Pub Date : 2024-09-12DOI: 10.1016/j.microc.2024.111575
Tao Yang, Jinjia Li, Chenhui Qiao, Xi Xiao, Jitao Wan, Weihua Hong, Chao Zhang, Junyuan Tang, Feng Gao
The surface chemical state of the electrode modification material, particularly crystal defects, significantly affects electrochemical detection properties. In this study, TiC decorated by Pt nanoaggregates (Pt NAs-TiC) with enhanced crystal defect was developed for quercetin detection, where Pt nanoaggregates on TiC exhibit improved dispersibility and smaller particle size, generating a more favorable environment for interfacial catalytic reactions. A large specific surface area, the Pt NAs-TiC composite is beneficial for the adsorption and transfer of quercetin on the electrode surface. In comparison to Pt nanoaggregates with complete crystals, the Pt NA-TiC-modified glassy carbon electrode (Pt NA-TiC/GCE) demonstrates superior quercetin detection capabilities, achieving a detection sensitivity of 72.3 μA μM and an outstanding detection linear region of 0.1–1500 nM. Besides, the electrode showcases excellent reproducibility with RSD of 2.41 %, repeatability with RSD of 1.08 %, and long-term stability with 92.3 % of the original signal retained, and resistance to interference, achieving accurately and selectively quercetin detection even in the presence of other ions and compounds. In addition, the Pt NA-TiC/GCE allows for precise quercetin detection in real-life samples. Our findings establish a theoretical guideline and furnish the material foundation for the development of high-performance electrochemical sensors.
{"title":"Electrochemical detection of quercetin at a Pt nanoaggregate-decorated Ti3C2-modified electrode","authors":"Tao Yang, Jinjia Li, Chenhui Qiao, Xi Xiao, Jitao Wan, Weihua Hong, Chao Zhang, Junyuan Tang, Feng Gao","doi":"10.1016/j.microc.2024.111575","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111575","url":null,"abstract":"The surface chemical state of the electrode modification material, particularly crystal defects, significantly affects electrochemical detection properties. In this study, TiC decorated by Pt nanoaggregates (Pt NAs-TiC) with enhanced crystal defect was developed for quercetin detection, where Pt nanoaggregates on TiC exhibit improved dispersibility and smaller particle size, generating a more favorable environment for interfacial catalytic reactions. A large specific surface area, the Pt NAs-TiC composite is beneficial for the adsorption and transfer of quercetin on the electrode surface. In comparison to Pt nanoaggregates with complete crystals, the Pt NA-TiC-modified glassy carbon electrode (Pt NA-TiC/GCE) demonstrates superior quercetin detection capabilities, achieving a detection sensitivity of 72.3 μA μM and an outstanding detection linear region of 0.1–1500 nM. Besides, the electrode showcases excellent reproducibility with RSD of 2.41 %, repeatability with RSD of 1.08 %, and long-term stability with 92.3 % of the original signal retained, and resistance to interference, achieving accurately and selectively quercetin detection even in the presence of other ions and compounds. In addition, the Pt NA-TiC/GCE allows for precise quercetin detection in real-life samples. Our findings establish a theoretical guideline and furnish the material foundation for the development of high-performance electrochemical sensors.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-11DOI: 10.1016/j.microc.2024.111587
Oskay Kahraman, Ersan Turunc, Riza Binzet
In this study, Pers., which is an important holoparasite plant, was used for green synthesis of reduced graphene oxide silver nanocomposites (rGO-AgNCs). The obtained rGO-AgNCs were characterizated using UV–Vis spectroscopy, Fourier transformation infrared spectroscopy (FT-IR), X-Ray diffractiometer (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), and X-Ray photoelectron spectroscopy (XPS). The synthesized rGO-AgNCs structure were used to modify carbon paste electrode (rGO-AgNCs@CPE) for electrochemical determination of metronidazole (MNZ). It was observed that the obtained rGO-AgNCs@CPE showed higher sensitivity in MNZ determination than the unmodified CPE, and DPV measurements showed a low detection limit of 0.039 μM over a linear concentration range of 5–300 μM. Real sample analysis of the proposed sensor shows that it responds well in MNZ analysis of human urine.
