Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2024-08-27 DOI:10.1584/jpestics.d24-005
Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani
{"title":"Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves","authors":"Munekazu Ogawa, Akihiro Nishimura, Satoshi Araki, Yuzuka Abe, Nanami Kuwahara, Yohei Fukumori, Kazumi Suzuki, Shigeru Mitani","doi":"10.1584/jpestics.d24-005","DOIUrl":null,"url":null,"abstract":"</p><p>Pyriofenone demonstrates outstanding efficacy in controlling powdery mildew. We investigated the impact of pyriofenone on the infection processes and cytological features of <i>Blumeria graminis</i> f. sp. <i>tritici</i> on wheat leaves. The preventive application of pyriofenone before inoculation did not inhibit conidial germination but effectively suppressed both appressorial and haustorial formation. Notably, haustorial formation was effectively inhibited, resulting in the complete suppression of successive lesion development and sporulation. Curative application of pyriofenone after inoculation also inhibited lesion expansion and sporulation. Furthermore, it had considerable impact on the morphogenesis of powdery mildew fungus. We observed multi-formed secondary appressoria, shrunken or bifurcated hyphae, abnormal conidiophores, and clubbed conidia-like structures. Subsequently, we employed a histochemical approach to analyze the localization of essential components for the polar growth of fungal hyphae. Pyriofenone induced mislocalization of the actin cytoskeleton, β-glucan and cytoplasmic vesicles, although it did not affect tubulin orientation.</p>\n<p></p>\n<img alt=\"\" src=\"https://www.jstage.jst.go.jp/pub/jpestics/advpub/0/advpub_D24-005/figure/advpub_D24-005.png\"/>\n<span style=\"padding-left:5px;\">Fullsize Image</span>","PeriodicalId":16712,"journal":{"name":"Journal of Pesticide Science","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Pesticide Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1584/jpestics.d24-005","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Pyriofenone demonstrates outstanding efficacy in controlling powdery mildew. We investigated the impact of pyriofenone on the infection processes and cytological features of Blumeria graminis f. sp. tritici on wheat leaves. The preventive application of pyriofenone before inoculation did not inhibit conidial germination but effectively suppressed both appressorial and haustorial formation. Notably, haustorial formation was effectively inhibited, resulting in the complete suppression of successive lesion development and sporulation. Curative application of pyriofenone after inoculation also inhibited lesion expansion and sporulation. Furthermore, it had considerable impact on the morphogenesis of powdery mildew fungus. We observed multi-formed secondary appressoria, shrunken or bifurcated hyphae, abnormal conidiophores, and clubbed conidia-like structures. Subsequently, we employed a histochemical approach to analyze the localization of essential components for the polar growth of fungal hyphae. Pyriofenone induced mislocalization of the actin cytoskeleton, β-glucan and cytoplasmic vesicles, although it did not affect tubulin orientation.

Abstract Image Fullsize Image
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
吡蚜酮对小麦叶片上禾谷蓝霉菌感染过程和细胞学特征的影响
吡唑醚菌酯在防治白粉病方面表现出卓越的功效。我们研究了吡唑醚菌酯对三尖杉白粉病在小麦叶片上的侵染过程和细胞学特征的影响。在接种前预防性施用吡唑醚菌酯并不能抑制分生孢子的萌发,但能有效抑制附着孢子和簇孢子的形成。值得注意的是,寄主形成被有效抑制,从而完全抑制了连续病变的发展和孢子的产生。在接种后施用吡唑醚菌酯也能抑制病斑的扩展和孢子的产生。此外,它对白粉病菌的形态发生也有相当大的影响。我们观察到了多形成的次生附属物、萎缩或分叉的菌丝、异常的分生孢子梗和棍棒状的分生孢子结构。随后,我们采用组织化学方法分析了真菌菌丝极性生长的重要成分的定位。Pyriofenone 诱导了肌动蛋白细胞骨架、β-葡聚糖和细胞质囊泡的错误定位,但并不影响微管蛋白的定向。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
期刊最新文献
Effect of pyriofenone on the infection processes and cytological features of Blumeria graminis on wheat leaves Synthesis and biological evaluation of burnettiene A derivatives enabling discovery of novel fungicide candidates. Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana A reliable quantification of organophosphorus pesticides in brown rice samples for proficiency testing using Japanese official analytical method, QuEChERS, and modified QuEChERS combined with isotope dilution mass spectrometry Bacterial Pesticides: Mechanism of Action, Possibility of Food Contamination, and Residue Analysis Using MS
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1