Pub Date : 2024-11-29DOI: 10.1016/j.microc.2024.112298
Farah Al-Hammashi, Fariborz Momenbeik
In this study, we employed microextraction by packed sorbent (MEPS) using polycatechol-coated cigarette filters (PCCFs) as a sorbent to extract acidic non-steroidal anti-inflammatory drugs (NSAIDs) from water samples, followed by high-performance liquid chromatographic analysis. The formation of coating on cigarette filter was confirmed by Fourier transform infrared (FT-IR) spectroscopy, UV–vis diffuse reflectance spectroscopy (DRS) and scanning electron microscopy (SEM). The effect of coating parameters on extraction efficiency of acidic NASIDs were investigated and 106.0 mg of Na2CO3, 30.0 mg of catechol and 3 h coating time were selected as optimum conditions. Additionally, other factors affecting the analyte extraction efficiency were optimized including a sample solution pH of 2.0, 700.0 µL of methanol as the elution solvent, 3 sorption cycles, 2 desorption cycles, and a 1.5 ml sample volume. Under the optimum conditions the calibration curves for all three analytes were draw and the analytical performance parameters including linear dynamic range (50.0–1000.0 µg.L−1 for naproxen and diclofenac and 100.0–500.0 µg.L−1 for ibuprofen) with R2 > 0.9982, limit of detection (LOD < 45.3 µg.L−1), precision (RSD% < 6.5), accuracy (recovery% > 90.1) and enrichment factor (EF > 2.7) were evaluated. Finally, this method was successfully used to extract and determine the amount of selected acidic NSAIDs in wastewater samples. The sorbent was cheap, easily prepared, efficient, and even reusable.
{"title":"Polycatechol coated cigarette filter as a sorbent for microextraction by packed sorbent of acidic non-steroidal anti-inflammatory drugs (NSAIDs) from wastewater samples","authors":"Farah Al-Hammashi, Fariborz Momenbeik","doi":"10.1016/j.microc.2024.112298","DOIUrl":"10.1016/j.microc.2024.112298","url":null,"abstract":"<div><div>In this study, we employed microextraction by packed sorbent (MEPS) using polycatechol-coated cigarette filters (PCCFs) as a sorbent to extract acidic non-steroidal anti-inflammatory drugs (NSAIDs) from water samples, followed by high-performance liquid chromatographic analysis. The formation of coating on cigarette filter was confirmed by Fourier transform infrared (FT-IR) spectroscopy, UV–vis diffuse reflectance spectroscopy (DRS) and scanning electron microscopy (SEM). The effect of coating parameters on extraction efficiency of acidic NASIDs were investigated and 106.0 mg of Na<sub>2</sub>CO<sub>3</sub>, 30.0 mg of catechol and 3 h coating time were selected as optimum conditions. Additionally, other factors affecting the analyte extraction efficiency were optimized including a sample solution pH of 2.0, 700.0 µL of methanol as the elution solvent, 3 sorption cycles, 2 desorption cycles, and a 1.5 ml sample volume. Under the optimum conditions the calibration curves for all three analytes were draw and the analytical performance parameters including linear dynamic range (50.0–1000.0 µg.L<sup>−1</sup> for naproxen and diclofenac and 100.0–500.0 µg.L<sup>−1</sup> for ibuprofen) with R<sup>2</sup> > 0.9982, limit of detection (LOD < 45.3 µg.L<sup>−1</sup>), precision (RSD% < 6.5), accuracy (recovery% > 90.1) and enrichment factor (EF > 2.7) were evaluated. Finally, this method was successfully used to extract and determine the amount of selected acidic NSAIDs in wastewater samples. The sorbent was cheap, easily prepared, efficient, and even reusable.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112298"},"PeriodicalIF":4.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, we focused on a novel agricultural waste material, specifically a plant known as Nicotiana glauca Graham (NgG), which is highly abundant in Morocco. The investigation involved testing four activating agents H3PO4, H2SO4, NaOH, and ZnCl2 on Nicotiana glauca Graham (NgG) to assess their effects. By employing an experimental design, we successfully determined the optimal conditions for this activation process. The resulting activated carbon was then evaluated for its effectiveness in removing two phenolic pollutants, Bisphenol A (BPA) and β-naphthol (BNL). Analysis using FTIR revealed various functional groups on the activated carbon surface, including P = O, P-O-C aromatics, and O-H groups, which played a crucial role in the adsorption of BPA and BNL. XRD analysis indicated that the optimal adsorbent was amorphous, while Zeta potential measurements showed a significant decrease in pollutant removal rates after reaching a pH level of nearly 10. The activated carbon produced from H3PO4 exhibited a surface area of 1078 m2/g. Experimental adsorption results at the highest removal rate showed qBPAs = 125.82 mg/g for BPA and qBNLs = 62.82 mg/g for BNL in individual mode, while in the mixed mode, qBPAm = 16.22 mg/g for BPA and qBNLm = 16.04 mg/g for BNL were observed. To enhance our study, we utilized Density Functional Theory (DFT) calculations to identify the most electrophilic and nucleophilic regions on BPA and BNL. The analysis highlighted the hydroxyl groups (–OH) of BNL and BPA as the most significant negative zones, crucial for understanding the underlying mechanisms and explaining the experimental observations. In the isotherm analysis, we identified the Temkin model in a singular mode and the Langmuir model in a mixture mode, showcasing a notable distinction between the two modes.
