Synthesis of water-soluble CuInS2@ZnS quantum dots using amino acids as ligands and their application in smartphone-based visual detection of ciprofloxacin

Abstract

Ternary I-III-VI quantum dots, unlike traditional binary counterparts, offer advantages like low toxicity, cadmium-free composition, tunable electronic properties, and near-infrared light emission, making them increasingly popular for their cost-effectiveness and versatile applications in light emission. In this study, monodispersive, water-soluble, highly emissive CuInS₂@ZnS quantum dots were synthesized using cysteine and threonine as ligands by a simple one-pot solution method. By adjusting, the ratio of cysteine to threonine to 1:5 and the heating time during the synthesis process to 40 min, CuInS₂@ZnS quantum dots achieved the highest fluorescence intensity. Then, CuInS₂@ZnS quantum dots were applied for ciprofloxacin (CIP) detection in food sample, showing a linear range of 2–18 μg/ml and a lowest detection limit of 0.28 μg/ml. The fluorescent probe was successfully established by quenching process as CIP concentration increasing, due to photoinduced electron transfer. Experimental results demonstrated the detection method has high sensitivity and good selectivity. We, then utilized smartphones to visually distinguish CIP concentrations based on the colors emitted by the CuInS₂@ZnS quantum dots under UV light. The method was finally effectively to detect residual CIP in porcine kidneys, with recoveries of 92%–98%. This approach has promising potential for enhancing food safety testing.