Postharvest Biology and Technology最新文献

{"title":"Development of a novel packaging box with on-demand ripening function for fast kiwifruit logistics","authors":"Ying Liu ,&nbsp;Jie Hao ,&nbsp;Peiji Yang ,&nbsp;Zhiguo Li ,&nbsp;Fideline Tchuenbou-Magaia ,&nbsp;Wenzhi Tang","doi":"10.1016/j.postharvbio.2025.113595","DOIUrl":"10.1016/j.postharvbio.2025.113595","url":null,"abstract":"<div><div>The acceptance of kiwifruit by consumers is significantly affected by its prolonged post-ripening period and slow ripening. In this study, a novel type of kiwifruit packing box, containing ripening function was developed for fast logistics. The box utilizes an integrated circuit board to precisely control the ultrasonic atomization release of the ripening agent. The partition board divides the packaging box into two ripening allowing on-demand fruit ripening in different areas of packaging box based on the consumer's preference. The optimal ripening method, concentration, and dosage of a model ripening agent, ethephon, were determined. Results showed that spraying 12.5 mL of 1000 mg/kg ethephon solution achieved the best ripening effect. ‘<em>Cuixiang</em>’ kiwifruit required only 2 days to reach ready-to-eat status, 6 days earlier than the untreated group, whereas ‘<em>Xuxiang</em>’ kiwifruit required only 3 days, 12 days earlier than the untreated group. Faster ripening was achieved with a one-time application of ethephon when compared with other method including multiple application treatment, the solid ethylene treatment, traditional mixed fruit packaging with apple and banana-induced ripening and untreated fruit. This revolutionary kiwifruit e-packaging with ripening function provides consumers with a self-controlled ripening device and solution after purchasing kiwifruit, effectively meeting the demand for ready-to-eat kiwifruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113595"},"PeriodicalIF":6.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ethylene-induced MdDof1.2 activates MdAMY1 to promote starch degradation in post-harvest apple fruit","authors":"Yingcong Cai ,&nbsp;Guangxin Yang ,&nbsp;Yingda Wang ,&nbsp;Le Sun ,&nbsp;Bowen Sun ,&nbsp;Xihong Ma ,&nbsp;Aide Wang ,&nbsp;Tong Li","doi":"10.1016/j.postharvbio.2025.113564","DOIUrl":"10.1016/j.postharvbio.2025.113564","url":null,"abstract":"<div><div>Starch degradation is a critical physiological change that occurs during the postharvest storage of apples, significantly affecting fruit quality. Ethylene, a key hormone in climacteric fruits, is known to promote this process. However, the underlying mechanism remains poorly understood. This study aimed to elucidate how ethylene mediates starch degradation in apples by focusing on its role in regulating specific gene expression. Using ethylene treatment and gene expression analysis, we identified two key genes, <em>MdAMY1</em> and <em>MdBAM8</em>, that are upregulated during apple storage. In addition, we discovered a DOF-type transcription factor, MdDof1.2, whose expression is induced by ethylene. Functional assays demonstrated that MdDof1.2 directly binds to the promoter of <em>MdAMY1,</em> enhancing its transcription. Overexpression of <em>MdDof1.2</em> in apple fruits significantly increased <em>MdAMY1</em> expression and accelerated starch degradation during storage. These findings reveal a novel gene regulatory network in which ethylene regulates starch metabolism through transcriptional activation of key genes. This study provides new insights into the molecular mechanisms underlying postharvest fruit metabolism, with potential applications for improving storage strategies and extending fruit shelf life.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113564"},"PeriodicalIF":6.