Pub Date : 2025-02-18DOI: 10.1016/j.postharvbio.2025.113462
Lijun Ling , Rongxiu Mo , Wenyue Zhang , Yijuan Jiang , Fanjin Kong , Lijun Feng , Yao Li , Rui Yue , Yongpeng Zhou
The antifungal activity of six microbial-derived volatile organic compounds (VOCs) [ 3-hepten-2-one (Hep), cis-3-octen-1-ol, 3-methylvalerate, isovalerate, 1-octen-3-ol (Oct), and 2-methylbutyrate] against Aspergillus flavus was investigated by determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). The results demonstrated that all 15 binary combinations of the VOCs presented additive effects against A. flavus. Among them, the combination of Hep and Oct, refers to COH, with concentrations of 2 μL L−1 and 2.5 μL L−1, respectively, displayed the most potent antifungal effect with the lowest concentration, in contrast to the individual MIC of Hep or Oct, which were reduced to 1/8 and 1/2. COH can induce membrane lipid peroxidation, enhance cell membrane permeability, and cause leakage of nucleic acids and proteins within the cell, and 1-octen-3-ol might play a leading role. Finally, applying COH at a concentration of 1/2 MICOct + 1/4 MICHep could effectively restrain the spread of A. flavus on wolfberries and exhibit excellent preservation effects. This study validates the antifungal effect of COH and may serve as a natural alternative to antimicrobial agents for post-harvest agricultural products.
{"title":"Unravelling the inhibition mechanism of 1-octene-3-ol combined with 3-heptene-2-one on Aspergillus flavus and its application in the preservation of wolfberries","authors":"Lijun Ling , Rongxiu Mo , Wenyue Zhang , Yijuan Jiang , Fanjin Kong , Lijun Feng , Yao Li , Rui Yue , Yongpeng Zhou","doi":"10.1016/j.postharvbio.2025.113462","DOIUrl":"10.1016/j.postharvbio.2025.113462","url":null,"abstract":"<div><div>The antifungal activity of six microbial-derived volatile organic compounds (VOCs) [ 3-hepten-2-one (Hep), cis-3-octen-1-ol, 3-methylvalerate, isovalerate, 1-octen-3-ol (Oct), and 2-methylbutyrate] against <em>Aspergillus flavus</em> was investigated by determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI). The results demonstrated that all 15 binary combinations of the VOCs presented additive effects against <em>A. flavus</em>. Among them, the combination of Hep and Oct, refers to C<sub>OH</sub>, with concentrations of 2 μL L<sup>−1</sup> and 2.5 μL L<sup>−1</sup>, respectively, displayed the most potent antifungal effect with the lowest concentration, in contrast to the individual MIC of Hep or Oct, which were reduced to 1/8 and 1/2. C<sub>OH</sub> can induce membrane lipid peroxidation, enhance cell membrane permeability, and cause leakage of nucleic acids and proteins within the cell, and 1-octen-3-ol might play a leading role. Finally, applying C<sub>OH</sub> at a concentration of 1/2 MIC<sub>Oct</sub> + 1/4 MIC<sub>Hep</sub> could effectively restrain the spread of <em>A. flavus</em> on wolfberries and exhibit excellent preservation effects. This study validates the antifungal effect of C<sub>OH</sub> and may serve as a natural alternative to antimicrobial agents for post-harvest agricultural products.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113462"},"PeriodicalIF":6.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.postharvbio.2025.113453
Tanjima Akter , Mohammad Akbar Faqeerzada , Yena Kim , Muhammad Fahri Reza Pahlawan , Umuhoza Aline , Haeun Kim , Hangi Kim , Byoung-Kwan Cho
Hyperspectral imaging (HSI) has emerged as a highly effective, non-destructive technique for detecting external and subsurface defects in agricultural products, offering rapid and accurate quality assessment. This study employed an advanced HSI system operating in the 400–2500 nm range, encompassing visible near-infrared (VNIR) and short-wave infrared (SWIR) wavelengths, to identify multiple defects in apples and pears. Specifically, this research focused on three key defect types, bruises, scars, and diseases, while addressing challenges related to defect size, shape, and severity variations. To enhance the spectral variability and improve detection accuracy, apples and pears were manually bruised in varying sizes, simulating both the early and advanced stages of bruising. These samples were analyzed at regular intervals over two days, capturing the progression of defect characteristics. This process yielded over 8300 spectral data points from carefully selected regions of interest (ROIs), providing a comprehensive dataset for analysis. Three multivariate models, linear discriminant analysis (LDA), support vector machines (SVM), and partial least squares discriminant analysis (PLS-DA), were employed to classify normal and defective fruit. Among these, the PLS-DA model demonstrated the highest performance, achieving validation classification accuracies of 97.5 % for apples and 100 % for pears in the VNIR range and 98 % for apples and 99.9 % for pears in the SWIR range. Moreover, successive projection algorithms (SPA) were employed for optimal band selection, and the obtained SPA beta coefficients were derived from the high-performing models and generated chemical visualization maps, enabling precise localization and detailed characterization of defects. By combining rapid and accurate defect identification with comprehensive flaw characterization, this study provides a practical and efficient framework for industrial post-harvest quality control, ultimately enhancing high-value agricultural products' marketability and storage stability.