{"title":"Competitive adsorption of two phenolic pollutants compounds using a novel biosorbent: Analytics (HPLC), Statistical (experimental design), and theoretical studies (DFT)","authors":"Taoufiq Bouzid , Aicha Naboulsi , Abdelali Grich , Hicham Yazid , Julien Vieillard , Abdelmajid Regti , Mamoune El Himri , Mohammadine El Haddad","doi":"10.1016/j.microc.2024.112281","DOIUrl":"10.1016/j.microc.2024.112281","url":null,"abstract":"<div><div>In this study, we focused on a novel agricultural waste material, specifically a plant known as Nicotiana glauca Graham (NgG), which is highly abundant in Morocco. The investigation involved testing four activating agents H<sub>3</sub>PO<sub>4</sub>, H<sub>2</sub>SO<sub>4</sub>, NaOH, and ZnCl<sub>2</sub> on Nicotiana glauca Graham (NgG) to assess their effects. By employing an experimental design, we successfully determined the optimal conditions for this activation process. The resulting activated carbon was then evaluated for its effectiveness in removing two phenolic pollutants, Bisphenol A (BPA) and β-naphthol (BNL). Analysis using FTIR revealed various functional groups on the activated carbon surface, including P = O, P-O-C aromatics, and O-H groups, which played a crucial role in the adsorption of BPA and BNL. XRD analysis indicated that the optimal adsorbent was amorphous, while Zeta potential measurements showed a significant decrease in pollutant removal rates after reaching a pH level of nearly 10. The activated carbon produced from H<sub>3</sub>PO<sub>4</sub> exhibited a surface area of 1078 m<sup>2</sup>/g. Experimental adsorption results at the highest removal rate showed q<sub>BPAs</sub> = 125.82 mg/g for BPA and q<sub>BNLs</sub> = 62.82 mg/g for BNL in individual mode, while in the mixed mode, q<sub>BPAm</sub> = 16.22 mg/g for BPA and q<sub>BNLm</sub> = 16.04 mg/g for BNL were observed. To enhance our study, we utilized Density Functional Theory (DFT) calculations to identify the most electrophilic and nucleophilic regions on BPA and BNL. The analysis highlighted the hydroxyl groups (–OH) of BNL and BPA as the most significant negative zones, crucial for understanding the underlying mechanisms and explaining the experimental observations. In the isotherm analysis, we identified the Temkin model in a singular mode and the Langmuir model in a mixture mode, showcasing a notable distinction between the two modes.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112281"},"PeriodicalIF":4.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1016/j.microc.2024.112284
Kalyani A. Birari , Pravin O. Patil , Shadma Wahab , Mohammad Khalid , Mahendra Mahajan , Rahul S. Tade , Sanjaykumar B. Bari , Zamir G. Khan
Niclosamide (NIC), a widely recognized antiparasitic and anthelmintic agent, plays a critical role in aquaculture and livestock breeding, particularly in controlling parasitic growth and schistosomiasis, endorsed by the WHO as an essential molluscicide. However, excessive use has led to NIC residues in animal products and environmental sources, posing significant health risks. The detection of NIC residues in food products has thus become essential to safeguarding public health. This review provides a detailed overview of NIC regulatory residue limits and the associated health risks of NIC residue in food sources. In addition, it delves into various established detection methods, critically evaluating their strengths and limitations. The evaluation seeks to improve food product safety and monitoring by emphasising important methodologies and suggesting improvements in detection efficiency and accuracy. Lastly, future perspectives for the advancement of sophisticated analytical techniques are examined, providing information on how NIC detection techniques may change in response to escalating safety requirements.
