{"title":"Polymorphic microsatellite markers for genetic analysis of Berkeleyomyces rouxiae, a causal agent of black root rot","authors":"Akari Oya, Toshiyuki Usami","doi":"10.1007/s10327-024-01198-w","DOIUrl":null,"url":null,"abstract":"<p><i>Berkeleyomyces rouxiae</i>, a soil-borne fungal pathogen, causes black root rot on various plant species including carrot, okra, tobacco, lettuce, and other important crops. Here we analyzed the entire genome sequence of a Japanese isolate of <i>B. rouxiae</i> and discovered 25 microsatellite sequences. These sequences were amplified from the genomic DNA of several Japanese isolates by PCR and sequenced, revealing that the microsatellite sequences were polymorphic among isolates. These markers are useful for phylogenetic analysis, epidemiological population analysis, identification of specific isolates, and other genetic investigation of <i>B. rouxiae</i>.</p>","PeriodicalId":15825,"journal":{"name":"Journal of General Plant Pathology","volume":"80 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of General Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10327-024-01198-w","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Berkeleyomyces rouxiae, a soil-borne fungal pathogen, causes black root rot on various plant species including carrot, okra, tobacco, lettuce, and other important crops. Here we analyzed the entire genome sequence of a Japanese isolate of B. rouxiae and discovered 25 microsatellite sequences. These sequences were amplified from the genomic DNA of several Japanese isolates by PCR and sequenced, revealing that the microsatellite sequences were polymorphic among isolates. These markers are useful for phylogenetic analysis, epidemiological population analysis, identification of specific isolates, and other genetic investigation of B. rouxiae.
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The Journal of General Plant Pathology welcomes all manuscripts dealing with plant diseases or their control, including pathogen characterization, identification of pathogens, disease physiology and biochemistry, molecular biology, morphology and ultrastructure, genetics, disease transmission, ecology and epidemiology, chemical and biological control, disease assessment, and other topics relevant to plant pathological disorders.
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