{"title":"Hyperspectral imaging with multivariate analysis for detection of exterior flaws for quality evaluation of apples and pears","authors":"Tanjima Akter , Mohammad Akbar Faqeerzada , Yena Kim , Muhammad Fahri Reza Pahlawan , Umuhoza Aline , Haeun Kim , Hangi Kim , Byoung-Kwan Cho","doi":"10.1016/j.postharvbio.2025.113453","DOIUrl":"10.1016/j.postharvbio.2025.113453","url":null,"abstract":"<div><div>Hyperspectral imaging (HSI) has emerged as a highly effective, non-destructive technique for detecting external and subsurface defects in agricultural products, offering rapid and accurate quality assessment. This study employed an advanced HSI system operating in the 400–2500 nm range, encompassing visible near-infrared (VNIR) and short-wave infrared (SWIR) wavelengths, to identify multiple defects in apples and pears. Specifically, this research focused on three key defect types, bruises, scars, and diseases, while addressing challenges related to defect size, shape, and severity variations. To enhance the spectral variability and improve detection accuracy, apples and pears were manually bruised in varying sizes, simulating both the early and advanced stages of bruising. These samples were analyzed at regular intervals over two days, capturing the progression of defect characteristics. This process yielded over 8300 spectral data points from carefully selected regions of interest (ROIs), providing a comprehensive dataset for analysis. Three multivariate models, linear discriminant analysis (LDA), support vector machines (SVM), and partial least squares discriminant analysis (PLS-DA), were employed to classify normal and defective fruit. Among these, the PLS-DA model demonstrated the highest performance, achieving validation classification accuracies of 97.5 % for apples and 100 % for pears in the VNIR range and 98 % for apples and 99.9 % for pears in the SWIR range. Moreover, successive projection algorithms (SPA) were employed for optimal band selection, and the obtained SPA beta coefficients were derived from the high-performing models and generated chemical visualization maps, enabling precise localization and detailed characterization of defects. By combining rapid and accurate defect identification with comprehensive flaw characterization, this study provides a practical and efficient framework for industrial post-harvest quality control, ultimately enhancing high-value agricultural products' marketability and storage stability.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113453"},"PeriodicalIF":6.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-17DOI: 10.1016/j.postharvbio.2025.113447
Lingzhen Zeng , Yueying Sang , Shucheng Li , Mengshi Lin , Yifen Lin , Hui Wang , Yihui Chen , Wangjin Lu , Zhongqi Fan , Hetong Lin
To understand the underlying mechanism of the organic acid accumulation of postharvest longan fruit, integrative analyses of transcriptome and metabolome were performed in the fruit of two longan varieties (‘Fuyan’ and ‘Dongbi’) with different storability. Compared with ‘Dongbi’ longan, the ‘Fuyan’ cultivar exhibited higher fruit respiration rate, pulp breakdown index, and TA content, but a lower TSS content during storage. Targeted metabolomic analysis revealed that, in comparison to ‘Dongbi’ longan, during storage, ‘Fuyan’ longan accumulated higher levels of four organic acids, including 3-phenyllactic acid, lactate, succinic acid, and oxaloacetate. Transcriptomic analysis showed that DEGs between ‘Fuyan’ and ‘Dongbi’ longans were primarily enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis, and the TCA cycle. Additionally, WGCNA identified hub genes involved in these metabolic pathways. RT-qPCR analysis indicated that the expression of related genes (DlLDH, DlACL, DlPEPC, DlMDH, DlAST and DlSCS) was upregulated in ‘Fuyan’ longan compared with ‘Dongbi’ longan during storage. These findings suggest that the increase in longan pulp acidity is attributed to organic acid accumulation and an enhanced respiration metabolism, resulting in the poor storability of ‘Fuyan’ longan during storage.
