Green synthesis and characterization studies of TiO2 nanoparticles and its potential biological performance

Abstract

Recent interest was been sparked by the potential of eco-friendly nanomaterials against human pathogenic bacteria’s and cancer cells. In the first method, TiO was obtained from green synthesis method by using seed extraction. In the second method, TiO nanoparticles were chemically synthesized by using the sol-gel process. Powder XRD, UV-Vis, ATR-IR, FESEM with EDAX, and HR-TEM were used to analyze the characteristics of the synthesized TiO nanoparticles. XRD results showed the well-developed crystallized smaller particles. The sizes of the crystalline particles were estimated by using Size-Strain Plot (SSP). The disc diffusion method was used to examine the antibacterial activity of TiO nanoparticles against gram bacteria. The synthesized TiO nanoparticles showed the largest inhibition zone and found to be more antibacterial against gram-negative bacteria. When using the green synthesized nanoparticles at a dose of 1000 µg/mL, the largest zone of inhibition against measured 15 mm. Then, the chemically synthesized TiO had the same concentration exhibited 7 mm inhibition zone. Cytotoxicity activities of human breast cancer cell were evolved by using MTT assay and their observation were showed more efficient cytotoxicity of green synthesized TiO nanoparticles compared to the chemically synthesized TiO nanoparticles. The antioxidant activity potential of green and chemically synthesized titanium dioxide nanoparticles was examined and the maximum inhibition in 400 µg/mL concentrations was reported to be 53 % and 45 %, respectively. Bovine Serum Albumin (BSA) denaturation technique revealed the significant anti-inflammatory action of green synthesized TiO, with protein denaturation of egg albumin determined as 92.2 % at 400 µg/mL concentration.