{"title":"The RNAi lethal effect of a serine protease gene (TcSP3) on Tetranychus cinnabarinus and its safety evaluation","authors":"Xing-Ru Chen, Teng-Yue Rao, Guang-Ming Liu, Yi-Ni Chen, Yan-Jie Luo, Lin He, Guang-Mao Shen","doi":"10.1127/entomologia/2024/2335","DOIUrl":null,"url":null,"abstract":"RNA interference technology has been recognized in recent years as an ideal and specific new approach for pest control. In this study, a serine protease gene TcSP3 was found specifically expressed during the egg stage of Tetranychus cinnabarinus. Treatment of dsTcSP3 caused high mortality in egg and larvae stages of T. cinnabarinus, but a lower mortality rate after treating adult mites. Persistence analysis showed that dsTcSP3 degraded rapidly after application to cowpea leaves and was essentially undetectable after 60 h. It was also safe to Neoseiulus barkeri and caused essentially no death or deformity of N. barkeri. In conclusion, silencing of the serine protease gene TcSP3 successfully impeded the growth and development process of T. cinnabarinus with less impact on the environment and predatory mite (Neoseiulus barkeri), which can be used as a new target of RNAi for T. cinnabarinus.","PeriodicalId":11728,"journal":{"name":"Entomologia Generalis","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Entomologia Generalis","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1127/entomologia/2024/2335","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
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Abstract
RNA interference technology has been recognized in recent years as an ideal and specific new approach for pest control. In this study, a serine protease gene TcSP3 was found specifically expressed during the egg stage of Tetranychus cinnabarinus. Treatment of dsTcSP3 caused high mortality in egg and larvae stages of T. cinnabarinus, but a lower mortality rate after treating adult mites. Persistence analysis showed that dsTcSP3 degraded rapidly after application to cowpea leaves and was essentially undetectable after 60 h. It was also safe to Neoseiulus barkeri and caused essentially no death or deformity of N. barkeri. In conclusion, silencing of the serine protease gene TcSP3 successfully impeded the growth and development process of T. cinnabarinus with less impact on the environment and predatory mite (Neoseiulus barkeri), which can be used as a new target of RNAi for T. cinnabarinus.
期刊介绍：
Its scope covers all aspects of basic and applied research dealing with insects and more broadly with arthropods inhabiting wild, agricultural and/or urban habitats. The journal also considers research integrating various disciplines and issues within the broad field of entomology and ecology.
Entomologia Generalis publishes high quality research articles on advances in knowledge on the ecology and biology of arthropods, as well as on their importance for key ecosystems services, e.g. as biological control and pollination. The journal devotes special attention to contributions providing significant advances (i) on the fundamental knowledge and on sustainable control strategies of arthropod pests (including of stored products) and vectors of diseases, (ii) on the biology and ecology of beneficial arthropods, (iii) on the spread and impact of invasive pests, and (iv) on potential side effects of pest management methods.
Entomologia Generalis welcomes review articles on significant developments in the field of entomology. These are usually invited by the editorial board, but proposals may be sent to the Editor-in-Chief for preliminary assessment by the editorial board before formal submission to the journal. The journal also considers comments on papers published in Entomologia Generalis, as well as short notes on topics that are of broader interest.
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