Development of a new test design to investigate the degradation of pesticides in soil under sunlight conditions

Abstract

Pesticides applied to soil surface are subject to photodegradation if the parent molecule is sensitive to UV-light absorption. Photodegradation studies are therefore mandatory for the registration of plant protection products to provide data on the degradation rate and on the nature of photoproducts formed. In general, sunlight is simulated in these studies with xenon lamps, e.g., a Suntest® device. Surface application on very thin soil layers followed by direct irradiation is common practice, but the control of the boundary conditions, i.e. soil temperature and moisture, to maintain the structure and viability of the soil is challenging. A homogeneous and stable soil microclimate is crucial to compare the degradation data of the test item from the irradiated soil samples to the dark controls as well as to the results from the aerobic soil metabolism study. After trying different scale-up test systems with the UV-sensitive herbicide imazamox as comparative test item, a new soil photolysis test system was developed which is manageable in the laboratory and enables a more favorable management of the boundary conditions, especially with regard to the soil moisture and temperature. For this, the solar simulator SolarConstant® 1200, equipped with metal halide lamps Radium HRI-TS 1000W/D/S/PRO, was installed by Atlas Ametek (Germany) in a temperature controllable walk-in incubation chamber with aluminum racks and reflectors to minimize diffuse light and to maintain a homogenous temperature of 22(± 1)°C within the irradiated soil. Borosilicate glass vessels with an inner diameter of 10 cm and a maximum height of 9 cm, covered by quartz glass, were used for the incubation of the applied soil under light. Contrary to the imazamox degradation half-lives obtained with the Suntest® test system, where an unusual slower degradation was observed under light compared to the dark controls, the results from the new SolarConstant® study design showed the expected faster degradation under light. Hence, it can be concluded that the experimental boundary conditions of the new test system are more suitable to maintain the viablity of the irradiated soil. Since no adjustments of the soil water content were needed, compared to daily water adjustments for thin soil layers incubated under a Suntest®, drying–wetting cycles are eliminated and microbial-induced soil processes are maintained.