Integrated transcriptomic and miRNA-seq analysis of Pak-choi provides insight into the molecular mechanisms involved in the preservation of postharvest quality by white LED light irradiation

IF 6.4 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2024-09-13 DOI:10.1016/j.postharvbio.2024.113183

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Abstract

Low-intensity white light-emitting diode (LED) irradiation effectively delays postharvest senescence in pak-choi at 20 °C and 90 % relative humidity. In this study, RNA-seq and miRNA-seq analyses were conducted on samples collected at 0 d (initial sample) and 5 d (CK and LED-treated samples) to evaluate the regulatory effects of white LED irradiation on miRNA. The resulting data revealed a total of 23 differentially expressed miRNAs (DEmiRNAs) in the CK/LED group on 5 d of storage. LED irradiation induced the expression of AP2 family genes and the biosynthesis of aliphatic glucosinolates by modulating the level of bra-miR172 family miRNAs. LED irradiation also stimulated the expression of genes associated with the photosynthetic pathway in pak-choi and regulated biosynthesis of ascorbic acid and resistance-associated transcription factors by modulating the level of novel miRNAs. Furthermore, LED irradiation suppressed cellulase and polygalacturonase activity, and maintained the pectin content. These findings demonstrate the ability of white LED light irradiation to significantly delay the postharvest senescence process in pak-choi by modulating miRNA expression.

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对白菜的转录组和 miRNA-seq 综合分析有助于深入了解白 LED 光照射保持采后品质的分子机制

在 20 °C、相对湿度为 90 % 的条件下，低强度白色发光二极管（LED）辐照可有效延缓椿采后衰老。本研究对 0 d（初始样品）和 5 d（CK 和 LED 处理样品）采集的样品进行了 RNA-seq 和 miRNA-seq 分析，以评估白光 LED 照射对 miRNA 的调控作用。结果显示，CK/LED组在储存5天后共有23个差异表达的miRNA（DEmiRNA）。LED照射通过调节bra-miR172家族miRNA的水平，诱导了AP2家族基因的表达和脂肪族葡萄糖苷酸盐的生物合成。LED 照射还刺激了椿树光合作用途径相关基因的表达，并通过调节新型 miRNA 的水平来调节抗坏血酸和抗性相关转录因子的生物合成。此外，LED 照射还抑制了纤维素酶和聚半乳糖醛酸酶的活性，并保持了果胶含量。这些研究结果表明，白光 LED 能够通过调节 miRNA 的表达，显著延缓椿采后的衰老过程。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.