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteasome shuttle protein DSK2b regulates the enzymatic browning of potato","authors":"Liqun Gong ,&nbsp;Longhao Liu ,&nbsp;Yuanning Zhang ,&nbsp;Simon Stael ,&nbsp;Huiqing Liu ,&nbsp;Qingguo Wang ,&nbsp;Tiantian Dong","doi":"10.1016/j.postharvbio.2025.113566","DOIUrl":"10.1016/j.postharvbio.2025.113566","url":null,"abstract":"<div><div>Potatoes are susceptible to enzymatic browning, which affects the processing and visual quality of potato produce. The proteasome shuttle protein DSK2b was screened to be interacted with two browning-resistant protease inhibitors and <em>DSK2b</em> exhibited higher expression in browning-resistant potato cultivar. Further experiments found that <em>DSK2b</em> expression increased with browning, suggesting a potential browning-regulatory role. Therefore, the effect of <em>DSK2b</em> on potato browning and related mechanisms was investigated by altering <em>DSK2b</em> expression levels. Results showed that <em>DSK2b</em>-overexpression alleviated the browning of potato pulps while RNA-interference of <em>DSK2b</em> aggravated the phenotype. <em>DSK2b</em> negatively affected the free amino acid accumulation, which contributed to potato browning. Besides, <em>DSK2b</em> negatively regulated phenylpropanoid metabolism by modulating key enzymes of PAL, 4CL, and C4H. Concurrently, <em>DSK2b</em> modulated the gene expression and activities of PPO and POD. Transcriptomic and proteomic analyses revealed that <em>DSK2b</em> regulated potato browning, which was closely related to protease inhibitors. This study suggests that <em>DSK2b</em> regulates enzymatic browning in potatoes, offering a novel strategy for mitigating enzymatic browning.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113566"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in cell wall characteristics and expression of MiERF12, MiERF109-like, and MiERF113 during mango softening","authors":"Yuhan Long ,&nbsp;Lihong Xie ,&nbsp;Ping Yi ,&nbsp;Fang Huang ,&nbsp;Min Huang ,&nbsp;Ting Gan ,&nbsp;Jian Sun ,&nbsp;Li Li ,&nbsp;Hao Dong ,&nbsp;Qi Wei ,&nbsp;Zhenmei Fan ,&nbsp;Qiuyu Cheng","doi":"10.1016/j.postharvbio.2025.113532","DOIUrl":"10.1016/j.postharvbio.2025.113532","url":null,"abstract":"<div><div>Mango fruit softening is the primary reason for the reduction in shelf life and the subsequent decrease in market value. In this study, we investigated the roles of ethylene response factor (ERF) in regulating mango fruit softening in response to 1-methylcyclopropene (1-MCP) and ethylene (ETH) treatments was investigated. The action mechanism of the cellular microstructure, key structural components of the cell wall, cell wall metabolizing enzymes, and the ERF were evaluated. Results showed that mango softening was induced by ETH and inhibited by 1-MCP. ETH treatment caused cellular microstructural changes, including cell wall thinning and distortion, while 1-MCP treatment preserved cell wall integrity. Biochemical and molecular assays indicated that 1-MCP and ETH regulated fruit softening by altering cell wall polysaccharide fractions, cell wall degrading enzyme activities (pectate lyase, pectin methylesterase, β-galactosidase, cellulase) and their gene expression (<em>MiPLY8</em>, <em>MiPME3</em>, <em>Miβ-CAL</em>, <em>MiPG2</em>). Meanwhile, ETH strongly induced the expression of <em>MiERF12</em>, <em>MiERF021-like</em>, <em>MiERF109-like</em>, <em>and MiERF113.</em> Virus-induced gene silencing (VIGS) results indicated <em>MiERF109-like</em> and <em>MiERF113</em> promoted mango fruit softening, whereas <em>MiERF12</em> inhibited softening. These findings emphasize that <em>MiERF12</em>, <em>MiERF109-like</em> and <em>MiERF113</em> could play an important role in regulating postharvest ripening and softening of mango, and clarify the potential association of ERFs with cell wall metabolism and structure in mango.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113532"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The function of BoVDAC3 from broccoli in oxidative stress response and programmed cell death in BY-2 cells","authors":"Yuan Qian ,&nbsp;Jiahui Chen ,&nbsp;Shifeng Cao ,&nbsp;Min Luo ,&nbsp;Wenhui Jiao ,&nbsp;Yi Chen ,&nbsp;Yingying Wei ,&nbsp;Xingfeng Shao ,&nbsp;Feng Xu","doi":"10.1016/j.postharvbio.2025.113582","DOIUrl":"10.1016/j.postharvbio.2025.113582","url":null,"abstract":"<div><div>Voltage dependent anion channel protein (VDAC) had been shown to played a critical role in programmed cell death (PCD). However, the functional role of <em>BoVDAC3</em> in oxidative stress response and PCD process of postharvest broccoli remains unclear. In this study, <em>BoVDAC3</em> was overexpressed in tobacco bright yellow-2 (BY-2) cells to investigate its function. The results showed that the cell morphology and nuclear integrity of transgenic BY-2 cells