Prevalence and molecular characterization of multidrug-resistant coagulase negative staphylococci from urban wastewater in Delhi-NCR, India

IF 2.6 3区生物学 Q3 MICROBIOLOGY Archives of Microbiology Pub Date : 2024-09-10 DOI:10.1007/s00203-024-04124-y

Sonali Rajput, Sayani Mitra, Aftab Hossain Mondal, Himani Kumari, Kasturi Mukhopadhyay

{"title":"Prevalence and molecular characterization of multidrug-resistant coagulase negative staphylococci from urban wastewater in Delhi-NCR, India","authors":"Sonali Rajput, Sayani Mitra, Aftab Hossain Mondal, Himani Kumari, Kasturi Mukhopadhyay","doi":"10.1007/s00203-024-04124-y","DOIUrl":null,"url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is global health concern escalating rapidly in both clinical settings and environment. The effluent from pharmaceuticals and hospitals may contain diverse antibiotics, exerting selective pressure to develop AMR. To study the aquatic prevalence of drug-resistant staphylococci, sampling was done from river Yamuna (3 sites) and wastewater (7 sites) near pharmaceutical industries in Delhi-NCR, India. 59.25% (224/378) were considered presumptive staphylococci while, methicillin resistance was noted in 25% (56/224) isolates. Further, 23 methicillin-resistant coagulase negative staphylococci (MR-CoNS) of 8 different species were identified via 16S rRNA gene sequencing. Multidrug resistance (MDR) was noted in 60.87% (14/23) isolates. PCR based detection of antibiotic resistance genes revealed the number of isolates containing <i>mecA</i> (7/23), <i>blaZ</i> (6/23), <i>msrA</i> (10/23), <i>aac</i><i>(6′)</i><i>aph</i><i> (2”)</i> (2/23), <i>aph</i><i>(3′)</i>-<i>IIIa</i> (2/23), <i>ant(4′)-Ia</i> (1/23), <i>dfrG</i> (4/23), <i>dfrA</i>(<i>drf</i><i>S1</i>) (3/23), <i>tetK</i> (1/23) and <i>tetM</i> (1/23). The current research highlights the concerning prevalence of MDR-CoNS in aquatic environment in Delhi.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 10","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04124-y.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04124-y","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Antimicrobial resistance (AMR) is global health concern escalating rapidly in both clinical settings and environment. The effluent from pharmaceuticals and hospitals may contain diverse antibiotics, exerting selective pressure to develop AMR. To study the aquatic prevalence of drug-resistant staphylococci, sampling was done from river Yamuna (3 sites) and wastewater (7 sites) near pharmaceutical industries in Delhi-NCR, India. 59.25% (224/378) were considered presumptive staphylococci while, methicillin resistance was noted in 25% (56/224) isolates. Further, 23 methicillin-resistant coagulase negative staphylococci (MR-CoNS) of 8 different species were identified via 16S rRNA gene sequencing. Multidrug resistance (MDR) was noted in 60.87% (14/23) isolates. PCR based detection of antibiotic resistance genes revealed the number of isolates containing mecA (7/23), blaZ (6/23), msrA (10/23), aac(6′)aph (2”) (2/23), aph(3′)-IIIa (2/23), ant(4′)-Ia (1/23), dfrG (4/23), dfrA(drfS1) (3/23), tetK (1/23) and tetM (1/23). The current research highlights the concerning prevalence of MDR-CoNS in aquatic environment in Delhi.

Graphical abstract

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

印度德里-西北地区城市污水中耐多药凝固酶阴性葡萄球菌的流行情况和分子特征

抗菌素耐药性（AMR）是一个全球健康问题，在临床和环境中都在迅速升级。制药厂和医院排出的污水中可能含有多种抗生素，这对产生 AMR 造成了选择性压力。为了研究耐药葡萄球菌在水生环境中的流行情况，我们在印度德里-北卡罗来纳州制药工业附近的亚穆纳河（3 个地点）和废水（7 个地点）中进行了采样。59.25%（224/378）的分离物被认为是推定葡萄球菌，而 25%（56/224）的分离物对甲氧西林产生了耐药性。此外，通过 16S rRNA 基因测序，确定了 8 个不同种类的 23 株耐甲氧西林凝固酶阴性葡萄球菌（MR-CoNS）。60.87%（14/23）的分离物具有耐多药（MDR）性。基于 PCR 的抗生素耐药基因检测显示，分离物中含有 mecA (7/23)、blaZ (6/23)、msrA (10/23)、aac(6′)aph (2") (2/23)、aph(3′)-IIIa (2/23)、ant(4′)-Ia (1/23)、dfrG (4/23)、dfrA(drfS1) (3/23)、tetK (1/23) 和 tetM (1/23)。目前的研究突显了德里水生环境中 MDR-CoNS 的流行情况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.