{"title":"Eco-friendly Synthesis of Silver-Doped Reduced Graphene Oxide Nanocomposite, The Characterization, and Evaluation of Electrochemical Activity","authors":"Oskay Kahraman, Ersan Turunc, Riza Binzet","doi":"10.1016/j.microc.2024.111587","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111587","url":null,"abstract":"In this study, Pers., which is an important holoparasite plant, was used for green synthesis of reduced graphene oxide silver nanocomposites (rGO-AgNCs). The obtained rGO-AgNCs were characterizated using UV–Vis spectroscopy, Fourier transformation infrared spectroscopy (FT-IR), X-Ray diffractiometer (XRD), Scanning electron microscope (SEM), Transmission electron microscope (TEM), and X-Ray photoelectron spectroscopy (XPS). The synthesized rGO-AgNCs structure were used to modify carbon paste electrode (rGO-AgNCs@CPE) for electrochemical determination of metronidazole (MNZ). It was observed that the obtained rGO-AgNCs@CPE showed higher sensitivity in MNZ determination than the unmodified CPE, and DPV measurements showed a low detection limit of 0.039 μM over a linear concentration range of 5–300 μM. Real sample analysis of the proposed sensor shows that it responds well in MNZ analysis of human urine.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Overuse of benzoate as a preservative is bad for human health. Therefore, we need to achieve quantitative detection of benzoate. Colorimetric nanozyme sensors have garnered significant interest owing to their exceptional efficacy and affordability. In this article, Fe-N-C dodecahedral nanozymes with superior peroxidase-like catalytic activity are prepared using ZIF-8 as a precursor. Therefore, we construct a colorimetric sensor for benzoate detection with a detection limit of 0.33 μM through integrating the inhibitory impact of benzoate on D-amino acid oxidase activity with the Fe-N-C-catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)–HO system, and this reaction is completed within 10 min. It is noteworthy that a recovery rate of 96.8–109 % has been attained for the detection of benzoate in beverages.
{"title":"Colorimetric detection of benzoate in the beverage using the peroxidase-like properties of single-atom Fe-N-C nanozymes","authors":"Shuo Qin, Zongze Li, Boran Zhou, Bin Liu, Yuting Xue, Ruixue Zhao, Lirong Zheng, Zhengbo Chen, Xia Zuo","doi":"10.1016/j.microc.2024.111614","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111614","url":null,"abstract":"Overuse of benzoate as a preservative is bad for human health. Therefore, we need to achieve quantitative detection of benzoate. Colorimetric nanozyme sensors have garnered significant interest owing to their exceptional efficacy and affordability. In this article, Fe-N-C dodecahedral nanozymes with superior peroxidase-like catalytic activity are prepared using ZIF-8 as a precursor. Therefore, we construct a colorimetric sensor for benzoate detection with a detection limit of 0.33 μM through integrating the inhibitory impact of benzoate on D-amino acid oxidase activity with the Fe-N-C-catalyzed 3,3′,5,5′-tetramethylbenzidine (TMB)–HO system, and this reaction is completed within 10 min. It is noteworthy that a recovery rate of 96.8–109 % has been attained for the detection of benzoate in beverages.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-10DOI: 10.1016/j.microc.2024.111607
Jiachen Zhu, Weiliang Wang, Gang Chen, Tian Gao, Zhiyong Gao, Liang Peng, Luwen Wang, Wei Cai
Uric acid (UA) and dopamine (DA) are crucial biomarkers in the human body. However, there is limited research on detecting UA or DA in subcutaneous interstitial fluid (SISF), and no studies on their simultaneous detection in SISF. Here, we developed an electrochemical microneedle sensor based on the carboxylated carbon nanotubes/multiwalled carbon nanotubes (CCNT/CNT) composite electrode for the simultaneous detection of UA and DA. Highly carboxylated CCNT was prepared and mixed with CNT and organosilicon-modified acrylic resin to form the CCNT/CNT composite. The carboxyl functional groups of CCNT enhance the electrode’s adsorption of UA and DA, while CNT improves electron conduction. The sensor achieves a wide linear detection range for UA (5–600 μM) with high sensitivity (7.13 μA μM cm) and linearity (R = 0.994), as well as a wide linear range for DA (2–200 μM) with high sensitivity (13.31 μA μM cm) and linearity (R = 0.994). The sensor achieved simultaneous detection of DA and UA in artificial interstitial fluid (ISF), with sensitivity and linearity remaining nearly unchanged compared to that in PBS, and is not affected by high levels of ascorbic acid (AA). Finally, the sensor successfully detected changes in UA levels in ISF of subjects before and after alcohol consumption , demonstrating its practical application capabilities. This study presents a practical strategy for detecting human biomarkers characterized by low cost, easy fabrication and excellent performance of electrode materials, and a well-designed microneedle sensor preparation scheme, making it highly promising for further research extension.
