Designing magnetic microcapsules for cultivation and differentiation of stem cell spheroids

IF 7.3 1区工程技术 Q1 INSTRUMENTS & INSTRUMENTATION Microsystems & Nanoengineering Pub Date : 2024-09-12 DOI:10.1038/s41378-024-00747-9

Kihak Gwon, Ether Dharmesh, Kianna M. Nguyen, Anna Marie R. Schornack, Jose M. de Hoyos-Vega, Hakan Ceylan, Gulnaz Stybayeva, Quinn P. Peterson, Alexander Revzin

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Abstract

Human pluripotent stem cells (hPSCs) represent an excellent cell source for regenerative medicine and tissue engineering applications. However, there remains a need for robust and scalable differentiation of stem cells into functional adult tissues. In this paper, we sought to address this challenge by developing magnetic microcapsules carrying hPSC spheroids. A co-axial flow-focusing microfluidic device was employed to encapsulate stem cells in core-shell microcapsules that also contained iron oxide magnetic nanoparticles (MNPs). These microcapsules exhibited excellent response to an external magnetic field and could be held at a specific location. As a demonstration of utility, magnetic microcapsules were used for differentiating hPSC spheroids as suspension cultures in a stirred bioreactor. Compared to standard suspension cultures, magnetic microcapsules allowed for more efficient media change and produced improved differentiation outcomes. In the future, magnetic microcapsules may enable better and more scalable differentiation of hPSCs into adult cell types and may offer benefits for cell transplantation.

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设计用于干细胞球体培养和分化的磁性微胶囊

人类多能干细胞（hPSCs）是再生医学和组织工程应用的绝佳细胞来源。然而，仍然需要将干细胞稳健、可扩展地分化为功能性成体组织。在本文中，我们试图通过开发携带 hPSC 球体的磁性微囊来应对这一挑战。我们采用了一种同轴流聚焦微流控装置，将干细胞封装在同时含有氧化铁磁性纳米颗粒（MNPs）的核壳微囊中。这些微胶囊对外部磁场有极好的反应，并能被固定在特定位置。磁性微胶囊在搅拌式生物反应器中作为悬浮培养物用于分化 hPSC 球形细胞。与标准悬浮培养物相比，磁性微囊能更有效地更换培养基，并产生更好的分化结果。未来，磁性微胶囊可能会使 hPSCs 更好、更可扩展地分化成成体细胞类型，并为细胞移植带来益处。

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来源期刊

Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)

CiteScore

12.00

自引率

3.80%

发文量

123

审稿时长

20 weeks

期刊介绍： Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.