were severely disrupted after oxidative stress compared with wild-type (WT) cells. <em>BoVDAC3</em> overexpressing dreadfully increased the content of reactive oxygen species (ROS) and malondialdehyde (MDA) in BY-2 cells, while simultaneously decreasing proline (Pro) synthesis and impairing the function of the AsA-GSH cycle, along with key antioxidant enzymes. Moreover, the expression levels of AsA-GSH cycle-related genes (<em>NtGST</em>, <em>NtAO</em>, <em>NtAPX</em>), Pro synthesis-related genes (<em>NtP5CR</em>, <em>NtP5CS</em>, <em>Ntδ-OAT</em>), antioxidant enzyme genes (<em>NtSOD</em>, <em>NtPOD</em>, <em>NtCAT</em>), and the anti-apoptotic gene (<em>NtDAD1</em>) were markedly reduced in the transgenic cells compared to the control group following H₂O₂ treatment. Conversely, the expression levels of apoptotic genes (<em>NtSIPK</em>, <em>NtERF3</em>) were considerably elevated under the same experimental conditions. Furthermore, treatment with 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS), a VDAC inhibitor, produced the opposite phenotype to <em>BoVDAC3</em>-overexpressing cells. These findings suggest that <em>BoVDAC3</em> overexpression may accelerate ROS accumulation, thereby inducing PCD in BY-2 cells.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113582"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhance the quality of “Tongling white ginger” by blue LED treatment: Based on delaying oxidative metabolism and cell wall metabolism pathways","authors":"Jiayi Wang,&nbsp;Xin Wang,&nbsp;Xiaomin Yang,&nbsp;Fang Zhang","doi":"10.1016/j.postharvbio.2025.113568","DOIUrl":"10.1016/j.postharvbio.2025.113568","url":null,"abstract":"<div><div>Because of the high water content and thin peel of ‘Tongling white ginger’, softening is the most important factor limiting its postharvest storage and transportation. In this study, blue LED treatment effectively delayed the softening of ‘Tongling white ginger’ after harvesting by regulating oxidative metabolism and cell wall metabolism, thereby maintaining its quality. The results showed that blue LED treatment delayed the decline in firmness of ‘Tongling white ginger’, maintained higher storage quality, and effectively extended its storage period. Compared with the dark group, blue LED treatment significantly reduced the water-soluble pectin content while increased the protopectin, hemicellulose and cellulose contents of ‘Tongling white ginger’. This was mainly due to the inhibition of cell wall-degrading enzyme activities and the reduction of transcription levels of genes related to cell wall metabolism. Additionally, blue LED activated the antioxidant system of ‘Tongling white ginger’, contributing to delaying its postharvest senescence.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113568"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring eco-friendly strategies for the management of blue mold of apple caused by Penicillium crustosum thom","authors":"Santosh Watpade ,&nbsp;Isha Devi ,&nbsp;Samadhan Yuvraj Bagul ,&nbsp;G.N. Khadke ,&nbsp;Dinesh Kumar ,&nbsp;Hema Kumari ,&nbsp;Rishav Kumar ,&nbsp;Jitender Kumar ,&nbsp;Kallol Kumar Pramanick ,&nbsp;Dharam Pal ,&nbsp;Priyank H. Mhatre ,&nbsp;Santosh C. Kedar","doi":"10.1016/j.postharvbio.2025.113581","DOIUrl":"10.1016/j.postharvbio.2025.113581","url":null,"abstract":"<div><div>Blue mold disease, incited by <em>Penicillium</em> sp is an important post-harvest disease of apple adversely affecting their quality. The present study showed about ten percent of the fruit were affected by this disease. The causative organism of blue mold was isolated and identified as <em>Penicillium crustosum</em> Thom using morphological characteristics, molecular analysis (including <em>Beta-tubulin</em>, <em>LSU</em>, and <em>ITS</em> gene sequences), and pathogenicity tests. Following identification, green strategies such as essential oils (Eos, 8 nos) and biocontrol agents (BCAs, 6 nos) were evaluated to manage the disease. Initially, these green chemicals were assessed using the poisoned food technique. Among the eight EOs tested, <em>Ocimum tenuiflorum</em> L. and <em>O. sanctum</em> L. were selected for further studies as they showed &gt; 90 percent inhibition of mycelial growth at all tested concentrations. In the sandwich method