尿酸（UA）和多巴胺（DA）是人体内重要的生物标志物。然而，在皮下间质（SISF）中检测尿酸或多巴胺的研究非常有限，也没有在 SISF 中同时检测这两种物质的研究。在此，我们开发了一种基于羧基碳纳米管/多壁碳纳米管（CCNT/CNT）复合电极的电化学微针传感器，用于同时检测 UA 和 DA。制备了高羧基化 CCNT，并将其与 CNT 和有机硅改性丙烯酸树脂混合形成 CCNT/CNT 复合材料。CCNT 的羧基官能团增强了电极对 UA 和 DA 的吸附，而 CNT 则改善了电子传导。该传感器实现了 UA（5-600 μM）的宽线性检测范围、高灵敏度（7.13 μA μM cm）和线性度（R = 0.994），以及 DA（2-200 μM）的宽线性检测范围、高灵敏度（13.31 μA μM cm）和线性度（R = 0.994）。该传感器可同时检测人工间质液（ISF）中的 DA 和 UA，灵敏度和线性度与 PBS 中的几乎没有变化，并且不受高浓度抗坏血酸（AA）的影响。最后，该传感器成功检测出了饮酒前后受试者 ISF 中 UA 含量的变化，证明了其实际应用能力。这项研究提出了一种检测人体生物标志物的实用策略，其特点是成本低、电极材料易于制造且性能优良，同时还设计了一种精心制作的微针传感器方案，因此非常有希望进一步推广研究。
{"title":"A high-performance wearable microneedle sensor based on a carboxylated carbon nanotube-carbon nanotube composite electrode for the simultaneous detection of uric acid and dopamine","authors":"Jiachen Zhu, Weiliang Wang, Gang Chen, Tian Gao, Zhiyong Gao, Liang Peng, Luwen Wang, Wei Cai","doi":"10.1016/j.microc.2024.111607","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111607","url":null,"abstract":"Uric acid (UA) and dopamine (DA) are crucial biomarkers in the human body. However, there is limited research on detecting UA or DA in subcutaneous interstitial fluid (SISF), and no studies on their simultaneous detection in SISF. Here, we developed an electrochemical microneedle sensor based on the carboxylated carbon nanotubes/multiwalled carbon nanotubes (CCNT/CNT) composite electrode for the simultaneous detection of UA and DA. Highly carboxylated CCNT was prepared and mixed with CNT and organosilicon-modified acrylic resin to form the CCNT/CNT composite. The carboxyl functional groups of CCNT enhance the electrode’s adsorption of UA and DA, while CNT improves electron conduction. The sensor achieves a wide linear detection range for UA (5–600 μM) with high sensitivity (7.13 μA μM cm) and linearity (R = 0.994), as well as a wide linear range for DA (2–200 μM) with high sensitivity (13.31 μA μM cm) and linearity (R = 0.994). The sensor achieved simultaneous detection of DA and UA in artificial interstitial fluid (ISF), with sensitivity and linearity remaining nearly unchanged compared to that in PBS, and is not affected by high levels of ascorbic acid (AA). Finally, the sensor successfully detected changes in UA levels in ISF of subjects before and after alcohol consumption , demonstrating its practical application capabilities. This study presents a practical strategy for detecting human biomarkers characterized by low cost, easy fabrication and excellent performance of electrode materials, and a well-designed microneedle sensor preparation scheme, making it highly promising for further research extension.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142226833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-08DOI: 10.1016/j.microc.2024.111589
Alia Colniță, Daniel Marconi, Vlad Alexandru Toma, Ioana-Andreea Brezeștean, Maria Suciu, Alexandra Ciorîță, Nicoleta Elena Dina
Rapid, direct, and sensitive detection of metabolites or clinically relevant biomarkers in complex biological samples is still challenging. We report a label-free surface-enhanced Raman scattering (SERS)-based approach for highly sensitive detection of dopamine (DA) in artificial cerebrospinal fluid (aCSF) and mouse brain tissue samples. The hybrid SERS-active sensing platform was designed to maximize the hot spot distribution. It encompasses a network of flexible and polymeric nanotrenches and nanogaps fabricated by nanoimprint lithography (NIL) covered by thin films of zinc oxide (ZnO) and silver (Ag) deposited using pulsed laser deposition (PLD) and direct current magnetron sputtering (DC-MS), respectively. The growth of the ZnO and Ag thin films was assessed by scanning electron microscopy (SEM) technique. The proposed SERS substrate benefits from the interlayered semiconductor–metal contribution in SERS enhancement, leading to a versatile sensing platform with improved detection sensitivity through the increased hot spots distribution. Using this strategy, the proposed assay can achieve an ultralow