{"title":"Integrative transcriptomic and metabolomic analyses reveal the mechanisms behind acidity differences in the pulp of fresh longan cv. ‘Fuyan’ and ‘Dongbi’ during storage","authors":"Lingzhen Zeng , Yueying Sang , Shucheng Li , Mengshi Lin , Yifen Lin , Hui Wang , Yihui Chen , Wangjin Lu , Zhongqi Fan , Hetong Lin","doi":"10.1016/j.postharvbio.2025.113447","DOIUrl":"10.1016/j.postharvbio.2025.113447","url":null,"abstract":"<div><div>To understand the underlying mechanism of the organic acid accumulation of postharvest longan fruit, integrative analyses of transcriptome and metabolome were performed in the fruit of two longan varieties (‘Fuyan’ and ‘Dongbi’) with different storability. Compared with ‘Dongbi’ longan, the ‘Fuyan’ cultivar exhibited higher fruit respiration rate, pulp breakdown index, and TA content, but a lower TSS content during storage. Targeted metabolomic analysis revealed that, in comparison to ‘Dongbi’ longan, during storage, ‘Fuyan’ longan accumulated higher levels of four organic acids, including 3-phenyllactic acid, lactate, succinic acid, and oxaloacetate. Transcriptomic analysis showed that DEGs between ‘Fuyan’ and ‘Dongbi’ longans were primarily enriched in respiration metabolism pathways, including glycolysis, gluconeogenesis, and the TCA cycle. Additionally, WGCNA identified hub genes involved in these metabolic pathways. RT-qPCR analysis indicated that the expression of related genes (<em>DlLDH</em>, <em>DlACL</em>, <em>DlPEPC</em>, <em>DlMDH</em>, <em>DlAST</em> and <em>DlSCS</em>) was upregulated in ‘Fuyan’ longan compared with ‘Dongbi’ longan during storage. These findings suggest that the increase in longan pulp acidity is attributed to organic acid accumulation and an enhanced respiration metabolism, resulting in the poor storability of ‘Fuyan’ longan during storage.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113447"},"PeriodicalIF":6.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.postharvbio.2025.113459
Caifeng Jiao , Jing Sun
Refrigeration process is often regarded as a traditional practice utilized to conserve nutritive value in postharvest horticultural products. As a result of susceptibility to low temperature environment, tomato fruit exhibit the development of chilling injury (CI) during refrigerated preservation, ultimately constraining marketability. Methyl salicylate (MeSA) application attenuated the CI in tomatoes. The MeSA-dipped fruit possessed the compromised reactive oxygen species (ROS) generation. MeSA application was conducive to improve the transcription level of SlNAC1. The SlNAC1-OE fruit displayed the increase in cold resistance, accompanied by the diminished amount of ROS. In contrast, the lower cold resistance and larger amount of ROS were observed in the SlNAC1-silenced fruit. MeSA application conferred the boosted transcription level of nucleoredoxin 2 (SlNRX2). Moreover, the transcription abundances of SlNRX2 increased by overexpression of SlNAC1, but decreased by RNAi of SlNAC1. It was substantiated that SlNAC1 transcriptionally promoted the SlNRX2 expression by targeting the NAC binding motif in its promoter region afterwards. Collectively, SlNAC1 was involved in the MeSA application-weakened sensitivity to low temperature stress by transactivating SlNRX2 in tomato fruit.