{"title":"A comprehensive review on niclosamide detection in foodstuffs and pharmaceutical preparations: Diverse analytical approaches and emerging techniques","authors":"Kalyani A. Birari , Pravin O. Patil , Shadma Wahab , Mohammad Khalid , Mahendra Mahajan , Rahul S. Tade , Sanjaykumar B. Bari , Zamir G. Khan","doi":"10.1016/j.microc.2024.112284","DOIUrl":"10.1016/j.microc.2024.112284","url":null,"abstract":"<div><div>Niclosamide (NIC), a widely recognized antiparasitic and anthelmintic agent, plays a critical role in aquaculture and livestock breeding, particularly in controlling parasitic growth and schistosomiasis, endorsed by the WHO as an<!--> <!-->essential molluscicide. However, excessive use has led to NIC residues in animal products and environmental sources, posing significant health risks. The detection of NIC residues in food products has thus become essential to safeguarding public health. This review provides a detailed overview of NIC regulatory residue limits and the associated health risks of NIC residue in food sources. In addition, it delves into various established detection methods, critically evaluating their strengths and limitations. The evaluation seeks to improve food product safety and monitoring by emphasising important methodologies and suggesting improvements in detection efficiency and accuracy. Lastly, future perspectives for the advancement of sophisticated analytical techniques are examined, providing information on how NIC detection techniques may change in response to escalating safety requirements.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112284"},"PeriodicalIF":4.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1016/j.microc.2024.112287
Yanying Li , Wei Guan , Yuqing Wang , Zhijiang Chen , Peng Jiang , Ye Sun , Zhichao Hao , Qingshan Chen , Lili Zhang , Bingyou Yang , Yan Liu
In this study, a comprehensive data filtering and identification strategy was developed. The five-point mass defect filtering (MDF) screening was combined with characteristic fragments determined based on representative reference standards and literature to identify quinoline alkaloids and limonoids in the root bark of Dictamnus dasycarpus Turcz. (DD) extract. Compared with typical characterization method, by using the established five-point MDF model, a large number of background interference ions were filtered out with an efficiency of 82 % (from 6280 to 1136). It’s the first time to construct a characteristic fragments database based on six substructures of quinoline alkaloids and limonoids in DD. Finally, by using CLogP values and dipole moments to determine the elution order of isomers, a total of 113 compounds were identified efficiently and accurately, including 95 quinoline alkaloids and 18 limonoids. Additionally, 33 compounds from DD were identified for the first time, and 23 were regarded as potential novel compounds for the first time (7 furoquinoline alkaloids, 11 quinolinone alkaloids, 1 quinolone, 1 A.D-ring open-loop limonoid-1, and 3 A.D-ring open-loop limonoids-2). Untargeted metabolomics combined with machine learning-based chemometrics was applied to analyze the metabolite profiles of 72 batches of DD from 12 production regions. Finally, a total of 27 differential metabolites (21 quinoline alkaloids and 6 limonoids) were identified by the PLS-DA analysis. The results indicated that this method was an efficient, accurate, and promising approach for classifying and exploring compounds in the complex system of natural products, providing a basis for evaluating the quality of DD from different sources.
{"title":"Strategy of mass defect filter combined with characteristic fragment analysis for the chemical profiling of Dictamnus dasycarpus Turcz. From multiple regions","authors":"Yanying Li , Wei Guan , Yuqing Wang , Zhijiang Chen , Peng Jiang , Ye Sun , Zhichao Hao , Qingshan Chen , Lili Zhang , Bingyou Yang , Yan Liu","doi":"10.1016/j.microc.2024.112287","DOIUrl":"10.1016/j.microc.2024.112287","url":null,"abstract":"<div><div>In this study, a comprehensive data filtering and identification strategy was developed. The five-point mass defect filtering (MDF) screening was combined with characteristic fragments determined based on representative reference standards and literature to identify quinoline alkaloids and limonoids in the root bark of Dictamnus dasycarpus Turcz. (DD) extract. Compared with typical characterization method, by using the established five-point MDF model, a large number of background interference ions were filtered out with an efficiency of 82 % (from 6280 to 1136). It’s the first time to construct a characteristic fragments database based on six substructures of quinoline alkaloids and limonoids in DD. Finally, by using CLogP values and dipole moments to determine the elution order of isomers, a total of 113 compounds were identified efficiently and accurately, including 95 quinoline alkaloids and 18 limonoids. Additionally, 33 compounds from DD were identified for the first time, and 23 were regarded as potential novel compounds for the first time (7 furoquinoline alkaloids, 11 quinolinone alkaloids, 1 quinolone, 1 A.D-ring open-loop limonoid-1, and 3 A.D-ring open-loop limonoids-2). Untargeted metabolomics combined with machine learning-based chemometrics was applied to analyze the metabolite profiles of 72 batches of DD from 12 production regions. Finally, a total of 27 differential metabolites (21 quinoline alkaloids and 6 limonoids) were identified by the PLS-DA analysis. The results indicated that this method was an efficient, accurate, and promising approach for classifying and exploring compounds in the complex system of natural products, providing a basis for evaluating the quality of DD from different sources.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112287"},"PeriodicalIF":4.9,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1016/j.microc.2024.112293