DA determination at the nM level, a short testing time (<30 min), and high signal reproducibility (RSD 11 %). We also assessed the sensing attributes of our developed SERS platform to detect DA in spiked aCSF samples, and a limit of detection (LOD) of 10 μM was achieved. Additionally, after inducing Parkinson’s disease (PD) in mice, relevant biological samples (striatum and cortical brain tissue) were investigated by SERS and ELISA. Given its good stability and accuracy in complex samples, the SERS-ELISA analysis has great potential to be a powerful tool for the reliable detection of DA in remote conditions.
在复杂的生物样品中快速、直接、灵敏地检测代谢物或临床相关生物标记物仍然是一项挑战。我们报告了一种基于无标记表面增强拉曼散射（SERS）的方法，用于高灵敏度地检测人工脑脊液（aCSF）和小鼠脑组织样本中的多巴胺（DA）。混合 SERS 有源传感平台的设计旨在最大限度地扩大热点分布。它由柔性聚合物纳米沟槽和纳米缝隙网络组成，纳米沟槽和纳米缝隙是通过纳米压印光刻技术（NIL）制造的，其上覆盖着分别使用脉冲激光沉积（PLD）和直流磁控溅射（DC-MS）沉积的氧化锌（ZnO）和银（Ag）薄膜。氧化锌和银薄膜的生长情况通过扫描电子显微镜（SEM）技术进行了评估。所提议的 SERS 基底得益于半导体-金属互层对 SERS 增强的贡献，从而形成了一种多功能传感平台，并通过增加热点分布提高了检测灵敏度。利用这种策略，所提出的检测方法可以实现 nM 级的超低 DA 检测、较短的检测时间（<30 分钟）和较高的信号重现性（RSD 11%）。我们还评估了所开发的 SERS 平台在检测加标脑脊液样品中 DA 的传感属性，其检测限（LOD）达到了 10 μM。此外，在诱导小鼠患帕金森病（PD）后，我们还利用 SERS 和 ELISA 检测了相关的生物样本（纹状体和大脑皮层组织）。鉴于其在复杂样品中良好的稳定性和准确性，SERS-ELISA 分析极有可能成为在远程条件下可靠检测 DA 的有力工具。
{"title":"SERS detection of dopamine in artificial cerebrospinal fluid and in Parkinson’s disease-induced mouse cortex using a hybrid ZnO@Ag nanostructured platform","authors":"Alia Colniță, Daniel Marconi, Vlad Alexandru Toma, Ioana-Andreea Brezeștean, Maria Suciu, Alexandra Ciorîță, Nicoleta Elena Dina","doi":"10.1016/j.microc.2024.111589","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111589","url":null,"abstract":"Rapid, direct, and sensitive detection of metabolites or clinically relevant biomarkers in complex biological samples is still challenging. We report a label-free surface-enhanced Raman scattering (SERS)-based approach for highly sensitive detection of dopamine (DA) in artificial cerebrospinal fluid (aCSF) and mouse brain tissue samples. The hybrid SERS-active sensing platform was designed to maximize the hot spot distribution. It encompasses a network of flexible and polymeric nanotrenches and nanogaps fabricated by nanoimprint lithography (NIL) covered by thin films of zinc oxide (ZnO) and silver (Ag) deposited using pulsed laser deposition (PLD) and direct current magnetron sputtering (DC-MS), respectively. The growth of the ZnO and Ag thin films was assessed by scanning electron microscopy (SEM) technique. The proposed SERS substrate benefits from the interlayered semiconductor–metal contribution in SERS enhancement, leading to a versatile sensing platform with improved detection sensitivity through the increased hot spots distribution. Using this strategy, the proposed assay can achieve an ultralow DA determination at the nM level, a short testing time (<30 min), and high signal reproducibility (RSD 11 %). We also assessed the sensing attributes of our developed SERS platform to detect DA in spiked aCSF samples, and a limit of detection (LOD) of 10 μM was achieved. Additionally, after inducing Parkinson’s disease (PD) in mice, relevant biological samples (striatum and cortical brain tissue) were investigated by SERS and ELISA. Given its good stability and accuracy in complex samples, the SERS-ELISA analysis has great potential to be a powerful tool for the reliable detection of DA in remote conditions.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-07DOI: 10.1016/j.microc.2024.111577
Fuat Aslan, Haluk Bingol, Ali Tor