{"title":"SlNAC1 mediates MeSA application-promoted cold resistance by improving transcription of SlNRX2 in tomato fruit","authors":"Caifeng Jiao , Jing Sun","doi":"10.1016/j.postharvbio.2025.113459","DOIUrl":"10.1016/j.postharvbio.2025.113459","url":null,"abstract":"<div><div>Refrigeration process is often regarded as a traditional practice utilized to conserve nutritive value in postharvest horticultural products. As a result of susceptibility to low temperature environment, tomato fruit exhibit the development of chilling injury (CI) during refrigerated preservation, ultimately constraining marketability. Methyl salicylate (MeSA) application attenuated the CI in tomatoes. The MeSA-dipped fruit possessed the compromised reactive oxygen species (ROS) generation. MeSA application was conducive to improve the transcription level of <em>SlNAC1.</em> The <em>SlNAC1</em>-OE fruit displayed the increase in cold resistance, accompanied by the diminished amount of ROS. In contrast, the lower cold resistance and larger amount of ROS were observed in the <em>SlNAC1</em>-silenced fruit. MeSA application conferred the boosted transcription level of nucleoredoxin 2 (<em>SlNRX2</em>). Moreover, the transcription abundances of <em>SlNRX2</em> increased by overexpression of <em>SlNAC1</em>, but decreased by RNAi of <em>SlNAC1</em>. It was substantiated that SlNAC1 transcriptionally promoted the <em>SlNRX2</em> expression by targeting the NAC binding motif in its promoter region afterwards. Collectively, SlNAC1 was involved in the MeSA application-weakened sensitivity to low temperature stress by transactivating <em>SlNRX2</em> in tomato fruit.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113459"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.postharvbio.2025.113458
Ranran Xu , Wanqing Liu , Jiahua Zhou , Shuaiqi Zhang , Lihua Jiang , Bangdi Liu , Baogang Wang
Postharvest diseases caused by fungal infections in nectarine fruit seriously affect its commodity value and economic benefits. The effects and mechanisms of application of amino acid derivative ectoine against decay mold disease on postharvest nectarine fruit were evaluated. Ectoine remarkably reduced the occurrence of brown rot in nectarines after inoculation with Monilinia fructicola, which was most likely achieved by enhancing the resistance of the fruit. The expression of genes such as phenylalanine ammonia-lyase in ethylene transduction pathway were downregulated and the expressions of jasmonic acid and salicylic acid were upregulated with ectoine treatment. Moreover, the activities of key enzymes such as β-1,3-glucanase and superoxide dismutase for antibacterial and antioxidation activities, respectively, were enhanced. Phenylpropane biosynthesis was then activated and antioxidant capacity was enhanced. As a result, ectoine improved the resistance of nectarine fruit against fungal diseases. This study provides a new and promising approach to reduce postharvest decay of nectarine.
{"title":"Ectoine inhibits postharvest decay of nectarine fruit by strengthening fruit resistance","authors":"Ranran Xu , Wanqing Liu , Jiahua Zhou , Shuaiqi Zhang , Lihua Jiang , Bangdi Liu , Baogang Wang","doi":"10.1016/j.postharvbio.2025.113458","DOIUrl":"10.1016/j.postharvbio.2025.113458","url":null,"abstract":"<div><div>Postharvest diseases caused by fungal infections in nectarine fruit seriously affect its commodity value and economic benefits. The effects and mechanisms of application of amino acid derivative ectoine against decay mold disease on postharvest nectarine fruit were evaluated. Ectoine remarkably reduced the occurrence of brown rot in nectarines after inoculation with <em>Monilinia fructicola</em>, which was most likely achieved by enhancing the resistance of the fruit. The expression of genes such as phenylalanine ammonia-lyase in ethylene transduction pathway were downregulated and the expressions of jasmonic acid and salicylic acid were upregulated with ectoine treatment. Moreover, the activities of key enzymes such as β-1,3-glucanase and superoxide dismutase for antibacterial and antioxidation activities, respectively, were enhanced. Phenylpropane biosynthesis was then activated and antioxidant capacity was enhanced. As a result, ectoine improved the resistance of nectarine fruit against fungal diseases. This study provides a new and promising approach to reduce postharvest decay of nectarine.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113458"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-16DOI: 10.1016/j.postharvbio.2025.113457
Chenchen Wu , Chuanlong Men , Yaqian Wang , Tingting Fan , Changhong Liu , Lei Zheng
Postharvest strawberry is susceptible to mechanical damage and fungal attack, which poses serious storage challenges. In this study, senescence of strawberry fruit was successfully delayed using 0.25, 0.5 and 1 g L−1 α-lipoic acid (α-LA), and microbial proliferation was effectively inhibited during the storage. In particular, the treatment at with 0.5 g L−1 α-LA was the most effective, significantly reducing the decay rate and weight loss, while sustaining the firmness, TSS content and color. Strawberry fruit treated with α-LA showed higher total phenolics, total flavonoids, total anthocyanin, ascorbic acid, and individual phenolic compounds content compared with the control. α-LA attenuated strawberry membrane lipid peroxidation by inhibiting MDA and H2O2 production, and increasing antioxidant capacity. Transcriptomic analysis revealed significant enrichment of genes related to phenylpropanoid biosynthesis, and the transcription factors FaMYC2 and FaMYB308 might act as negative regulators in phenolic metabolism. In addition, α-LA remarkably enhanced the expression of key genes related to phenolic metabolism and anthocyanin synthesis. In summary, α-LA maintained quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry.
