{"title":"Transcriptomic mapping of the 5-HT receptor landscape","authors":"Roberto De Filippo, Dietmar Schmitz","doi":"10.1016/j.patter.2024.101048","DOIUrl":null,"url":null,"abstract":"<p>Serotonin (5-HT) is crucial for regulating brain functions such as mood, sleep, and cognition. This study presents a comprehensive transcriptomic analysis of 5-HT receptors (Htrs) across ≈4 million cells in the adult mouse brain using single-cell RNA sequencing (scRNA-seq) data from the Allen Institute. We observed differential transcription patterns of all 14 Htr subtypes, revealing diverse prevalence and distribution across cell classes. Remarkably, we found that 65.84% of cells transcribe RNA of at least one Htr, with frequent co-transcription of multiple Htrs, underscoring the complexity of the 5-HT system even at the single-cell dimension. Leveraging a multiplexed error-robust fluorescence <em>in situ</em> hybridization (MERFISH) dataset provided by Harvard University of ≈10 million cells, we analyzed the spatial distribution of each Htr, confirming previous findings and uncovering novel transcription patterns. To aid in exploring Htr transcription, we provide an online interactive visualizer.</p>","PeriodicalId":36242,"journal":{"name":"Patterns","volume":null,"pages":null},"PeriodicalIF":6.7000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Patterns","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.patter.2024.101048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"COMPUTER SCIENCE, ARTIFICIAL INTELLIGENCE","Score":null,"Total":0}
引用次数: 0
Abstract
Serotonin (5-HT) is crucial for regulating brain functions such as mood, sleep, and cognition. This study presents a comprehensive transcriptomic analysis of 5-HT receptors (Htrs) across ≈4 million cells in the adult mouse brain using single-cell RNA sequencing (scRNA-seq) data from the Allen Institute. We observed differential transcription patterns of all 14 Htr subtypes, revealing diverse prevalence and distribution across cell classes. Remarkably, we found that 65.84% of cells transcribe RNA of at least one Htr, with frequent co-transcription of multiple Htrs, underscoring the complexity of the 5-HT system even at the single-cell dimension. Leveraging a multiplexed error-robust fluorescence in situ hybridization (MERFISH) dataset provided by Harvard University of ≈10 million cells, we analyzed the spatial distribution of each Htr, confirming previous findings and uncovering novel transcription patterns. To aid in exploring Htr transcription, we provide an online interactive visualizer.
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