Herein, amino functionalized Zr-based metal organic framework (UiO-66-NH) on carbon cloth substrate was synthesized by using one-step hot-pressing method and the product (UiO-NH@CC) was evaluated as a new solid phase extraction (SPE) material for determination and speciation of inorganic arsenic species (As(V) and As(III)) in water samples through energy dispersive X-ray fluorescence (EDXRF) spectrometry. SPE efficiency of UiO-NH@CC was examined in batch and continuous adsorption (pre-concentration) processes and combination of either batch or continuous pre-concentration process with EDXRF spectrometry proposed analytical method, offering remarkable benefits (i.e., user-friendly, no elution procedure, environmentally friendly and cost-effective, etc.). Batch adsorption studies indicated that As(V) could be selectively adsorbed by UiO-NH@CC at pH 2 and better represented by Freundlich isotherm model in 60 min of equilibrium time. Continuous adsorption studies at pH 2 demonstrated a drop in As(V) adsorption efficiency of UiO-NH@CC for sample loading volume above 30 mL with increasing flow rate from 0.3 to 1.0 mL/min and initial As(V) concentration from 20 to 100 µg/L. By considering fortification experiments on real water samples at two levels (5 and 50 µg/L) of As(V) species, both batch and continuous pre-concentration steps coupled with EDXRF spectrometry, with LOD values of 0.790 and 0.220 µg/L, respectively, indicated good recoveries in the range of 87(±11)–104(±7) with no statistically significant differences. Finally, the continuous pre-concentration step followed by EDXRF measurement, as a representative analytical method, was of successful applicability to the speciation of As(III) and As(V) in real water samples with notable advantages (i.e., effortless, economical, practical and reproducible, etc.).
{"title":"One-step hot-press synthesis of amino functionalized Zr-based metal organic framework for determination and speciation of trace inorganic arsenic species in water samples with EDXRF spectrometry","authors":"Fuat Aslan, Haluk Bingol, Ali Tor","doi":"10.1016/j.microc.2024.111577","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111577","url":null,"abstract":"Herein, amino functionalized Zr-based metal organic framework (UiO-66-NH) on carbon cloth substrate was synthesized by using one-step hot-pressing method and the product (UiO-NH@CC) was evaluated as a new solid phase extraction (SPE) material for determination and speciation of inorganic arsenic species (As(V) and As(III)) in water samples through energy dispersive X-ray fluorescence (EDXRF) spectrometry. SPE efficiency of UiO-NH@CC was examined in batch and continuous adsorption (pre-concentration) processes and combination of either batch or continuous pre-concentration process with EDXRF spectrometry proposed analytical method, offering remarkable benefits (i.e., user-friendly, no elution procedure, environmentally friendly and cost-effective, etc.). Batch adsorption studies indicated that As(V) could be selectively adsorbed by UiO-NH@CC at pH 2 and better represented by Freundlich isotherm model in 60 min of equilibrium time. Continuous adsorption studies at pH 2 demonstrated a drop in As(V) adsorption efficiency of UiO-NH@CC for sample loading volume above 30 mL with increasing flow rate from 0.3 to 1.0 mL/min and initial As(V) concentration from 20 to 100 µg/L. By considering fortification experiments on real water samples at two levels (5 and 50 µg/L) of As(V) species, both batch and continuous pre-concentration steps coupled with EDXRF spectrometry, with LOD values of 0.790 and 0.220 µg/L, respectively, indicated good recoveries in the range of 87(±11)–104(±7) with no statistically significant differences. Finally, the continuous pre-concentration step followed by EDXRF measurement, as a representative analytical method, was of successful applicability to the speciation of As(III) and As(V) in real water samples with notable advantages (i.e., effortless, economical, practical and reproducible, etc.).","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-07DOI: 10.1016/j.microc.2024.111580
Mehmet Aslan, Abdulkadir Levent