{"title":"α-lipoic acid maintains quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry","authors":"Chenchen Wu , Chuanlong Men , Yaqian Wang , Tingting Fan , Changhong Liu , Lei Zheng","doi":"10.1016/j.postharvbio.2025.113457","DOIUrl":"10.1016/j.postharvbio.2025.113457","url":null,"abstract":"<div><div>Postharvest strawberry is susceptible to mechanical damage and fungal attack, which poses serious storage challenges. In this study, senescence of strawberry fruit was successfully delayed using 0.25, 0.5 and 1 g L<sup>−1</sup> α-lipoic acid (α-LA), and microbial proliferation was effectively inhibited during the storage. In particular, the treatment at with 0.5 g L<sup>−1</sup> α-LA was the most effective, significantly reducing the decay rate and weight loss, while sustaining the firmness, TSS content and color. Strawberry fruit treated with α-LA showed higher total phenolics, total flavonoids, total anthocyanin, ascorbic acid, and individual phenolic compounds content compared with the control. α-LA attenuated strawberry membrane lipid peroxidation by inhibiting MDA and H<sub>2</sub>O<sub>2</sub> production, and increasing antioxidant capacity. Transcriptomic analysis revealed significant enrichment of genes related to phenylpropanoid biosynthesis, and the transcription factors FaMYC2 and FaMYB308 might act as negative regulators in phenolic metabolism. In addition, α-LA remarkably enhanced the expression of key genes related to phenolic metabolism and anthocyanin synthesis. In summary, α-LA maintained quality by modulating phenolic metabolism and antioxidant capacity in postharvest strawberry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113457"},"PeriodicalIF":6.4,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-15DOI: 10.1016/j.postharvbio.2025.113445
Yunyun Weng , Sitong Wang , Tongfei Niu , Yuying Li , Chengwei Song , Qi Guo , Linfeng Chen , Shaodan Liu , Xiaogai Hou , Lili Guo
Vase-inserted cut tree peony flowers have a short viewing period and research focusing on prolonging the flowering duration is limited. This study investigated the effects of spermidine (Spd) on tree peony cut flowers inserted in vases containing different concentrations of Spd solutions. Paeonia suffruticosa ‘Luoyang Hong’ cut flowers at the full blooming stage were used as the test material, and floral aroma, phenotypic characteristics, and physiological indices were selected as the evaluation indicators. The results indicated that a concentration of 400 μmol·L−1 Spd significantly increased the contents of terpenoids, ketones, and alcohols, while 5 μmol·L−1 Spd significantly elevated the levels of hydrocarbons and benzene. The aldehyde content in the vase-inserted tree peony cut flowers was significantly enhanced after the addition of 300 μmol·L−1 Spd. Moreover, 5 μmol·L−1 Spd significantly improved the fresh weight, flower diameter, and flower height, prolonged the flowering period, increased the superoxide dismutase, peroxidase activities, and soluble protein content, and decreased the activities of ACC oxidase, ACC synthetase, and malondialdehyde (MDA). In conclusion, the addition of 5 μmol·L−1 Spd effectively enhanced the contents of floral phenylcyclic compounds, maintained the ornamental quality, and delayed the senescence of vase-inserted cut tree peony flowers.
