In-house production of [99mTc][Tc-HYNIC-TATE] cold kit for the diagnosis of neuroendocrine tumors in Pakistan: pre-clinical and clinical evaluation of indigenously manufactured single and dual vial kit formulation

IF 1.5 3区 化学 Q3 CHEMISTRY, ANALYTICAL Journal of Radioanalytical and Nuclear Chemistry Pub Date : 2024-09-09 DOI:10.1007/s10967-024-09726-w
Shakera Khatoon Rizvi, Saima Tariq, Farkhanda Gilani, Amna Javed, Iqra Rafique
{"title":"In-house production of [99mTc][Tc-HYNIC-TATE] cold kit for the diagnosis of neuroendocrine tumors in Pakistan: pre-clinical and clinical evaluation of indigenously manufactured single and dual vial kit formulation","authors":"Shakera Khatoon Rizvi, Saima Tariq, Farkhanda Gilani, Amna Javed, Iqra Rafique","doi":"10.1007/s10967-024-09726-w","DOIUrl":null,"url":null,"abstract":"<p>Neuroendocrine tumors (NETs) as compared to its octreotide (NOC) counterpart when labeled with In-111 and Tc-99m. This study was aimed to develop easy to use, cost effective, and commercially available diagnostic kit of [<sup>99m</sup>Tc][Tc-HYNIC-TATE] (Tyr3-Octreotate) indigenously to diagnose neuroendocrine tumors in Pakistan. HYNIC-TATE cold kit was optimized at 20µg of peptide (Octreotate), following co-ligand exchange labeling approach using EDDA/TRICINE (3:6 mg) while heating at 90 °C for 20 min. Radiochemical purity of [<sup>99m</sup>Tc][Tc-HYNIC-TATE] was &gt; 98 ± 0.14% when labeled with 550–850 MBq of Tc-99m and stable upto 12 h. Preclinical &amp; clinical investigations validated the higher sensitivity and specificity of the developed kits for spot-on detection of neuroendocrine tumours (NETs).</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":661,"journal":{"name":"Journal of Radioanalytical and Nuclear Chemistry","volume":null,"pages":null},"PeriodicalIF":1.5000,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Radioanalytical and Nuclear Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10967-024-09726-w","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Neuroendocrine tumors (NETs) as compared to its octreotide (NOC) counterpart when labeled with In-111 and Tc-99m. This study was aimed to develop easy to use, cost effective, and commercially available diagnostic kit of [99mTc][Tc-HYNIC-TATE] (Tyr3-Octreotate) indigenously to diagnose neuroendocrine tumors in Pakistan. HYNIC-TATE cold kit was optimized at 20µg of peptide (Octreotate), following co-ligand exchange labeling approach using EDDA/TRICINE (3:6 mg) while heating at 90 °C for 20 min. Radiochemical purity of [99mTc][Tc-HYNIC-TATE] was > 98 ± 0.14% when labeled with 550–850 MBq of Tc-99m and stable upto 12 h. Preclinical & clinical investigations validated the higher sensitivity and specificity of the developed kits for spot-on detection of neuroendocrine tumours (NETs).

Graphical Abstract

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在巴基斯坦自行生产用于诊断神经内分泌肿瘤的[99m锝][Tc-HYNIC-TATE]冷试剂盒:对本国生产的单瓶和双瓶试剂盒配方进行临床前和临床评估
与用 In-111 和 Tc-99m 标记的辛孕肽 (NOC) 相比,神经内分泌肿瘤 (NET)。本研究的目的是在巴基斯坦本土开发易于使用、具有成本效益和商业化的[99mTc][Tc-HYNIC-TATE](Tyr3-Octreotate)诊断试剂盒,用于诊断神经内分泌肿瘤。HYNIC-TATE冷试剂盒采用EDDA/TRICINE(3:6 mg)共配体交换标记法,在90°C下加热20分钟,肽(Octreotate)含量优化为20µg。临床前和临床研究验证了所开发试剂盒在定点检测神经内分泌肿瘤(NET)方面具有更高的灵敏度和特异性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
2.80
自引率
18.80%
发文量
504
审稿时长
2.2 months
期刊介绍: An international periodical publishing original papers, letters, review papers and short communications on nuclear chemistry. The subjects covered include: Nuclear chemistry, Radiochemistry, Radiation chemistry, Radiobiological chemistry, Environmental radiochemistry, Production and control of radioisotopes and labelled compounds, Nuclear power plant chemistry, Nuclear fuel chemistry, Radioanalytical chemistry, Radiation detection and measurement, Nuclear instrumentation and automation, etc.
期刊最新文献
Uranium isotopes and several heavy elements in selected waters in Quang Nam-Da Nang provinces, Central Vietnam Instrumental neutron activation analysis of Ghanaian hot pepper (Capsicum spp. l.) collections 89Zr PET imaging guided validation of the medicinal potentiality of UiO-66 based nano drug delivery system Correlation and statistical analysis between natural radioactivity and hazards in rocks from Kolar taluk, Karnataka Feasibility of laser induced breakdown spectroscopy for quantification of zirconium in nuclear streams
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1