Paradise fish (Macropodus opercularis) as novel translational model for emotional and cognitive function

Abstract

Zebrafish have revolutionised physiological screening in vertebrates, but the interpretation of individual-based behavioural assays is burdened by the strong sociality of this species. We propose the use of a solitaire fish species, the paradise fish to keep the advantages and compensate for the limitations of the zebrafish model. We compared juvenile paradise fish and zebrafish in social and non-social exploratory tasks, anxiety tests and in a working memory assay to assess their performance in these individual-based models. We found that in contrast to zebrafish, paradise fish did not show social approach in the U-shape sociability test, their novelty exploration was not biased by the presence of a conspecific in the slalom test, and was not impaired by social isolation in the showjump or in the swimming plus-maze (SPM) anxiety tests. While social circumstances did not affect the anxiety of paradise fish, it was sensitive to the anxiolytic drug buspirone. Intra- and interest repeatability measures of the anxiety tests revealed that paradise fish express more consistent exploratory and defensive behaviours regarding time and context compared to zebrafish. Behavioural consistency in paradise fish was also supported by arm alternation as a predominant choice of exploration in the y-maze task. In summary, our results indicate that the behaviour of paradise fish is less biased by social cues and is more effective and repeatable in individual-based assays than zebrafish behaviour. We hypothesise that the two phenomena are connected and novelty exploration, anxiety and working memory can be more reliably measured and are translatable in a solitaire species.