{"title":"Effect of spermidine on the quality of vase-inserted Paeonia suffruticosa ‘Luoyang Hong’","authors":"Yunyun Weng , Sitong Wang , Tongfei Niu , Yuying Li , Chengwei Song , Qi Guo , Linfeng Chen , Shaodan Liu , Xiaogai Hou , Lili Guo","doi":"10.1016/j.postharvbio.2025.113445","DOIUrl":"10.1016/j.postharvbio.2025.113445","url":null,"abstract":"<div><div>Vase-inserted cut tree peony flowers have a short viewing period and research focusing on prolonging the flowering duration is limited. This study investigated the effects of spermidine (Spd) on tree peony cut flowers inserted in vases containing different concentrations of Spd solutions. <em>Paeonia suffruticosa</em> ‘Luoyang Hong’ cut flowers at the full blooming stage were used as the test material, and floral aroma, phenotypic characteristics, and physiological indices were selected as the evaluation indicators. The results indicated that a concentration of 400 μmol·L<sup>−1</sup> Spd significantly increased the contents of terpenoids, ketones, and alcohols, while 5 μmol·L<sup>−1</sup> Spd significantly elevated the levels of hydrocarbons and benzene. The aldehyde content in the vase-inserted tree peony cut flowers was significantly enhanced after the addition of 300 μmol·L<sup>−1</sup> Spd. Moreover, 5 μmol·L<sup>−1</sup> Spd significantly improved the fresh weight, flower diameter, and flower height, prolonged the flowering period, increased the superoxide dismutase, peroxidase activities, and soluble protein content, and decreased the activities of ACC oxidase, ACC synthetase, and malondialdehyde (MDA). In conclusion, the addition of 5 μmol·L<sup>−1</sup> Spd effectively enhanced the contents of floral phenylcyclic compounds, maintained the ornamental quality, and delayed the senescence of vase-inserted cut tree peony flowers.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113445"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-15DOI: 10.1016/j.postharvbio.2025.113451
Fan Liu , Xueli Sun , Jia He , Jingjing Shan , Ou Sheng , Tongxin Dou , Yaoyao Li , Weidi He , Qiaosong Yang , Chunhua Hu , Huijun Gao , Xinxiang Peng , Ganjun Yi , Fangcheng Bi
Fruit ripening is a complex process involving physiological and metabolic changes that influence fruit color, flavor, aroma, and texture, which is modulated by various hormones and key genes. NAC with transmembrane motif-like (NTL) transcription factors belong to the NAC gene family with transmembrane motifs at their C-terminus and play important roles in plant growth, development, and stress responses. However, the role of NTL during fruit ripening remains enigmatic. Here, we identified a NTL gene, MaNAC169, that was regulated by ethylene during banana fruit ripening. MaNAC169 contained a conserved TM in its far C-terminal region and localized in the nucleus and plasma membrane. Its active form, MaNAC169-ΔC4, acted as a transcriptional activator. Importantly, MaNAC169 directly bound to the promoters of MaACO1, MaEXP2, MaXET1, and MaGWD1 and positively regulated their gene expression. MaNAC169 also activated its own expression and could form a dimer with itself. Moreover, transient MaNAC169-ΔC4 overexpression in banana fruit promoted ripening by inducing target gene expression. Taken together, MaNAC169 positively modulated fruit ripening by activating the expression of ripening-associated genes. Our findings provide new insights into the transcriptional regulatory mechanisms involved in fruit ripening.