Ribociclib (RIB) is a cyclin-dependent kinase inhibitor used in the treatment of breast cancer. In this study, for the first time, the electrochemical behavior, quantification and interaction of RIB with DNA were carried out using voltammetric, spectrophotometric and molecular docking techniques. An environmentally-friendly disposable pencil graphite electrode (PGE) sensor was used as the working electrode. Utilizing the cyclic voltammetry technique, RIB produced an irreversible anodic wave around +0.837 V and reversible signals around +0.278 V/+0.209 V on the PGE surface. Using the PGE sensor, the RIB compound gave a very distinct anodic signal at a potential of +0.77 V in PBS (pH 3.0). For this analytical signal, the limit of detection and limit of quantitation values of RIB in the concentration range of 0.0139 3 M–0.0973 3 M in PBS (pH 3.0) were determined as 2.84 nM and 9.46 nM, respectively. The interaction of RIB with DNA was studied by voltammetric, spectrophotometric and molecular docking techniques. In the evaluation of the results obtained with all three techniques, the interaction of the RIB molecule with DNA was determined to occur through minor groove binding.
Ribociclib（RIB）是一种用于治疗乳腺癌的细胞周期蛋白依赖性激酶抑制剂。本研究首次采用伏安法、分光光度法和分子对接技术对 RIB 与 DNA 的电化学行为、定量和相互作用进行了研究。研究使用了环保型一次性铅笔石墨电极（PGE）传感器作为工作电极。利用循环伏安技术，RIB 在 PGE 表面产生了 +0.837 V 左右的不可逆阳极波和 +0.278 V/+0.209 V 左右的可逆信号。使用 PGE 传感器，RIB 化合物在 PBS（pH 值 3.0）中的电位为 +0.77 V 时会产生非常明显的阳极信号。对于该分析信号，在 PBS（pH 3.0）中 0.0139 3 M-0.0973 3 M 的浓度范围内，RIB 的检出限和定量限分别为 2.84 nM 和 9.46 nM。通过伏安法、分光光度法和分子对接技术研究了 RIB 与 DNA 的相互作用。在对所有三种技术得出的结果进行评估后，确定 RIB 分子与 DNA 的相互作用是通过小沟结合发生的。
{"title":"First voltammetric studies, spectrophotometric and molecular docking investigations of the interaction of an anticancer drug ribociclib-DNA and analytical applications of disposable pencil graphite sensor","authors":"Mehmet Aslan, Abdulkadir Levent","doi":"10.1016/j.microc.2024.111580","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111580","url":null,"abstract":"Ribociclib (RIB) is a cyclin-dependent kinase inhibitor used in the treatment of breast cancer. In this study, for the first time, the electrochemical behavior, quantification and interaction of RIB with DNA were carried out using voltammetric, spectrophotometric and molecular docking techniques. An environmentally-friendly disposable pencil graphite electrode (PGE) sensor was used as the working electrode. Utilizing the cyclic voltammetry technique, RIB produced an irreversible anodic wave around +0.837 V and reversible signals around +0.278 V/+0.209 V on the PGE surface. Using the PGE sensor, the RIB compound gave a very distinct anodic signal at a potential of +0.77 V in PBS (pH 3.0). For this analytical signal, the limit of detection and limit of quantitation values of RIB in the concentration range of 0.0139 3 M–0.0973 3 M in PBS (pH 3.0) were determined as 2.84 nM and 9.46 nM, respectively. The interaction of RIB with DNA was studied by voltammetric, spectrophotometric and molecular docking techniques. In the evaluation of the results obtained with all three techniques, the interaction of the RIB molecule with DNA was determined to occur through minor groove binding.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-07DOI: 10.1016/j.microc.2024.111606
Taimei Cai, Feifan Liu, Jianwen Tian, Tao Wen, Hailong Peng
Glutathione (GSH) is an essential natural antioxidant, and plays a crucial role in the prevention of various diseases. Herein, a nitrogen-doped polydopamine manganese nanoparticle (N-PDA@Mn) was prepared and used as nanozyme sensor for the rapid and colorimetric detection of GSH. N-PDA@Mn demonstrated excellent oxidase-like activity ( = 0.0841 mM and = 3.6 × 10 MS), exhibiting a rapid balance period of < 100 s. N-PDA@Mn could activate dissolved oxygen to primarily generate singlet-oxygen (O), which oxidized colorless 3,3′, 5, 5′-tetramethylbenzidine (TMB) to blue oxidized (ox-TMB). Because GSH could rapidly deplete O, an ultrafast and colorimetric strategy was developed for GSH detection based on N-PDA@Mn. The GSH detection range was 0.5–100 μM, and the detection limit was 0.28 µM. Importantly, at room temperature, the detection time was only 30 s, which was short than that of previously reported GSH detection methods. Furthermore, a point-of-care testing (POCT) platform was designed by combining the N-PDA@Mn colorimetric sensor with a smartphone. Use the POCT platform, a broad linear detection range of 0.5–100 μM and a detection limit of 0.40 μM were obtained. Subsequently, the N-PDA@Mn colorimetric sensor and POCT platform were successfully used in the real-time and on-site quantification of GSH in human serum, achieving a high accuracy and good recovery. Therefore, the N-PDA@Mn-based methods could realize the rapid, on-site visualization, and economical detection of GSH, demonstrating their substantial potential for application in practical testing in fields of food and medicine.
