CircBIRC6 affects prostate cancer progression by regulating miR-574-5p and DNAJB1.

IF 4.6 4区医学 Q2 ONCOLOGY Cancer Biology & Therapy Pub Date : 2024-09-11 DOI:10.1080/15384047.2024.2399363

Bin Zhao,Jinye Yang,Fengming Ran,Yuanlong Shi,Libo Yang,Yuanpeng Duan,Zhiyu Shi,Xin Li,Jianpeng Zhang,Zhiyao Li,Jiansong Wang

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Abstract

BACKGROUND Prostate cancer (PCa) is among the three main types of cancer. Although prostate-specific antigen (PSA) is routinely tested, it has disadvantages, such as poor prognostic ability. Therefore, finding more PCa markers and therapeutic targets remains a subject of study. CircRNAs have been found to have regulatory roles in various diseases, such as diabetes, Central Nervous System (CNS) neuropathy, etc. where their application in cancer is even more valuable. Therefore, this paper aims to search for differentially expressed circRNAs in PCa and find downstream targeting pathways related to autophagy. METHOD By detecting the expression of circRNA in the samples, hsa_circ_0119816 was finally identified as the research target. The properties of circRNA were verified by RNase R, actinomycin D, and fluorescence in situ hybridization (FISH). The downstream target miRNAs and target proteins were predicted by an online database, and the targeting relationship was verified using dual luciferase and RNA Immunoprecipitation. The effects of circRNAs and their downstream signalling pathways on prostate cancer cell proliferation, migration, EMT and autophagy were examined by CCK-8, Transwell, immunofluorescence and Western blotting. RESULTS CircBIRC6 is highly expressed in prostate cancer samples. Knockdown of its expression inhibits cell proliferation, invasion, EMT and autophagy and promotes apoptosis. CircBIRC6/miRNA-574-5p/DNAJB1 is a molecular axis that regulates prostate cancer cells.

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CircBIRC6 通过调控 miR-574-5p 和 DNAJB1 影响前列腺癌的进展。

背景前列腺癌（PCa）是三大癌症之一。虽然前列腺特异性抗原（PSA）是常规检测指标，但它也有缺点，如预后能力差。因此，寻找更多的 PCa 标志物和治疗靶点仍是一个研究课题。目前已发现 CircRNAs 在糖尿病、中枢神经系统（CNS）神经病变等多种疾病中具有调控作用，其在癌症中的应用价值更高。因此，本文旨在寻找 PCa 中差异表达的 circRNA，并找到与自噬相关的下游靶向通路。方法通过检测样本中 circRNA 的表达，最终确定 hsa_circ_0119816 为研究靶点。通过 RNase R、放线菌素 D 和荧光原位杂交（FISH）验证了 circRNA 的特性。通过在线数据库预测了下游靶miRNA和靶蛋白，并利用双荧光素酶和RNA免疫沉淀验证了靶向关系。通过CCK-8、Transwell、免疫荧光和Western印迹检测了circRNAs及其下游信号通路对前列腺癌细胞增殖、迁移、EMT和自噬的影响。结果CircBIRC6在前列腺癌样本中高表达，抑制其表达可抑制细胞增殖、侵袭、EMT和自噬，并促进细胞凋亡。CircBIRC6/miRNA-574-5p/DNAJB1是调控前列腺癌细胞的分子轴。

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来源期刊

Cancer Biology & Therapy 医学-肿瘤学

CiteScore

7.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Cancer, the second leading cause of death, is a heterogenous group of over 100 diseases. Cancer is characterized by disordered and deregulated cellular and stromal proliferation accompanied by reduced cell death with the ability to survive under stresses of nutrient and growth factor deprivation, hypoxia, and loss of cell-to-cell contacts. At the molecular level, cancer is a genetic disease that develops due to the accumulation of mutations over time in somatic cells. The phenotype includes genomic instability and chromosomal aneuploidy that allows for acceleration of genetic change. Malignant transformation and tumor progression of any cell requires immortalization, loss of checkpoint control, deregulation of growth, and survival. A tremendous amount has been learned about the numerous cellular and molecular genetic changes and the host-tumor interactions that accompany tumor development and progression. It is the goal of the field of Molecular Oncology to use this knowledge to understand cancer pathogenesis and drug action, as well as to develop more effective diagnostic and therapeutic strategies for cancer. This includes preventative strategies as well as approaches to treat metastases. With the availability of the human genome sequence and genomic and proteomic approaches, a wealth of tools and resources are generating even more information. The challenge will be to make biological sense out of the information, to develop appropriate models and hypotheses and to translate information for the clinicians and the benefit of their patients. Cancer Biology & Therapy aims to publish original research on the molecular basis of cancer, including articles with translational relevance to diagnosis or therapy. We will include timely reviews covering the broad scope of the journal. The journal will also publish op-ed pieces and meeting reports of interest. The goal is to foster communication and rapid exchange of information through timely publication of important results using traditional as well as electronic formats. The journal and the outstanding Editorial Board will strive to maintain the highest standards for excellence in all activities to generate a valuable resource.