Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioprocess Engineering Pub Date : 2024-08-21 DOI:10.1007/s12257-024-00132-3
Kilsun Myoung, Eun-Jeong Choi, Sehyun Kim, Jeong Ah Hwang, Ji Young Lee, Hyoung-June Kim, Jae Sung Hwang
{"title":"Nano-sized lysate of Lactiplantibacillus plantarum isolated from green tea leaves as a potential skin care ingredient","authors":"Kilsun Myoung, Eun-Jeong Choi, Sehyun Kim, Jeong Ah Hwang, Ji Young Lee, Hyoung-June Kim, Jae Sung Hwang","doi":"10.1007/s12257-024-00132-3","DOIUrl":null,"url":null,"abstract":"<p>Fermentation products and lysates of lactic acid bacteria (LAB) have been developed as cosmetic ingredients. The topical application of certain LAB strains can improve skin health and combat skin diseases. Here, we investigated the effects of nano-sized lysate of <i>Lactiplantibacillus plantarum</i> APsulloc 331261 (NLAP) isolated from green tea leaves on human skin cells and a reconstructed human epidermis (RHE) model. NLAP increased the expression of genes involved in skin barrier functions such as proliferation, differentiation, tight junction formation, and antimicrobial peptides in normal human epidermal keratinocytes. NLAP prevented the decrease in the expression of differentiation markers and increased release of inflammatory cytokines in keratinocytes cultured with <i>Staphylococcus aureus</i>. NLAP-induced improvements in gene expression and cytokine levels were also observed in RHE treated with heat-killed <i>S. aureus</i>. Additionally, the skin barrier-strengthening effect of NLAP was confirmed by comparing the penetration of the fluorescent dye into the RHE. These findings suggest that NLAP could aid skin barrier function, protect the skin against detrimental bacteria, and suppress inflammatory responses; thus, it can be developed as a skincare ingredient.</p>","PeriodicalId":8936,"journal":{"name":"Biotechnology and Bioprocess Engineering","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology and Bioprocess Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s12257-024-00132-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Fermentation products and lysates of lactic acid bacteria (LAB) have been developed as cosmetic ingredients. The topical application of certain LAB strains can improve skin health and combat skin diseases. Here, we investigated the effects of nano-sized lysate of Lactiplantibacillus plantarum APsulloc 331261 (NLAP) isolated from green tea leaves on human skin cells and a reconstructed human epidermis (RHE) model. NLAP increased the expression of genes involved in skin barrier functions such as proliferation, differentiation, tight junction formation, and antimicrobial peptides in normal human epidermal keratinocytes. NLAP prevented the decrease in the expression of differentiation markers and increased release of inflammatory cytokines in keratinocytes cultured with Staphylococcus aureus. NLAP-induced improvements in gene expression and cytokine levels were also observed in RHE treated with heat-killed S. aureus. Additionally, the skin barrier-strengthening effect of NLAP was confirmed by comparing the penetration of the fluorescent dye into the RHE. These findings suggest that NLAP could aid skin barrier function, protect the skin against detrimental bacteria, and suppress inflammatory responses; thus, it can be developed as a skincare ingredient.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
从绿茶叶中分离出的植物乳杆菌纳米级裂解物作为一种潜在的护肤成分
乳酸菌(LAB)的发酵产品和裂解物已被开发为化妆品成分。局部应用某些 LAB 菌株可以改善皮肤健康和防治皮肤疾病。在这里,我们研究了从绿茶叶中分离出来的植物乳杆菌 APsulloc 331261(NLAP)的纳米级裂解物对人类皮肤细胞和重建人类表皮(RHE)模型的影响。NLAP 增加了正常人表皮角质细胞中参与皮肤屏障功能的基因的表达,如增殖、分化、紧密连接形成和抗菌肽。NLAP 可防止用金黄色葡萄球菌培养的角质形成细胞中分化标志物表达的减少和炎症细胞因子释放的增加。在用热杀死的金黄色葡萄球菌处理的 RHE 中,也观察到了 NLAP 诱导的基因表达和细胞因子水平的改善。此外,通过比较荧光染料在 RHE 中的渗透情况,也证实了 NLAP 的皮肤屏障强化作用。这些研究结果表明,NLAP 可增强皮肤屏障功能,保护皮肤免受有害细菌的侵害,并抑制炎症反应,因此可将其开发为一种护肤成分。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biotechnology and Bioprocess Engineering
Biotechnology and Bioprocess Engineering 工程技术-生物工程与应用微生物
CiteScore
5.00
自引率
12.50%
发文量
79
审稿时长
3 months
期刊介绍: Biotechnology and Bioprocess Engineering is an international bimonthly journal published by the Korean Society for Biotechnology and Bioengineering. BBE is devoted to the advancement in science and technology in the wide area of biotechnology, bioengineering, and (bio)medical engineering. This includes but is not limited to applied molecular and cell biology, engineered biocatalysis and biotransformation, metabolic engineering and systems biology, bioseparation and bioprocess engineering, cell culture technology, environmental and food biotechnology, pharmaceutics and biopharmaceutics, biomaterials engineering, nanobiotechnology, and biosensor and bioelectronics.
期刊最新文献
Assessing the applicability of tunicate skin-extracted cellulose as a base material for ultrasound gel Fabrication of protein–inorganic biohybrid as an imageable drug delivery system comprising transferrin, green fluorescent protein, and copper phosphate Continuous cell recycling in methylotrophic yeast Pichia pastoris to enhance product yields: a case study with Yarrowia lipolytica lipase Lip2 Sensitive detection of SARS-CoV2 spike antibodies by a paper-based polypyrrole/reduced graphene oxide sensor A neural ordinary differential equation model for predicting the growth of Chinese Hamster Ovary cell in a bioreactor system
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1