An in-vitro cell culture system for accurately reproducing the coupled hemodynamic signals at the artery endothelium

IF 5.3 2区 医学 Q1 ENGINEERING, BIOMEDICAL Biocybernetics and Biomedical Engineering Pub Date : 2024-08-22 DOI:10.1016/j.bbe.2024.08.001
Lixue Liang, Xueying Wang, Dong Chen, Yanxia Wang, Xiaoyue Luo, Bo Liu, Yu Wang, Kai-rong Qin
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Abstract

Microfluidics has been an effective technology to reconstruct the in-vivo physiological hemodynamic microenvironment, which is significantly important for preventing and curing circulatory system-related diseases. However, these existing microfluidic systems have failed to accurately reproduce the arterial blood pressure, shear stress, circumferential strain, as well as their coupling relationship, and have not taken into account whether the cells at various locations in the culture chamber are subjected to consistent mechanical stimulation. To solve the above shortcomings, this study developed an in-vitro endothelial cell culture system (ECCS) containing a microfluidic chip and afterload components based on the hemodynamic principles to reappear the desired hemodynamic signals and their coupling relationship accurately, while a relatively uniform area of stress and strain distribution was selected in the microfluidic chip for a more reliable cell mechanobiology study. The sensitivity of global hemodynamic behaviors of the ECCS was analyzed, and numerical simulation and in-vitro experiments were implemented to verify the performance of the proposed ECCS. Finally, the cellular hemodynamic response was tested using human umbilical vein endothelial cells, demonstrating that the proposed in-vitro ECCS has better biological effectiveness. In general, the proposed ECCS in this study provided a more accurate and reliable tool for reproducing the in-vivo hemodynamic microenvironment and showed good potential in the mechanobiology study.
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准确再现动脉内皮耦合血液动力学信号的体外细胞培养系统
微流控技术是重建体内生理血流动力学微环境的有效技术,对预防和治疗循环系统相关疾病具有重要意义。然而,现有的这些微流控系统无法准确再现动脉血压、剪切应力、圆周应变及其耦合关系,也没有考虑到培养腔内不同位置的细胞是否受到一致的机械刺激。为了解决上述不足,本研究根据血流动力学原理开发了一种包含微流控芯片和后负荷组件的体外内皮细胞培养系统(ECCS),以准确再现所需的血流动力学信号及其耦合关系,同时在微流控芯片中选择了一个应力和应变分布相对均匀的区域,以进行更可靠的细胞机械生物学研究。分析了 ECCS 全局血液动力学行为的敏感性,并通过数值模拟和体外实验验证了所提出的 ECCS 的性能。最后,利用人体脐静脉内皮细胞对细胞血液动力学响应进行了测试,结果表明所提出的体外 ECCS 具有更好的生物有效性。总之,本研究中提出的 ECCS 为再现体内血液动力学微环境提供了更准确、更可靠的工具,在机械生物学研究中显示出良好的潜力。
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来源期刊
CiteScore
16.50
自引率
6.20%
发文量
77
审稿时长
38 days
期刊介绍: Biocybernetics and Biomedical Engineering is a quarterly journal, founded in 1981, devoted to publishing the results of original, innovative and creative research investigations in the field of Biocybernetics and biomedical engineering, which bridges mathematical, physical, chemical and engineering methods and technology to analyse physiological processes in living organisms as well as to develop methods, devices and systems used in biology and medicine, mainly in medical diagnosis, monitoring systems and therapy. The Journal''s mission is to advance scientific discovery into new or improved standards of care, and promotion a wide-ranging exchange between science and its application to humans.
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