Isolation of a halotolerant poly(ε-caprolactone)-depolymerizing strain of Bacillus gibsonii from seaside soil

Abstract

Few studies have investigated the biodegradation of microplastics in marine environments. Microorganisms that can degrade microplastics in high-salinity conditions are sought after. Therefore, we aimed to isolate a halotolerant poly(ε-caprolactone) (PCL)-degrading bacterium for applications in biotechnology. The bacterium isolated from seaside soil was identified as Bacillus gibsonii via phylogenetic analysis based on 16S rRNA gene sequences and designated as KRICT-1. We tested whether the KRICT-1 strain showed halotolerance by determining the sodium chloride (NaCl) tolerance at various concentrations. The KRICT-1 strain showed growth at up to 10% NaCl on Luria–Bertani (LB) medium agar plates and 10% NaCl in liquid LB medium, indicating that KRICT-1 can grow and produce a PCL-depolymerizing enzyme under high-salt conditions. The KRICT-1 strain could depolymerize PCL with a PCL film weight loss of 2.82% at up to 10% NaCl concentration after cultivation of 7 weeks. KRICT-1 is the first strain of B. gibsonii which shows PCL-depolymerizing activity. Scanning electron microscopy and water contact angle results confirmed the degradation of PCL by the KRICT-1 strain. The extracellular enzyme produced by the KRICT-1 strain was stable over a wide range of temperatures (15–40 °C) and pH (7.0–9.5). This halotolerant PCL-degrading bacterium can be used in the degradation of biodegradable plastics present in saline soils, saline water, and wastewater.

Graphical abstract