{"title":"Nitrogen-doped polydopamine manganese nanoparticles with excellent oxidase-like activity for ultrafast detection of glutathione at room temperature","authors":"Taimei Cai, Feifan Liu, Jianwen Tian, Tao Wen, Hailong Peng","doi":"10.1016/j.microc.2024.111606","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111606","url":null,"abstract":"Glutathione (GSH) is an essential natural antioxidant, and plays a crucial role in the prevention of various diseases. Herein, a nitrogen-doped polydopamine manganese nanoparticle (N-PDA@Mn) was prepared and used as nanozyme sensor for the rapid and colorimetric detection of GSH. N-PDA@Mn demonstrated excellent oxidase-like activity ( = 0.0841 mM and = 3.6 × 10 MS), exhibiting a rapid balance period of < 100 s. N-PDA@Mn could activate dissolved oxygen to primarily generate singlet-oxygen (O), which oxidized colorless 3,3′, 5, 5′-tetramethylbenzidine (TMB) to blue oxidized (ox-TMB). Because GSH could rapidly deplete O, an ultrafast and colorimetric strategy was developed for GSH detection based on N-PDA@Mn. The GSH detection range was 0.5–100 μM, and the detection limit was 0.28 µM. Importantly, at room temperature, the detection time was only 30 s, which was short than that of previously reported GSH detection methods. Furthermore, a point-of-care testing (POCT) platform was designed by combining the N-PDA@Mn colorimetric sensor with a smartphone. Use the POCT platform, a broad linear detection range of 0.5–100 μM and a detection limit of 0.40 μM were obtained. Subsequently, the N-PDA@Mn colorimetric sensor and POCT platform were successfully used in the real-time and on-site quantification of GSH in human serum, achieving a high accuracy and good recovery. Therefore, the N-PDA@Mn-based methods could realize the rapid, on-site visualization, and economical detection of GSH, demonstrating their substantial potential for application in practical testing in fields of food and medicine.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we develop a novel ratiometric fluorescent nanosensor for the detection excess Co in soil. This fluorescent nanosensor is fabricated using mesoporous silica-encapsulated nitrogen-doped carbon dots (N-CDs@mSiO) and gold nanoclusters stabilized by bovine serum albumin (BSA-AuNCs). The green fluorescence of the carbon dots within the mesoporous silica spheres (mSiO) serves as an internal reference signal. The red fluoresence BSA-AuNCs are covalently connected onto the surface of amino-functionalized nanospheres (N-CDs@mSiO-NH), providing the response signal. This nanosensor has dual emission peaks at 520 nm and 650 nm under excitation wavelength of 380 nm. The addition of Co to this nanosensor causes the fluorescence quenching at 650 nm while the green fluorescence at 520 nm remains unchanged, resulting in fluorescence color change from yellow to green. The developed ratiometric fluorescence nanosensor exhibits excellent selectivity to Co with a range of 2.00–200.00 μM and a detection limit as low as 0.74 μM. In addition, a Co prediction model is developed using the Light Gradient Boosting Machine (LightGBM) algorithm. The entire detection process is within 10 s and the model prediction value is as high as 0.991 on average. This shows that the proposed fluorescent nanosensor, optimized with the LightGBM algorithm, provides an efficient, environmentally friendly and potentially practical solution for Co detection.
