Bio-functionalized conductive poly(acrylic acid):poly(3,4-Ethylenedioxythiophene)-Prussian blue hybrid transducer for biosensors and bioelectronics interfaces

IF 6.7 2区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Materials Today Chemistry Pub Date : 2024-08-29 DOI:10.1016/j.mtchem.2024.102271
Kiattisak Promsuwan, Jenjira Saichanapan, Asamee Soleh, Kasrin Saisahas, Kritsada Samoson, Sangay Wangchuk, Warakorn Limbut
{"title":"Bio-functionalized conductive poly(acrylic acid):poly(3,4-Ethylenedioxythiophene)-Prussian blue hybrid transducer for biosensors and bioelectronics interfaces","authors":"Kiattisak Promsuwan, Jenjira Saichanapan, Asamee Soleh, Kasrin Saisahas, Kritsada Samoson, Sangay Wangchuk, Warakorn Limbut","doi":"10.1016/j.mtchem.2024.102271","DOIUrl":null,"url":null,"abstract":"This study presents an innovative organic-inorganic hybrid transducer of poly(acrylic acid) (PAA), poly(3,4-ethylenedioxythiophene) (PEDOT) and Prussian blue (PB) nanocatalysts. The transducer demonstrated functionality conducive to enzyme conjugation and exhibited favorable electrochemical properties for biosensor signal transduction. Fabricated by a one-tube chemical redox method, the PAA:PEDOT-PB transducer showed long-term electrocatalytic and structural stability. The performance of the transducer was characterized by high transduction activity and low charge transfer resistance, particularly for HO reduction. It achieves a linear detection range from 1.0 μM to 4.0 mM, with a sensitivity of 494 ± 10 μA mM cm and a LOD of 0.34 μM. The PAA:PEDOT-PB transducer featured a high density of carboxyl groups (D = 14.64 ± 0.05 μmol cm) that promoted the immobilization of the HO-dependent oxidase enzyme lactate oxidase (LOx) with an EDC/S–NHS coupling agent. The LOx-PAA:PEDOT-PB transducer was developed for lactate biosensing. The transducer provided high LOx-enzyme affinity (K = 1.47 ± 0.05 mM), and a rapid response time (10 s) for lactate detection across a concentration range of 5.0 μM to 4.0 mM, showing a sensitivity of 223 ± 3 μA mM cm and an LOD of 1.45 μM. The LOx-PAA:PEDOT-PB transducer was integrated with a flexible screen-printed electrode, incorporating a wireless, battery-free near-field communication potentiostat module to measure lactate in artificial sweat on a skin model via smartphone. The PAA:PEDOT-PB transducer could enable connections with multiple bio-recognition molecules through polycarboxylic acid groups, providing potential avenues for the development of advanced biosensors.","PeriodicalId":18353,"journal":{"name":"Materials Today Chemistry","volume":"5 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materials Today Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.mtchem.2024.102271","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

This study presents an innovative organic-inorganic hybrid transducer of poly(acrylic acid) (PAA), poly(3,4-ethylenedioxythiophene) (PEDOT) and Prussian blue (PB) nanocatalysts. The transducer demonstrated functionality conducive to enzyme conjugation and exhibited favorable electrochemical properties for biosensor signal transduction. Fabricated by a one-tube chemical redox method, the PAA:PEDOT-PB transducer showed long-term electrocatalytic and structural stability. The performance of the transducer was characterized by high transduction activity and low charge transfer resistance, particularly for HO reduction. It achieves a linear detection range from 1.0 μM to 4.0 mM, with a sensitivity of 494 ± 10 μA mM cm and a LOD of 0.34 μM. The PAA:PEDOT-PB transducer featured a high density of carboxyl groups (D = 14.64 ± 0.05 μmol cm) that promoted the immobilization of the HO-dependent oxidase enzyme lactate oxidase (LOx) with an EDC/S–NHS coupling agent. The LOx-PAA:PEDOT-PB transducer was developed for lactate biosensing. The transducer provided high LOx-enzyme affinity (K = 1.47 ± 0.05 mM), and a rapid response time (10 s) for lactate detection across a concentration range of 5.0 μM to 4.0 mM, showing a sensitivity of 223 ± 3 μA mM cm and an LOD of 1.45 μM. The LOx-PAA:PEDOT-PB transducer was integrated with a flexible screen-printed electrode, incorporating a wireless, battery-free near-field communication potentiostat module to measure lactate in artificial sweat on a skin model via smartphone. The PAA:PEDOT-PB transducer could enable connections with multiple bio-recognition molecules through polycarboxylic acid groups, providing potential avenues for the development of advanced biosensors.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
生物功能化导电聚(丙烯酸):聚(3,4-亚乙二氧基噻吩)-普鲁士蓝混合传感器,用于生物传感器和生物电子学接口
本研究提出了一种创新的有机-无机混合传感器,由聚(丙烯酸)(PAA)、聚(3,4-亚乙二氧基噻吩)(PEDOT)和普鲁士蓝(PB)纳米催化剂组成。这种传感器具有有利于酶连接的功能,并表现出有利于生物传感器信号传导的电化学特性。通过单管化学氧化还原法制作的 PAA:PEDOT-PB 传感器显示出长期的电催化和结构稳定性。该传感器的性能特点是高转导活性和低电荷转移电阻,尤其是在 HO 还原方面。它的线性检测范围为 1.0 μM 至 4.0 mM,灵敏度为 494 ± 10 μA mM cm,LOD 为 0.34 μM。PAA:PEDOT-PB 传感器具有高密度的羧基(D = 14.64 ± 0.05 μmol cm),可促进依赖于 HO 的氧化酶乳酸氧化酶(LOx)与 EDC/S-NHS 偶联剂的固定。开发出了用于乳酸盐生物传感的 LOx-PAA:PEDOT-PB 传感器。该传感器具有较高的 LOx 酶亲和性(K = 1.47 ± 0.05 mM)和快速响应时间(10 秒),可在 5.0 μM 至 4.0 mM 的浓度范围内检测乳酸盐,灵敏度为 223 ± 3 μA mM cm,LOD 为 1.45 μM。LOx-PAA:PEDOT-PB传感器与柔性丝网印刷电极相集成,结合了无线、免电池近场通信恒电位仪模块,可通过智能手机在皮肤模型上测量人工汗液中的乳酸盐。PAA:PEDOT-PB 传感器可通过聚羧酸基团与多种生物识别分子连接,为开发先进的生物传感器提供了潜在的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
8.90
自引率
6.80%
发文量
596
审稿时长
33 days
期刊介绍: Materials Today Chemistry is a multi-disciplinary journal dedicated to all facets of materials chemistry. This field represents one of the fastest-growing areas of science, involving the application of chemistry-based techniques to the study of materials. It encompasses materials synthesis and behavior, as well as the intricate relationships between material structure and properties at the atomic and molecular scale. Materials Today Chemistry serves as a high-impact platform for discussing research that propels the field forward through groundbreaking discoveries and innovative techniques.
期刊最新文献
Light-responsive biowaste-derived and bio-inspired textiles: Dancing between bio-friendliness and antibacterial functionality NiFe2O4 magnetic nanoparticles supported on MIL-101(Fe) as bimetallic adsorbent for boosted capture ability toward levofloxacin Recent advances in the preparation and application of graphene oxide smart response membranes The potential of collagen-based materials for wound management Development of Mg2TiO4:Mn4+ phosphors for enhanced red LED emission and forensic fingerprint analysis
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1