Rapid fabrication of modular 3D paper-based microfluidic chips using projection-based 3D printing

IF 8.1 1区医学 Q1 ENGINEERING, BIOMEDICAL Bio-Design and Manufacturing Pub Date : 2024-08-17 DOI:10.1007/s42242-024-00298-y

Mingjun Xie, Zexin Fu, Chunfei Lu, Sufan Wu, Lei Pan, Yong He, Yi Sun, Ji Wang

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Abstract

Paper-based microchips have different advantages, such as better biocompatibility, simple production, and easy handling, making them promising candidates for clinical diagnosis and other fields. This study describes a method developed to fabricate modular three-dimensional (3D) paper-based microfluidic chips based on projection-based 3D printing (PBP) technology. A series of two-dimensional (2D) paper-based microfluidic modules was designed and fabricated. After evaluating the effect of exposure time on the accuracy of the flow channel, the resolution of this channel was experimentally analyzed. Furthermore, several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods, with good channel connectivity. Scaffold-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be feasible. Furthermore, by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips, multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paper-based microfluidic chips, confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications.

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利用基于投影的三维打印技术快速制造模块化三维纸基微流体芯片

纸基微芯片具有生物相容性好、制作简单、易于处理等优点，有望应用于临床诊断等领域。本研究介绍了一种基于投影三维打印（PBP）技术的模块化三维（3D）纸基微流控芯片制造方法。研究人员设计并制作了一系列二维（2D）纸基微流体模块。在评估了曝光时间对流道精度的影响后，对该流道的分辨率进行了实验分析。此外，在二维纸基微流体芯片的基础上，采用不同方法组装了多个三维纸基微流体芯片，并实现了良好的通道连接。基于三维纸基微流体芯片的基于支架的二维和基于水凝胶的三维细胞培养系统被证实是可行的。此外，通过将挤压式三维生物打印技术与所提出的三维纸基微流控芯片相结合，在三维纸基微流控芯片上直接打印三维水凝胶结构，建立了多器官微流控芯片，证实了所制备的模块化三维纸基微流控芯片可应用于各种生物医学领域。

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来源期刊

Bio-Design and Manufacturing Materials Science-Materials Science (miscellaneous)

CiteScore

13.30

自引率

7.60%

发文量

148

期刊介绍： Bio-Design and Manufacturing reports new research, new technology and new applications in the field of biomanufacturing, especially 3D bioprinting. Topics of Bio-Design and Manufacturing cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.