the molecular interaction of cassava and bacterial blight. Interestingly, MeNAC26, MeNAC63, MeNAC65, and MeNAC113 responded to XamCHN11 infection at 3 h post‐inoculation. Furthermore, we identified 13 stress‐related cis‐elements in promoter regions of the MeNAC genes that are involved in diverse environmental stress responses. Phylogenetic analysis revealed that MeNAC genes with similar structures and motif distributions were grouped. This study provides valuable insights into the evolution, diversity, and characterization of MeNAC TFs. It lays the groundwork for a better understanding of their biological roles and molecular mechanisms in cassava.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
参与 Manihot esculenta 生物胁迫响应的 NAC 转录因子家族的计算分析和表达谱分析
新生多肽相关复合物(NAC)家族是最大的植物特异性 TF 家族之一,在植物生长、发育和胁迫响应中发挥着重要作用。NAC TFs 在水稻和拟南芥等植物中得到了广泛的研究;然而,它们在木薯(Manihot esculenta)环境胁迫下的特征、功能、进化和表达模式在很大程度上仍未得到探索。在此,我们利用生物信息学分析和生物胁迫反应研究了木薯中 NAC TFs 的理化性质、染色体位置、系统发育、基因结构、表达模式以及启动子区域的顺式元件。我们发现了 119 个木薯 NAC(MeNAC)基因家族,它们不均匀地分布在 16 条染色体上。我们研究了在黄单胞菌 Xanthomonas axonopodis pv. manihotis(Xam)感染下,菌株 CHN11 在不同时间点的表达模式。只有 20 个 MeNAC TFs 表现出明显的细菌抗性。qRT-PCR 分析显示,MeNAC7、26、63、65、77 和 113 在 XamCHN11 感染后被诱导,可能参与了木薯与细菌性枯萎病的分子相互作用。有趣的是,MeNAC26、MeNAC63、MeNAC65和MeNAC113在接种后3小时就对XamCHN11感染做出了反应。此外,我们还在 MeNAC 基因的启动子区域发现了 13 个与胁迫相关的顺式元件,它们参与了多种环境胁迫反应。系统进化分析表明,具有相似结构和基序分布的 MeNAC 基因被归为一组。这项研究为了解 MeNAC TFs 的进化、多样性和特征提供了宝贵的见解。它为更好地了解它们在木薯中的生物学作用和分子机制奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Plant Biology
Plant Biology 生物-植物科学
CiteScore
8.20
自引率
2.60%
发文量
109
审稿时长
3 months
期刊介绍: Plant Biology is an international journal of broad scope bringing together the different subdisciplines, such as physiology, molecular biology, cell biology, development, genetics, systematics, ecology, evolution, ecophysiology, plant-microbe interactions, and mycology. Plant Biology publishes original problem-oriented full-length research papers, short research papers, and review articles. Discussion of hot topics and provocative opinion articles are published under the heading Acute Views. From a multidisciplinary perspective, Plant Biology will provide a platform for publication, information and debate, encompassing all areas which fall within the scope of plant science.
期刊最新文献
Linking phylogenetic niche conservatism in bacterial communities in sorghum root compartments revealed by the Hongyingzi cultivar. Ethyl methanesulfonate (EMS) mediated dwarfing mutation provides a basis for CaCO3 accumulation by enhancing photosynthetic performance in Ceratostigma willmottianum Stapf. Stilbene production as part of drought adaptation mechanisms in cultivated grapevine (Vitis vinifera L.) roots modulates antioxidant status. Correction to Blue and UV-B light synergistically induce anthocyanin accumulation by co-activating nitrate reductase gene expression in Anthocyanin fruit (Aft) tomato. Metabolite analysis of peach (Prunus persica L. Batsch) branches in response to freezing stress.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1