{"title":"Membrane-associated NAC transcription factor MaNAC169 is a positive regulator during banana fruit ripening","authors":"Fan Liu , Xueli Sun , Jia He , Jingjing Shan , Ou Sheng , Tongxin Dou , Yaoyao Li , Weidi He , Qiaosong Yang , Chunhua Hu , Huijun Gao , Xinxiang Peng , Ganjun Yi , Fangcheng Bi","doi":"10.1016/j.postharvbio.2025.113451","DOIUrl":"10.1016/j.postharvbio.2025.113451","url":null,"abstract":"<div><div>Fruit ripening is a complex process involving physiological and metabolic changes that influence fruit color, flavor, aroma, and texture, which is modulated by various hormones and key genes. NAC with transmembrane motif-like (NTL) transcription factors belong to the NAC gene family with transmembrane motifs at their C-terminus and play important roles in plant growth, development, and stress responses. However, the role of NTL during fruit ripening remains enigmatic. Here, we identified a NTL gene, MaNAC169, that was regulated by ethylene during banana fruit ripening. MaNAC169 contained a conserved TM in its far C-terminal region and localized in the nucleus and plasma membrane. Its active form, MaNAC169-ΔC4, acted as a transcriptional activator. Importantly, MaNAC169 directly bound to the promoters of <em>MaACO1</em>, <em>MaEXP2</em>, <em>MaXET1</em>, and <em>MaGWD1</em> and positively regulated their gene expression. MaNAC169 also activated its own expression and could form a dimer with itself. Moreover, transient <em>MaNAC169-ΔC4</em> overexpression in banana fruit promoted ripening by inducing target gene expression. Taken together, MaNAC169 positively modulated fruit ripening by activating the expression of ripening-associated genes. Our findings provide new insights into the transcriptional regulatory mechanisms involved in fruit ripening.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113451"},"PeriodicalIF":6.4,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-13DOI: 10.1016/j.postharvbio.2025.113454
Qingbiao Xie , Limei Huang , Qianyi Xiao , Hongli Luo , Qiannan Wang , Bang An
Postharvest diseases, particularly anthracnose caused by Colletotrichum gloeosporioides, pose significant challenges to global fruit markets by reducing quality and marketability. Although traditional fungicides like Iprodione are commonly used for disease control, their effectiveness is increasingly limited by environmental concerns and the emergence of resistant fungal strains. This study investigated the potential of berberine, a natural alkaloid, to enhance the antifungal efficacy of Iprodione. In vitro assays showed that berberine significantly improved Iprodione’s inhibitory effects on fungal growth and conidia germ tube elongation. In vivo tests on apple fruit further validated these findings, showing significant reductions in lesion when berberine and Iprodione were applied together compared to either treatment alone. Western blot analysis revealed that the combined treatment significantly decreased protein phosphorylation in C. gloeosporioides, indicating enhanced dephosphorylation effects. Transcriptomic analysis revealed that berberine downregulates key detoxification and fungicide-resistance genes, such as cytochrome P450s, amidohydrolases, DyP-type peroxidase and membrane transporters, disrupting detoxification pathways and increasing Iprodione’s toxicity. Gene knockout studies indicated that ΔCgADH1 and ΔCgDyPOD1 mutants exhibited increased sensitivity to Iprodione, highlighting their roles in resistance mechanisms. These findings suggest that berberine can synergistically enhance Iprodione’s antifungal activity, providing a promising, eco-friendly strategy for controlling postharvest anthracnose while potentially reducing fungicide usage in the fruit industry.