assay, <em>O. sanctum</em> demonstrated 95 % inhibition at a 0.5 % concentration and achieved 100 % inhibition at 1.0 % concentration, outperforming <em>O. tenuiflorum</em> which showed 93.3 % and 95.0 % inhibition at 0.5 % and 1.0 % concentration, respectively. Among BCAs (<em>Trichoderma</em> spp.) the inhibition of <em>P. crustosum</em> growth varied significantly, <em>T. longibrachiatum</em> (isolate 2) showed the highest mycelial growth inhibition (83.15 %) therefore selected for further studies. The most effective treatments, identified through <em>in vitro</em> experiments, when tested on apple fruit, the use of spray treatment of 0.5 % <em>O. tenuiflorum</em> EO or fumigation treatments of 1 % <em>O. sanctum</em> EO, gave more protection therefore recommended for management of blue mold of apple. Based on available information, this appears to be the first documented case of apple blue mold caused by <em>P. crustosum</em> in India, along with attempts at its ecofriendly management.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113581"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the biocontrol performance of Bacillus velezensis against postharvest diseases of eggplants and the underlying action mechanisms in soft rot management","authors":"Xiaoyun Zhang ,&nbsp;Junyi Wang ,&nbsp;Yu Xin ,&nbsp;Esa Abiso Godana ,&nbsp;Solairaj Dhanasekaran ,&nbsp;Ruijie Luo ,&nbsp;Jun Li ,&nbsp;Lina Zhao ,&nbsp;Hongyin Zhang","doi":"10.1016/j.postharvbio.2025.113556","DOIUrl":"10.1016/j.postharvbio.2025.113556","url":null,"abstract":"<div><div>Postharvest eggplants are highly vulnerable to pathogen invasion, which results in quality deterioration and economic losses. Previous research has shown that <em>Bacillus velezensis</em> significantly reduced the soft rot occurrence of eggplants through producing cyclic lipopeptides and improving reactive oxygen species (ROS) scavenging capability of eggplants. In the present study, the mechanisms involved in soft rot management by <em>B. velezensis</em> were explored in depth, and a broad-spectrum biocontrol efficacy of this antagonistic bacterium against other major postharvest diseases of eggplants was further investigated. The results exhibited that <em>B. velezensis</em> has the ability to inhibit critical postharvest pathogens of eggplants, including <em>Botrytis cinerea</em> and <em>Alternaria alternate</em>. More importantly, this antagonistic bacterium decreased natural decay rate of eggplants from 72.25 % to 31.5 %. It suggested that this antagonist possessed extensive control effectiveness against primary diseases of postharvest eggplants. The effective biofilm formation of <em>B. velezensis</em> facilitated its colonization on fruit, which contributed to the biocontrol of postharvest diseases in eggplants. The exploration based on biochemical and transcriptomic analysis revealed that <em>B. velezensis</em> application resulted in the up-regulation of genes involved in Ca²⁺ signaling, plant hormone signaling, and bacterial AvrRpt2-mediated signaling in eggplants. Further, it triggered the expression of important genes involved in ROS scavenging and other defense responses, and boosted the activities of key defensive enzymes. Besides, <em>B. velezensis</em> application activated the genes involved in synthesizing resistance-associated secondary metabolites, such as lignins, flavonoids and anthocyanins, which align with the increased accumulation of these metabolites observed in eggplants. Collectively, the resistance against soft rot was stimulated in eggplants. This study offers valuable insights into the complex mechanisms by which antagonists like <em>B. velezensis</em> control postharvest diseases of eggplants and paves the way for developing strategies to control postharvest diseases in eggplants.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113556"},"PeriodicalIF":6.