Zhiruo Yang , Zhenlong Chen , Zhipeng Zhang , Huirong Xu , Nicole Jaffrezic-Renault , Junrui Hu , Zhenzhong Guo
The human pathogen Parvovirus B19 (B19V) is known to cause a spectrum of diseases. Presently, no vaccines or antiviral medications are accessible for its prevention or cure. This highlights the pressing need for the advancement of efficient and affordable diagnostic technologies in vitro, which are essential for managing and curbing the spread of infectious diseases. In our research, we have introduced an innovative nanocomposite material, g-C3N4/DWCNT-COOH/PtNPs, synthesized via a one-step hydrothermal process. This material is applied on the electrode surface to enhance the primary signal. The capture probe cDNA is affixed to the electrode via a Pt-S bond, a method that ensures a stable immobilization for detection purposes, and in conjunction with nucleic acid detection mediated by Clostridium butyricum Argonaute (CbAgo) from the mesophilic bacterium, an electrochemical Argonaute biosensor utilizing silver metallization was constructed, targeting the non-structure protein 1 (NS1) region of the virus genome. Benefiting from the lack of sequence constraints similar to the Protospacer Adjacent Motif (PAM), CbAgo can utilize an easily designed and cost-effective guide DNA (gDNA) to recognize the target sequence. By incorporating a polymerase chain reaction (PCR) pre-amplification step, along with silver metallization for secondary signal amplification and electrochemical analysis techniques, a highly sensitive and specific detection is obtained. The biosensing platform exhibits a detection range from 10-17 M to 10-12 M, with a detection limit as low as 2.62 aM. Compared to current detection methods for this virus, it demonstrates superior analytical performance and validates its effectiveness for use with real serum samples.
{"title":"Detection of parvovirus B19 genomic fragments using an electrochemical biosensor based on argonaute-assisted silver metallization","authors":"Zhiruo Yang , Zhenlong Chen , Zhipeng Zhang , Huirong Xu , Nicole Jaffrezic-Renault , Junrui Hu , Zhenzhong Guo","doi":"10.1016/j.microc.2024.112293","DOIUrl":"10.1016/j.microc.2024.112293","url":null,"abstract":"<div><div>The human pathogen Parvovirus B19 (B19V) is known to cause a spectrum of diseases. Presently, no vaccines or antiviral medications are accessible for its prevention or cure. This highlights the pressing need for the advancement of efficient and affordable diagnostic technologies <em>in vitro</em>, which are essential for managing and curbing the spread of infectious diseases. In our research, we have introduced an innovative nanocomposite material, g-C<sub>3</sub>N<sub>4</sub>/DWCNT-COOH/PtNPs, synthesized via a one-step hydrothermal process. This material is applied on the electrode surface to enhance the primary signal. The capture probe cDNA is affixed to the electrode via a Pt-S bond, a method that ensures a stable immobilization for detection purposes, and in conjunction with nucleic acid detection mediated by <em>Clostridium butyricum</em> Argonaute (<em>Cb</em>Ago) from the mesophilic bacterium, an electrochemical Argonaute biosensor utilizing silver metallization was constructed, targeting the non-structure protein 1 (NS1) region of the virus genome. Benefiting from the lack of sequence constraints similar to the Protospacer Adjacent Motif (PAM), <em>Cb</em>Ago can utilize an easily designed and cost-effective guide DNA (gDNA) to recognize the target sequence. By incorporating a polymerase chain reaction (PCR) pre-amplification step, along with silver metallization for secondary signal amplification and electrochemical analysis techniques, a highly sensitive and specific detection is obtained. The biosensing platform exhibits a detection range from 10<sup>-17</sup> M to 10<sup>-12</sup> M, with a detection limit as low as 2.62 aM. Compared to current detection methods for this virus, it demonstrates superior analytical performance and validates its effectiveness for use with real serum samples.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112293"},"PeriodicalIF":4.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Current evidence indicates that the higher nutritional value of organic foods, compared to their conventional counterparts, significantly impacts consumer demand. However, the application of thermal processes during processing may lead to a loss of nutritional values, including total phenolics and antioxidants, thereby failing to meet consumer expectations. Phenolics, known as some of the most potent natural antioxidants and are typically assessed through biochemical tests based on the concentration and reactivity of antioxidant compounds. However, the extraction methods can affect the accuracy of phenolic content measurement. In this study, the total phenolic (TP) (mg/100 g) capacity of fresh, hot air (HA)-dried, and intermittent microwave (IMW)-dried organic and conventional black carrots were compared, and the detectability of these compounds using a colorimeter and FT-NIRS was evaluated. PLSR models developed for estimating the TP content using FT-NIRS resulted in successful outcomes (IMW: R2val = 0.78, RMSEP = 51.4 mg/100 g; HA: R2val = 0.70, RMSEP = 63.0 mg/100 g). The highest prediction accuracy was achieved with drying treatments at 300 W (R2val = 0.95, RMSEP = 5.1 mg/100 g) and 450 W (R2val = 0.83, RMSEP = 50.7 mg/100 g), as well as at 80 °C (R2val = 0.90, RMSEP = 21.0 mg/100 g). The drying methods significantly influenced the TP content and the accuracy of TP prediction in samples. Additionally, organic carrots contained a higher level of total phenolic compared to conventional.
