Gold Nanoparticles Supported Over Biodegradable gum acacia Modified Magnetic Nanoparticles: Characterization and Evaluation of Antioxidant, Cytotoxicity, and Anti-Rheumatoid Arthritic Properties

Abstract

In recent times, numerous studies have unveiled the ability of plants to enhance the cytotoxicity and therapeutic capabilities of gold nanoparticles. The development and formulation of novel therapeutic supplements or medications for disease treatment are currently at the forefront of research endeavors in both developing and developed nations. In this context an environmentally friendly process was token up to keep up Au NPs over gum acacia (GA) adapted magnetic nanoparticles (Fe₃O₄-GA/Au NPs) with anti-rheumatoid arthritis property. Within this gradual adjustment approach, the pre-synthesized Fe₃O₄ NPs were covered by the gum acacia (GA) having hydroxyl groups on its lynchpin applicable in cresting, decreasing and stabilizing factor for the kept up Au nanoparticles. The crested Au ions were lowered and kept over the acacia gum due to hydrogen binding of the hydroxyl groups. The concluding bio-material characteristics were drawn through numerous analytical methods including EDX, XRD, ICP-OES, TEM, FE-SEM and FT-IR. The anti-rheumatoid arthritic efficacy was assessed in live subjects using Complete Freund Adjuvant (CFA), formaldehyde, and turpentine oil models at doses of 80 and 160 µg/kg. The anti-rheumatoid arthritic effect of Fe₃O₄-GA/Au NPs was found to be dependent on the dosage in both turpentine oil and formaldehyde models. The highest level of activity was observed at a dosage of 160 µg/kg. The CFA model’s findings illustrated enhanced safeguarding against changes in body weight and arthritic lesions. Furthermore, Fe₃O₄-GA/Au NPs significantly improved rheumatoid factor, abnormal hematological parameters, and positively influenced histopathological and radiographic alterations. Based on the aforementioned results, it is possible to utilize the Fe₃O₄-GA/Au NPs in human subjects to address various forms of rheumatoid arthritis.