A fast, sensitive, greener, and stability-indicating HPLC method for the standardization and quantitative determination of chlorhexidine acetate in commercial products

Abstract

The goal of the proposed work was to create and verify a fast, sensitive, greener, and stability-indicating high-performance liquid chromatography (HPLC) method to quantify chlorhexidine acetate (CHDA) in commercial products. The developed method was validated for numerous validation metrics and greenness. The greener mobile phase was made up of a ternary mixture of ethanol, water, and glacial acetic acid (50:49:1 v/v/v). CHDA was detected at a wavelength of 265 nm. The developed HPLC method showed a coefficient of determination of 0.9981 and was linear in the 1–100 µg/mL range. In addition, the developed method for CHDA analysis was rapid, accurate, precise, robust, and sensitive. The outstanding greenness profile was indicated by the derived values of the Analytical Eco-Scale, ChlorTox, and AGREE scales for the current approach, which are 89, 0.74 g, and 0.77, respectively. With its breakdown products present, the proposed analytical approach was still able to identify CHDA, demonstrating its selectivity- and stability-indicating qualities. Two distinct commercial products, A and B, were found to contain 1.96 and 2.05% w/v of CHDA, respectively. These results revealed that CHDA in commercially accessible products can be routinely standardized and quantified using the proposed HPLC approach.