{"title":"Comparison of total phenolic content in organic and conventional carrot under different drying conditions using non-destructive analysis techniques","authors":"Aysel Arslan , Nafiz Çeliktaş , Yurtsever Soysal , Muharrem Keskin","doi":"10.1016/j.microc.2024.112279","DOIUrl":"10.1016/j.microc.2024.112279","url":null,"abstract":"<div><div>Current evidence indicates that the higher nutritional value of organic foods, compared to their conventional counterparts, significantly impacts consumer demand. However, the application of thermal processes during processing may lead to a loss of nutritional values, including total phenolics and antioxidants, thereby failing to meet consumer expectations. Phenolics, known as some of the most potent natural antioxidants and are typically assessed through biochemical tests based on the concentration and reactivity of antioxidant compounds. However, the extraction methods can affect the accuracy of phenolic content measurement. In this study, the total phenolic (TP) (mg/100 g) capacity of fresh, hot air (HA)-dried, and intermittent microwave (IMW)-dried organic and conventional black carrots were compared, and the detectability of these compounds using a colorimeter and FT-NIRS was evaluated. PLSR models developed for estimating the TP content using FT-NIRS resulted in successful outcomes (IMW: R<sup>2</sup><sub>val</sub> = 0.78, RMSEP = 51.4 mg/100 g; HA: R<sup>2</sup><sub>val</sub> = 0.70, RMSEP = 63.0 mg/100 g). The highest prediction accuracy was achieved with drying treatments at 300 W (R<sup>2</sup><sub>val</sub> = 0.95, RMSEP = 5.1 mg/100 g) and 450 W (R<sup>2</sup><sub>val</sub> = 0.83, RMSEP = 50.7 mg/100 g), as well as at 80 °C (R<sup>2</sup><sub>val</sub> = 0.90, RMSEP = 21.0 mg/100 g). The drying methods significantly influenced the TP content and the accuracy of TP prediction in samples. Additionally, organic carrots contained a higher level of total phenolic compared to conventional.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112279"},"PeriodicalIF":4.9,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27DOI: 10.1016/j.microc.2024.112301
Saliha Dinç , Tim Tjardts , Gregor Maschkowitz , Vivian Lukaszczuk , Seyed Mohammad Taghi Gharibzahedi , Zeynep Altintas
Campylobacter jejuni, a leading cause of gastroenteritis worldwide, poses significant detection challenges due to the labor-intensive and insufficiently sensitive current methods. Developing efficient, rapid diagnostics is vital for clinical and food industry applications; yet the vulnerability of biological elements in creating bacterial sensors remains a major obstacle. This study introduces an innovative fluorescence-based sensor employing fully synthetic carbon dot (CDs) functionalized molecularly imprinted polymer (CDs@nanoMIPs) receptors to detect this foodborne pathogenic bacterium. The CDs@nanoMIPs synthesis was accomplished using a solid-phase approach, with an immunodominant epitope of C. jejuni serving as a guiding template. During the polymerization process, nitrogen-doped CDs were synthesized in situ and incorporated into nanoMIPs as a fluorescent tag, constituting approximately 19 % of the composite. The synthesized nanomaterials were characterized by employing transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), fluorescence microscopy, UV–Vis spectrophotometry, and fluorescence spectrometry. CDs@nanoMIPs demonstrated a notable blue fluorescence when excited at 350 nm, along with excellent photostability and a negative zeta potential. These nanostructured particles (59 nm) exhibited moderate polydispersity and a spherical morphology. The FT-IR spectrum deviated from that of conventional CDs, aligning more closely with the template epitope. When in contact with C. jejuni, CDs@nanoMIPs induced a significant increase in fluorescence intensity, enabling the efficient bacterial detection. This interaction showed exceptional affinity and sensitivity towards C. jejuni, featuring a linear range of 1 × 101–1 × 108 CFU mL−1 (R2 = 0.98) and a low detection limit of 4.6 CFU mL−1. CDs@nanoMIPs-based C. jejuni sensors marked a novel approach to pathogen detection.
