The ERF transcription factor ZbERF3 promotes ethylene-induced anthocyanin biosynthesis in Zanthoxylum bungeanum

IF 4.2 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Plant Science Pub Date : 2024-09-12 DOI:10.1016/j.plantsci.2024.112264

Shuangyu Zhang , Shen Liu , Yanshen Ren , Jie Zhang , Nuan Han , Cheng Wang , Dongmei Wang , Houhua Li

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Abstract

Ethylene regulates fruit ripening, and in Zanthoxylum bungeanum, fruit color deepened with increasing of ethylene during fruit ripening. However, the molecular mechanism of this physiological process was still unclear. In this study, through the combined analysis of transcriptome and metabolome, it was found that ethylene release was consistent with anthocyanin synthesis, and ethylene response factors (ERFs) were significantly related to anthocyanin biosynthesis during the fruit ripening of Z. bungeanum. Ethylene treatment significantly induced fruit coloration and promoted anthocyanin synthesis and the expression of ZbERF3. Furthermore, Yeast one-hybrid assays and Luciferase reporter assays demonstrated that ZbERF3 directly bound to the promoter of ZbMYB17 and transcriptionally activated its expression. What's more, it was demonstrated that ZbMYB17 directly bound to the promoter of ZbANS, promoting anthocyanin biosynthesis. Overall, this study revealed the mechanism of ERF and MYB synergistically regulating the coloration of Z. bungeanum fruit.

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ERF 转录因子 ZbERF3 促进乙烯诱导的花青素生物合成

乙烯能调节果实成熟，在 Zanthoxylum bungeanum 中，果实成熟过程中果实颜色随乙烯的增加而加深。然而，这一生理过程的分子机制尚不清楚。本研究通过转录组和代谢组的联合分析发现，乙烯释放与花青素合成一致，乙烯响应因子（ERFs）与花青素生物合成显著相关。乙烯处理能明显诱导果实着色，促进花青素合成和 ZbERF3 的表达。此外，酵母单杂交实验和荧光素酶报告实验表明，ZbERF3 直接与 ZbMYB17 的启动子结合并转录激活其表达。此外，研究还发现 ZbMYB17 与 ZbANS 的启动子直接结合，促进了花青素的生物合成。总之，本研究揭示了 ERF 和 MYB 协同调控文冠果着色的机制。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.