在本研究中，我们开发了一种新型比率荧光纳米传感器，用于检测土壤中过量的钴。这种荧光纳米传感器是利用介孔二氧化硅封装的掺氮碳点（N-CDs@mSiO）和牛血清白蛋白稳定的金纳米团簇（BSA-AuNCs）制成的。介孔二氧化硅球（mSiO）中碳点的绿色荧光可作为内部参考信号。红色荧光 BSA-AuNCs 与氨基功能化纳米球（N-CDs@mSiO-NH）表面共价连接，提供响应信号。在 380 nm 的激发波长下，该纳米传感器在 520 nm 和 650 nm 处具有双发射峰。向该纳米传感器中添加 Co 会导致 650 nm 处的荧光淬灭，而 520 nm 处的绿色荧光保持不变，从而使荧光颜色从黄色变为绿色。所开发的比率荧光纳米传感器对 Co 具有极佳的选择性，其选择范围为 2.00-200.00 μM，检测限低至 0.74 μM。此外，还利用光梯度提升机（LightGBM）算法建立了一个 Co 预测模型。整个检测过程在 10 秒内完成，模型预测值平均高达 0.991。这表明，利用 LightGBM 算法优化的拟议荧光纳米传感器为 Co 检测提供了一种高效、环保且具有潜在实用性的解决方案。
{"title":"Gold nanoclusters and amino-modified mesoporous silica-encapsulated carbon dot fluorescence nanosensors combined with LightGBM algorithm for ultra-fast detection of Co2+","authors":"Chenyue Kang, Yueying Li, Xuran Wang, Yan Zhang, Caihong Zhang, Ying Zhou, Shaomin Shuang, Guomei Zhang","doi":"10.1016/j.microc.2024.111610","DOIUrl":"https://doi.org/10.1016/j.microc.2024.111610","url":null,"abstract":"In this study, we develop a novel ratiometric fluorescent nanosensor for the detection excess Co in soil. This fluorescent nanosensor is fabricated using mesoporous silica-encapsulated nitrogen-doped carbon dots (N-CDs@mSiO) and gold nanoclusters stabilized by bovine serum albumin (BSA-AuNCs). The green fluorescence of the carbon dots within the mesoporous silica spheres (mSiO) serves as an internal reference signal. The red fluoresence BSA-AuNCs are covalently connected onto the surface of amino-functionalized nanospheres (N-CDs@mSiO-NH), providing the response signal. This nanosensor has dual emission peaks at 520 nm and 650 nm under excitation wavelength of 380 nm. The addition of Co to this nanosensor causes the fluorescence quenching at 650 nm while the green fluorescence at 520 nm remains unchanged, resulting in fluorescence color change from yellow to green. The developed ratiometric fluorescence nanosensor exhibits excellent selectivity to Co with a range of 2.00–200.00 μM and a detection limit as low as 0.74 μM. In addition, a Co prediction model is developed using the Light Gradient Boosting Machine (LightGBM) algorithm. The entire detection process is within 10 s and the model prediction value is as high as 0.991 on average. This shows that the proposed fluorescent nanosensor, optimized with the LightGBM algorithm, provides an efficient, environmentally friendly and potentially practical solution for Co detection.","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":null,"pages":null},"PeriodicalIF":4.8,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142212480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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