空肠弯曲杆菌（Campylobacter jejuni）是世界范围内引起肠胃炎的主要原因，由于目前的检测方法劳力密集且灵敏度不足，因此对其检测提出了重大挑战。开发高效、快速的诊断方法对临床和食品工业应用至关重要；然而，在制造细菌传感器时，生物元素的脆弱性仍然是一个主要障碍。本研究介绍了一种创新的荧光传感器，采用全合成碳点（CDs）功能化分子印迹聚合物（CDs@nanoMIPs）受体来检测这种食源性致病菌。CDs@nanoMIPs的合成采用固相法完成，以空肠梭菌的免疫优势表位作为指导模板。在聚合过程中，氮掺杂CDs被原位合成，并作为荧光标记纳入纳米omip中，约占复合材料的19%。采用透射电子显微镜（TEM）、x射线光电子能谱（XPS）、动态光散射（DLS）、傅里叶变换红外光谱（FT-IR）、荧光显微镜、紫外可见分光光度法和荧光光谱法对合成的纳米材料进行了表征。CDs@nanoMIPs在350 nm激发时表现出显著的蓝色荧光，具有优异的光稳定性和负zeta电位。这些纳米结构的颗粒（59 nm）具有中等的多分散性和球形形貌。FT-IR光谱偏离了传统CDs的光谱，与模板表位更接近。当与C. jejuni接触时，CDs@nanoMIPs诱导荧光强度显著增加，从而实现了高效的细菌检测。该互作对空肠梭菌具有良好的亲和力和敏感性，线性范围为1 × 101-1 × 108 CFU mL - 1 (R2 = 0.98)，检出限为4.6 CFU mL - 1。CDs@nanoMIPs-based空肠c传感器标志着一种新的病原体检测方法。
{"title":"Highly sensitive detection of Campylobacter jejuni using a carbon dot-embedded nanoMIPs fluorescent sensor","authors":"Saliha Dinç , Tim Tjardts , Gregor Maschkowitz , Vivian Lukaszczuk , Seyed Mohammad Taghi Gharibzahedi , Zeynep Altintas","doi":"10.1016/j.microc.2024.112301","DOIUrl":"10.1016/j.microc.2024.112301","url":null,"abstract":"<div><div><em>Campylobacter jejuni</em>, a leading cause of gastroenteritis worldwide, poses significant detection challenges due to the labor-intensive and insufficiently sensitive current methods. Developing efficient, rapid diagnostics is vital for clinical and food industry applications; yet the vulnerability of biological elements in creating bacterial sensors remains a major obstacle. This study introduces an innovative fluorescence-based sensor employing fully synthetic carbon dot (CDs) functionalized molecularly imprinted polymer (CDs@nanoMIPs) receptors to detect this foodborne pathogenic bacterium. The CDs@nanoMIPs synthesis was accomplished using a solid-phase approach, with an immunodominant epitope of <em>C. jejuni</em> serving as a guiding template. During the polymerization process, nitrogen-doped CDs were synthesized in situ and incorporated into nanoMIPs as a fluorescent tag, constituting approximately 19 % of the composite. The synthesized nanomaterials were characterized by employing transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FT-IR), fluorescence microscopy, UV–Vis spectrophotometry, and fluorescence spectrometry. CDs@nanoMIPs demonstrated a notable blue fluorescence when excited at 350 nm, along with excellent photostability and a negative zeta potential. These nanostructured particles (59 nm) exhibited moderate polydispersity and a spherical morphology. The FT-IR spectrum deviated from that of conventional CDs, aligning more closely with the template epitope. When in contact with <em>C. jejuni</em>, CDs@nanoMIPs induced a significant increase in fluorescence intensity, enabling the efficient bacterial detection. This interaction showed exceptional affinity and sensitivity towards <em>C. jejuni</em>, featuring a linear range of 1 × 10<sup>1</sup>–1 × 10<sup>8</sup> CFU mL<sup>−1</sup> (R<sup>2</sup> = 0.98) and a low detection limit of 4.6 CFU mL<sup>−1</sup>. CDs@nanoMIPs-based <em>C. jejuni</em> sensors marked a novel approach to pathogen detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112301"},"PeriodicalIF":4.9,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1016/j.microc.2024.112251
Jing Ma , Yuan Zhang , Yu Bian , Sheng-ye Liu , Xue-song Feng
Hallucinogens, a category of novel psychoactive substances that can be either natural or synthetic, have the potential to induce distortions in the perception of time and space and can result in dissociative thinking, thereby posing a considerable risk to public safety and societal well-being. Consequently, the development of rapid, accurate, and highly sensitive analytical methods for hallucinogens is of paramount importance for safeguarding public health and ensuring social stability. This paper provides a comprehensive review of the current research on the pretreatment and determination methods for hallucinogenic agents in different matrices since 2017. Pretreatment techniques encompass liquid–liquid extraction, liquid phase microextraction, solid phase extraction, and solid phase microextraction, among others. Analytical methodologies include liquid chromatography-based techniques, gas chromatography-based techniques, supercritical fluid chromatography, and sensor-based approaches, among others. This study also examines the advantages and disadvantages, applications, and developmental trends of various pretreatment and detection methods, thereby offering a valuable reference for future research endeavors.