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential low oxygen response and transcriptomic shifts drive fresh-cut lettuce deterioration in modified atmosphere packaging","authors":"Hui Peng ,&nbsp;Dean O. Lavelle ,&nbsp;Maria Jose Truco ,&nbsp;Richard W. Michelmore ,&nbsp;Ivan Simko","doi":"10.1016/j.postharvbio.2025.113571","DOIUrl":"10.1016/j.postharvbio.2025.113571","url":null,"abstract":"<div><div>Fresh-cut lettuce (<em>Lactuca sativa</em>) requires modified atmosphere packaging (MAP) with low oxygen (&lt; 3 % O₂) to prevent enzymatic discoloration. However, some accessions exhibit rapid deterioration under these low oxygen conditions, resulting in significant product loss. This study investigated the mechanisms of this heritable deterioration, linked to the <em>qSL4</em> locus, by examining respiration and transcriptomic responses. We found that rapid deterioration is associated with continued, albeit reduced, respiration under very low oxygen levels (&lt;1 % O₂), with minimal effects from CO₂ and ethylene. Deterioration differences lessened at higher O₂ levels (∼20 %), emphasizing oxygen's critical role. RNA-seq analysis of slow (Salinas 88) and rapid (La Brillante) deteriorating cultivars revealed distinct early transcriptomic shifts under MAP. La Brillante upregulated more genes (1837) and downregulated fewer (1735) than Salinas 88 (1185 upregulated, 2367 downregulated), indicating higher metabolic activity in rapid deteriorating cultivar. Notably, glycolysis and electron transport chain (ETC) genes showed differential expression; ATP-consuming enzymes (fructokinase, hexokinase) and ETC complexes I and IV were upregulated in La Brillante but downregulated in Salinas 88. Conversely, Salinas 88 exhibited higher expression of alternative oxidase (AOX) genes, suggesting ATP conservation and reduced oxygen use. These findings indicate that slow-deteriorating genotypes maintain quality under low oxygen by limiting sugar and ATP consumption, while rapid deteriorating genotypes sustain higher metabolic activity, accelerating deterioration and likely shifting towards fermentation. This study provides insight into the early stages of lettuce deterioration and highlight the importance of low oxygen tolerance and metabolic adjustment capacity, setting the stage for future genomic research and functional studies on postharvest respiration and fermentation.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"227 ","pages":"Article 113571"},"PeriodicalIF":6.4,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous spermidine promotes the formation of the closing layer at potato tuber wounds by inducing polyamine synthesis and phenylpropanoid metabolism","authors":"Ying Wang ,&nbsp;Ruirui Yang ,&nbsp;Qihui Wang ,&nbsp;Pengdong Xie ,&nbsp;Xiaojing Wang ,&nbsp;Yongcai Li ,&nbsp;Dov Prusky ,&nbsp;Ye Han ,&nbsp;Yang Bi","doi":"10.1016/j.postharvbio.2025.113572","DOIUrl":"10.1016/j.postharvbio.2025.113572","url":null,"abstract":"<div><div>Spermidine (Spd) is an essential polyamine in plants. Although there are reports that exogenous Spd can enhance the synthesis of endogenous polyamines and induce plant resistance, it remains unknown whether exogenous Spd affects the formation of the potato tuber wound closing layers by modulating polyamine synthesis and phenylpropanoid metabolism. This study found that Spd enhanced the gene expression and activity of arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (SAMDC), leading to an increase in the levels of Put, Spd, and Spm. Additionally, Spd increased the activity of phenylalanine ammonia-lyase (PAL), 4-coumaroyl-CoA ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD), and elevated the levels of five phenolic acids and three lignin monomers. Furthermore, Spd increased the gene expression and activity of peroxidase (POD), diamine oxidase (DAO), and polyamine oxidase (PAO), resulting in an increase in the level of H₂O₂. The aforementioned phenolic acids and lignin monomers are oxidized and cross-linked under the combined action of POD and H₂O₂ to form suberin polyphenols (SPP) and lignin, which are deposited on the wound surface. The competitive inhibitor D-arginine (D-Arg) from ADC and the specific inhibitor guazatine (GZ) from PAO can delay the deposition of SPP and lignin by inhibiting polyamine synthesis, reducing the levels of phenolic acids and lignin monomers, and inhibiting POD activity and H₂O₂ production. In conclusion, exogenous Spd can promote the deposition of the closing layer on potato tuber wounds by promoting endogenous polyamine synthesis and phenylpropanoid metabolism.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"226 ","pages":"Article 113572"},"PeriodicalIF":6.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}