{"title":"Berberine Synergistically Enhances Iprodione’s Antifungal Activity Against Colletotrichum gloeosporioides","authors":"Qingbiao Xie , Limei Huang , Qianyi Xiao , Hongli Luo , Qiannan Wang , Bang An","doi":"10.1016/j.postharvbio.2025.113454","DOIUrl":"10.1016/j.postharvbio.2025.113454","url":null,"abstract":"<div><div>Postharvest diseases, particularly anthracnose caused by <em>Colletotrichum gloeosporioides</em>, pose significant challenges to global fruit markets by reducing quality and marketability. Although traditional fungicides like Iprodione are commonly used for disease control, their effectiveness is increasingly limited by environmental concerns and the emergence of resistant fungal strains. This study investigated the potential of berberine, a natural alkaloid, to enhance the antifungal efficacy of Iprodione. <em>In vitro</em> assays showed that berberine significantly improved Iprodione’s inhibitory effects on fungal growth and conidia germ tube elongation. <em>In vivo</em> tests on apple fruit further validated these findings, showing significant reductions in lesion when berberine and Iprodione were applied together compared to either treatment alone. Western blot analysis revealed that the combined treatment significantly decreased protein phosphorylation in <em>C. gloeosporioides</em>, indicating enhanced dephosphorylation effects. Transcriptomic analysis revealed that berberine downregulates key detoxification and fungicide-resistance genes, such as cytochrome P450s, amidohydrolases, DyP-type peroxidase and membrane transporters, disrupting detoxification pathways and increasing Iprodione’s toxicity. Gene knockout studies indicated that Δ<em>CgADH1</em> and Δ<em>CgDyPOD1</em> mutants exhibited increased sensitivity to Iprodione, highlighting their roles in resistance mechanisms. These findings suggest that berberine can synergistically enhance Iprodione’s antifungal activity, providing a promising, eco-friendly strategy for controlling postharvest anthracnose while potentially reducing fungicide usage in the fruit industry.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113454"},"PeriodicalIF":6.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-12DOI: 10.1016/j.postharvbio.2025.113418
Yajun Wang , Shuang Min , Tian Gao , Canying Li , Yonghong Ge
This study was performed to explore the impact of atomized fumigation with basil essential oil (BEO) on the metabolism of reactive oxygen species, respiration, and energy, as well as quality parameters in blueberry fruit. Results demonstrated that BEO had minimal impact on surface color, significantly inhibited respiratory rate and ethylene release, while maintaining ascorbic acid, reducing sugar, soluble sugar, and reduced glutathione levels in blueberries. Moreover, BEO attenuated weight loss increase and inhibited hydrogen peroxide and malondialdehyde accumulation. BEO resulted in enhanced activities and gene expressions of ascorbate peroxidase, NADPH hydrogenase, superoxide dismutase, peroxidase, glutathione reductase, cytochrome C oxidase, hexokinase, glucose-phosphate isomerase, succinate dehydrogenase, and nicotinamide adenine dinucleotide kinase. Meanwhile, BEO up-regulated VcH+-ATPase and VcCa2+-ATPase expressions, as well as enhanced catalase, glucose-6-phosphate dehydrogenase, and polyphenol oxidase activities. Additionally, BEO reduced NAD/NADH levels, while increasing NADP/NADPH levels. In summary, BEO fumigation can enhance the antioxidant capacity, mitigate membrane peroxidation, modulate energy and respiration metabolism, thereby preserving the storage quality of blueberries.
{"title":"Atomized fumigation with basil essential oil regulated reactive oxygen metabolism and respiratory metabolism to maintain storage quality of blueberry fruit","authors":"Yajun Wang , Shuang Min , Tian Gao , Canying Li , Yonghong Ge","doi":"10.1016/j.postharvbio.2025.113418","DOIUrl":"10.1016/j.postharvbio.2025.113418","url":null,"abstract":"<div><div>This study was performed to explore the impact of atomized fumigation with basil essential oil (BEO) on the metabolism of reactive oxygen species, respiration, and energy, as well as quality parameters in blueberry fruit. Results demonstrated that BEO had minimal impact on surface color, significantly inhibited respiratory rate and ethylene release, while maintaining ascorbic acid, reducing sugar, soluble sugar, and reduced glutathione levels in blueberries. Moreover, BEO attenuated weight loss increase and inhibited hydrogen peroxide and malondialdehyde accumulation. BEO resulted in enhanced activities and gene expressions of ascorbate peroxidase, NADPH hydrogenase, superoxide dismutase, peroxidase, glutathione reductase, cytochrome C oxidase, hexokinase, glucose-phosphate isomerase, succinate dehydrogenase, and nicotinamide adenine dinucleotide kinase. Meanwhile, BEO up-regulated <em>VcH</em><sup><em>+</em></sup><em>-ATPase</em> and <em>VcCa</em><sup><em>2+</em></sup><em>-ATPase</em> expressions, as well as enhanced catalase, glucose-6-phosphate dehydrogenase, and polyphenol oxidase activities. Additionally, BEO reduced NAD/NADH levels, while increasing NADP/NADPH levels. In summary, BEO fumigation can enhance the antioxidant capacity, mitigate membrane peroxidation, modulate energy and respiration metabolism, thereby preserving the storage quality of blueberries.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"223 ","pages":"Article 113418"},"PeriodicalIF":6.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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