{"title":"Hallucinogens in different complex samples: Recent updates on pretreatment and analysis methods since 2017","authors":"Jing Ma , Yuan Zhang , Yu Bian , Sheng-ye Liu , Xue-song Feng","doi":"10.1016/j.microc.2024.112251","DOIUrl":"10.1016/j.microc.2024.112251","url":null,"abstract":"<div><div>Hallucinogens, a category of novel psychoactive substances that can be either natural or synthetic, have the potential to induce distortions in the perception of time and space and can result in dissociative thinking, thereby posing a considerable risk to public safety and societal well-being. Consequently, the development of rapid, accurate, and highly sensitive analytical methods for hallucinogens is of paramount importance for safeguarding public health and ensuring social stability. This paper provides a comprehensive review of the current research on the pretreatment and determination methods for hallucinogenic agents in different matrices since 2017. Pretreatment techniques encompass liquid–liquid extraction, liquid phase microextraction, solid phase extraction, and solid phase microextraction, among others. Analytical methodologies include liquid chromatography-based techniques, gas chromatography-based techniques, supercritical fluid chromatography, and sensor-based approaches, among others. This study also examines the advantages and disadvantages, applications, and developmental trends of various pretreatment and detection methods, thereby offering a valuable reference for future research endeavors.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"207 ","pages":"Article 112251"},"PeriodicalIF":4.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142720753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1016/j.microc.2024.112263
Kangting Yan , Xiaoyang Lu , Junqi Xiao , Xiaobing Song , Xidan Xu , Jun Guo , Weiguang Yang , Yali Zhang , Yubin Lan
To explore the potential of using flavonoid fluorescence characteristics in citrus leaves and peels for detecting citrus Huanglongbing (HLB), this study focused on Citrus reticulata ‘Shiyue Ju’ and utilized three-dimensional fluorescence spectroscopy for data collection. Excitation-Emission matrices (EEM) of flavonoid standards and citrus leaves and peels were acquired and comparatively analyzed. The concentration of HLB pathogens in citrus tissues was also assessed using qPCR. In order to identify the range of fluorescence bands that are sensitive to HLB, different machine-learning algorithms were used to construct classification models. Support Vector Machine (SVM) was proved most precise, with 93.1 % accuracy for leaves and 86.67 % for peels. The sensitive bands for leaves were EX (Excitation) = 375–455 nm, EM (Emission) = 490–570 nm and for peels were EX = 300–340 nm, EM = 430–470 nm and EX = 330–370 nm, EM = 425–465 nm. These bands correspond closely with the fluorescence signatures of nobiletin, hesperidin, and narirutin. It was further verified that HLB stress affected the changes in the content of flavonoids in the leaves and peels of ‘Shiyue Ju’. This study provides a reference for using flavonoid fluorescence in citrus HLB detection and presents a novel approach for applying spectral imaging techniques in citrus HLB detection.
{"title":"Three-dimensional fluorescence spectral characteristic of flavonoids for citrus Huanglongbing disease early detection","authors":"Kangting Yan , Xiaoyang Lu , Junqi Xiao , Xiaobing Song , Xidan Xu , Jun Guo , Weiguang Yang , Yali Zhang , Yubin Lan","doi":"10.1016/j.microc.2024.112263","DOIUrl":"10.1016/j.microc.2024.112263","url":null,"abstract":"<div><div>To explore the potential of using flavonoid fluorescence characteristics in citrus leaves and peels for detecting citrus Huanglongbing (HLB), this study focused on <em>Citrus reticulata</em> ‘Shiyue Ju’ and utilized three-dimensional fluorescence spectroscopy for data collection. Excitation-Emission matrices (EEM) of flavonoid standards and citrus leaves and peels were acquired and comparatively analyzed. The concentration of HLB pathogens in citrus tissues was also assessed using qPCR. In order to identify the range of fluorescence bands that are sensitive to HLB, different machine-learning algorithms were used to construct classification models. Support Vector Machine (SVM) was proved most precise, with 93.1 % accuracy for leaves and 86.67 % for peels. The sensitive bands for leaves were EX (Excitation) = 375–455 nm, EM (Emission) = 490–570 nm and for peels were EX = 300–340 nm, EM = 430–470 nm and EX = 330–370 nm, EM = 425–465 nm. These bands correspond closely with the fluorescence signatures of nobiletin, hesperidin, and narirutin. It was further verified that HLB stress affected the changes in the content of flavonoids in the leaves and peels of ‘Shiyue Ju’. This study provides a reference for using flavonoid fluorescence in citrus HLB detection and presents a novel approach for applying spectral imaging techniques in citrus HLB detection.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112263"},"PeriodicalIF":4.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-26DOI: 10.1016/j.microc.2024.112213
