Investigating the Efficacy of Chitosan-Enriched Cuminum cyminum Essential Oil Against Food-Borne Molds, Aflatoxin B1, and Post-Harvest Quality of Arachis hypogaea L.
{"title":"Investigating the Efficacy of Chitosan-Enriched Cuminum cyminum Essential Oil Against Food-Borne Molds, Aflatoxin B1, and Post-Harvest Quality of Arachis hypogaea L.","authors":"Akshay Kumar, Tanya Singh Raghuvanshi, Vishal Gupta, Vivekanand, Niraj Kohar, Bhanu Prakash","doi":"10.1007/s11483-024-09877-z","DOIUrl":null,"url":null,"abstract":"<div><p>Nanoencapsulation of essential oils exhibited promising applications in food industries, especially in controlling spoilage due to food-borne microbes. In this study, the enhanced antimicrobial efficacy of nanoencapsulated <i>Cuminum cyminum</i> essential oil (Ne-CEO) against food-borne molds, and aflatoxin B<sub>1</sub> contamination was observed in a dose-dependent manner. The GC-MS results represent 14 different volatile organic compounds of (CEO) (94.49%), where cuminaldehyde was found to be the major one. The interaction of the <i>Cuminum cyminum</i> essential oil (CEO) and chitosan nanoparticles (CSNPs) was confirmed with the Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The Ne-CEO exhibited superior antimicrobial effects compared to non-encapsulated CEO and inhibited the growth of selected mold species (0.3–0.5 µL/mL) and aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) secretion at 0.4 µL/mL. The probable toxicity mechanism results show membrane impairment and cellular homeostasis linked with decreased ergosterol content, increased cation leakage, impairment in antioxidant defenses, carbon metabolism, and transcriptional genes (Ver-1 and Nor-1) functioning of AFB<sub>1</sub> biosynthesis. Furthermore, during the six months in-situ trial, Ne-CEO (0.4 µL/mL) remarkably protected the biodeterioration of <i>A. hypogaea</i> seed samples against <i>A. flavus</i> growth and AFB<sub>1</sub> contamination, thus enhancing its practical application as a plant-based food preservative to enhance the shelf-life of food commodities.</p></div>","PeriodicalId":564,"journal":{"name":"Food Biophysics","volume":"19 4","pages":"982 - 993"},"PeriodicalIF":2.8000,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Biophysics","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s11483-024-09877-z","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
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Abstract
Nanoencapsulation of essential oils exhibited promising applications in food industries, especially in controlling spoilage due to food-borne microbes. In this study, the enhanced antimicrobial efficacy of nanoencapsulated Cuminum cyminum essential oil (Ne-CEO) against food-borne molds, and aflatoxin B1 contamination was observed in a dose-dependent manner. The GC-MS results represent 14 different volatile organic compounds of (CEO) (94.49%), where cuminaldehyde was found to be the major one. The interaction of the Cuminum cyminum essential oil (CEO) and chitosan nanoparticles (CSNPs) was confirmed with the Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis. The Ne-CEO exhibited superior antimicrobial effects compared to non-encapsulated CEO and inhibited the growth of selected mold species (0.3–0.5 µL/mL) and aflatoxin B1 (AFB1) secretion at 0.4 µL/mL. The probable toxicity mechanism results show membrane impairment and cellular homeostasis linked with decreased ergosterol content, increased cation leakage, impairment in antioxidant defenses, carbon metabolism, and transcriptional genes (Ver-1 and Nor-1) functioning of AFB1 biosynthesis. Furthermore, during the six months in-situ trial, Ne-CEO (0.4 µL/mL) remarkably protected the biodeterioration of A. hypogaea seed samples against A. flavus growth and AFB1 contamination, thus enhancing its practical application as a plant-based food preservative to enhance the shelf-life of food commodities.
期刊介绍：
Biophysical studies of foods and agricultural products involve research at the interface of chemistry, biology, and engineering, as well as the new interdisciplinary areas of materials science and nanotechnology. Such studies include but are certainly not limited to research in the following areas: the structure of food molecules, biopolymers, and biomaterials on the molecular, microscopic, and mesoscopic scales; the molecular basis of structure generation and maintenance in specific foods, feeds, food processing operations, and agricultural products; the mechanisms of microbial growth, death and antimicrobial action; structure/function relationships in food and agricultural biopolymers; novel biophysical techniques (spectroscopic, microscopic, thermal, rheological, etc.) for structural and dynamical characterization of food and agricultural materials and products; the properties of amorphous biomaterials and their influence on chemical reaction rate, microbial growth, or sensory properties; and molecular mechanisms of taste and smell.
A hallmark of such research is a dependence on various methods of instrumental analysis that provide information on the molecular level, on various physical and chemical theories used to understand the interrelations among biological molecules, and an attempt to relate macroscopic chemical and physical properties and biological functions to the molecular structure and microscopic organization of the biological material.
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