Product catalysis dual entropy-driven amplification reaction strategy for miRNA-21 detection in glioblastoma

Abstract

MicroRNA is taken as diagnostic tumor markers in clinical diagnosis of various cancers. However, the complexity system and low utilization rate of entropy-driven amplification reaction (EDAR) limit its application. In this work, a product catalysis dual EDAR amplification strategy is developed for miRNA-21 detection. This strategy allows miRNA-21 to simultaneously catalyze two EDARs, greatly increasing the limit of detection to 0.40 pM and enhancing amplification efficiency. The amplification efficiency was also significantly enhanced with a much shorter reaction time by increasing concentration of the catalyzer (EDAR product). The approach was successfully applied in clinical samples, showing potential for early screening and diagnosis.