Şevki Furkan Küçükayar , Şevval Kaya , Veli Şimşek , Mustafa Oguzhan Caglayan , Zafer Üstündağ , Samet Şahin
This study demonstrates the application of carbonised Typha tassel (CTT) in ferrocene-mediated enzymatic glucose biosensing and enzymatic biofuel cell (EnBFC) applications. Typha tassel was carbonised under an inert atmosphere to obtain conductive CTT which was then mixed with an effective electron transfer mediator, ferrocene (Fc) obtaining a redox-active electrode material. The successful immobilisation of the glucose oxidase (GOx) enzyme was performed on a CTT-Fc modified screen-printed electrode followed by a chitosan protective coating. The resulting enzymatic electrode was electrochemically characterised as a glucose biosensor with a working range of 0–10 mM and LOD and LOQ values of 0.19 mM and 0.56 mM, respectively. The developed glucose biosensor also showed good reproducibility and reusability with RSD% values of 6.68 % and 8.75 %, respectively. Furthermore, a real sample demonstration was performed using commercial jam samples with good recovery values. Finally, an EnBFC demonstration was performed using the enzymatic biosensor as an anode and a non-enzymatic cathode prepared using platinum black on gas diffusion carbon electrodes reaching a maximum power density of 3.6 µW cm−2. This study shows the promise of CTT as an alternative to conventional materials in enzymatic biosensor and bioelectronic applications as a suitable, cheap, and sustainable material.
本研究展示了碳化的香菇流苏（CTT）在二茂铁介导的酶促葡萄糖生物传感和酶促生物燃料电池（EnBFC）中的应用。在惰性气氛下碳化，得到导电CTT，然后与有效的电子转移介质二茂铁（Fc）混合，得到氧化还原活性电极材料。葡萄糖氧化酶（GOx）在CTT-Fc修饰的丝网印刷电极上成功固定，然后涂上壳聚糖保护涂层。所得酶促电极经电化学表征为葡萄糖生物传感器，工作范围为0-10 mM， LOD和LOQ值分别为0.19 mM和0.56 mM。该传感器具有良好的重现性和可重复使用性，RSD%分别为6.68%和8.75%。此外，使用具有良好采收率的商业果酱样品进行了实际样品演示。最后，在气体扩散碳电极上使用酶促生物传感器作为阳极和铂黑制备的非酶促阴极进行了EnBFC演示，其最大功率密度为3.6 μ W cm - 2。这项研究表明，CTT作为一种合适、廉价和可持续的材料，有望在酶生物传感器和生物电子应用中替代传统材料。
{"title":"Carbonised Typha tassel-modified enzymatic electrodes for ferrocene-mediated glucose biosensor and glucose/air biofuel cell applications","authors":"Şevki Furkan Küçükayar , Şevval Kaya , Veli Şimşek , Mustafa Oguzhan Caglayan , Zafer Üstündağ , Samet Şahin","doi":"10.1016/j.microc.2024.112213","DOIUrl":"10.1016/j.microc.2024.112213","url":null,"abstract":"<div><div>This study demonstrates the application of carbonised <em>Typha</em> tassel (CTT) in ferrocene-mediated enzymatic glucose biosensing and enzymatic biofuel cell (EnBFC) applications. <em>Typha</em> tassel was carbonised under an inert atmosphere to obtain conductive CTT which was then mixed with an effective electron transfer mediator, ferrocene (Fc) obtaining a redox-active electrode material. The successful immobilisation of the glucose oxidase (GOx) enzyme was performed on a CTT-Fc modified screen-printed electrode followed by a chitosan protective coating. The resulting enzymatic electrode was electrochemically characterised as a glucose biosensor with a working range of 0–10 mM and LOD and LOQ values of 0.19 mM and 0.56 mM, respectively. The developed glucose biosensor also showed good reproducibility and reusability with RSD% values of 6.68 % and 8.75 %, respectively. Furthermore, a real sample demonstration was performed using commercial jam samples with good recovery values. Finally, an EnBFC demonstration was performed using the enzymatic biosensor as an anode and a non-enzymatic cathode prepared using platinum black on gas diffusion carbon electrodes reaching a maximum power density of 3.6 µW cm<sup>−2</sup>. This study shows the promise of CTT as an alternative to conventional materials in enzymatic biosensor and bioelectronic applications as a suitable, cheap, and sustainable material.</div></div>","PeriodicalId":391,"journal":{"name":"Microchemical Journal","volume":"208 ","pages":"Article 112213"},"PeriodicalIF":4.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142743673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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