Effects of Phenanthrene on Electrical Activity of Ventricular Cardiomyocytes in Atlantic Cod (Gadus morhua)

Pub Date : 2024-08-29 DOI:10.1134/s0022093024040033

T. S. Filatova, A. V. Shamshura, D. V. Abramochkin

{"title":"Effects of Phenanthrene on Electrical Activity of Ventricular Cardiomyocytes in Atlantic Cod (Gadus morhua)","authors":"T. S. Filatova, A. V. Shamshura, D. V. Abramochkin","doi":"10.1134/s0022093024040033","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Oil production in the Arctic shelf and its shipping via the\nNorthern Sea Route increase risks of pollution of Arctic ecosystems\nwith oil and its products. Polyaromatic hydrocarbons are known to\nbe the most toxic oil components, with phenanthrene being the most\nabundant among them and causing the most robust effects. Phenanthrene\nis known for its high toxicity for developing fish hearts, with\nits cardiotoxic effects being species-specific. Meanwhile, the effects\nof phenanthrene on cardiac function in Arctic fish, including commercially\nimportant fish species, are still poorly understood. Here, we studied the\neffects of phenanthrene on electrical activity and ionic currents\nin isolated ventricular cardiomyocytes of the Atlantic cod (Gadus morhua) using the patch clamp\ntechnique. The major ionic currents in cod myocardium were IKr,\nIK1, INa and ICa.\nPhenanthrene at a concentration of 1 µM (1) had no effect on the\nduration of action potentials (APs), (2) suppressed rapid delayed\nrectifier K+ current IKr by\n61.33 ± 3.94%, thus decreasing the repolarization reserve in cardiomyocytes,\n(3) had no effect on either the resting potential level or background\ninward rectifier K+ current (IK1),\n(4) decreased AP upstroke velocity due to suppression of fast Na+ current\n(INa), (5) insignificantly reduced the amplitude\nof Ca2+ current (ICa)\nand accelerated its inactivation, which overall led to a decrease\nin ICa total cumulative charge transfer.\nThus, the effects of phenanthrene on cod myocardium at a cellular\nlevel can be characterized as potentially proarrhythmic, which makes\ncod populations in the Arctic seas vulnerable to pollution of the\naquatic environment with toxic oil components during oil spills\nin the event of man-made disasters.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024040033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Oil production in the Arctic shelf and its shipping via the Northern Sea Route increase risks of pollution of Arctic ecosystems with oil and its products. Polyaromatic hydrocarbons are known to be the most toxic oil components, with phenanthrene being the most abundant among them and causing the most robust effects. Phenanthrene is known for its high toxicity for developing fish hearts, with its cardiotoxic effects being species-specific. Meanwhile, the effects of phenanthrene on cardiac function in Arctic fish, including commercially important fish species, are still poorly understood. Here, we studied the effects of phenanthrene on electrical activity and ionic currents in isolated ventricular cardiomyocytes of the Atlantic cod (Gadus morhua) using the patch clamp technique. The major ionic currents in cod myocardium were I_Kr, I_K1, I_Na and I_Ca. Phenanthrene at a concentration of 1 µM (1) had no effect on the duration of action potentials (APs), (2) suppressed rapid delayed rectifier K⁺ current I_Kr by 61.33 ± 3.94%, thus decreasing the repolarization reserve in cardiomyocytes, (3) had no effect on either the resting potential level or background inward rectifier K⁺ current (I_K1), (4) decreased AP upstroke velocity due to suppression of fast Na⁺ current (I_Na), (5) insignificantly reduced the amplitude of Ca²⁺ current (I_Ca) and accelerated its inactivation, which overall led to a decrease in I_Ca total cumulative charge transfer. Thus, the effects of phenanthrene on cod myocardium at a cellular level can be characterized as potentially proarrhythmic, which makes cod populations in the Arctic seas vulnerable to pollution of the aquatic environment with toxic oil components during oil spills in the event of man-made disasters.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

菲对大西洋鳕鱼（Gadus morhua）心室心肌细胞电活动的影响

摘要北极大陆架的石油生产及其通过北海航线的运输增加了石油及其产品污染北极生态系统的风险。众所周知，多芳烃是毒性最强的石油成分，其中菲含量最高，造成的影响也最大。众所周知，菲对发育中的鱼类心脏有剧毒，其心脏毒性作用具有物种特异性。与此同时，人们对菲对北极鱼类（包括重要的商业鱼类）心脏功能的影响还知之甚少。在此，我们利用贴片钳技术研究了菲对大西洋鳕鱼（Gadus morhua）离体心室心肌细胞电活动和离子电流的影响。浓度为 1 µM 的菲 (1) 对动作电位（AP）的持续时间没有影响，(2) 抑制了快速延迟整流 K+ 电流 IKr，抑制率为 61.33 ± 3.94%，从而降低了心肌细胞的复极化储备；(3) 对静息电位水平和背景内向整流 K+ 电流（IK1）均无影响；(4) 由于抑制了快速 Na+ 电流（INa），降低了 AP 上冲速度；(5) 显著降低了 Ca2+ 电流（ICa）的幅值并加速了其失活，总体上导致了 ICa 总累积电荷转移的减少。因此，菲在细胞水平上对鳕鱼心肌的影响可归结为潜在的促心律失常，这使得北极海域的鳕鱼种群在发生人为灾难时，容易受到石油泄漏时有毒石油成分对水生环境的污染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助