Pub Date : 2025-04-24DOI: 10.1016/j.plantsci.2025.112529
Liting Shen , Huizhen Huang , Daqi Yan , Yongsheng Ye , Jun Hu
The regulation of protein levels in plants is essential for improving agricultural productivity. Recent studies have explored inducible degradation systems in plants, with some showing promising advancements. This study introduces the NRDN degradation tag as a novel tool for regulating protein stability within the nucleus in Arabidopsis thaliana. Unlike traditional gene knockout methods, NRDN offers real-time, dynamic control over protein degradation, enabling precise studies of nuclear-localized proteins. This discovery provides a valuable tool for regulating protein stability in specific cellular compartments, which presents a versatile approach for dissecting complex cellular processes and offers broad applications in functional genomics and cellular research.
{"title":"NRDN: A novel nuclear degradation tag for targeted protein regulation in Arabidopsis","authors":"Liting Shen , Huizhen Huang , Daqi Yan , Yongsheng Ye , Jun Hu","doi":"10.1016/j.plantsci.2025.112529","DOIUrl":"10.1016/j.plantsci.2025.112529","url":null,"abstract":"<div><div>The regulation of protein levels in plants is essential for improving agricultural productivity. Recent studies have explored inducible degradation systems in plants, with some showing promising advancements. This study introduces the NRDN degradation tag as a novel tool for regulating protein stability within the nucleus in <em>Arabidopsis thaliana</em>. Unlike traditional gene knockout methods, NRDN offers real-time, dynamic control over protein degradation, enabling precise studies of nuclear-localized proteins. This discovery provides a valuable tool for regulating protein stability in specific cellular compartments, which presents a versatile approach for dissecting complex cellular processes and offers broad applications in functional genomics and cellular research.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112529"},"PeriodicalIF":4.2,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.plantsci.2025.112518
Jingwen Xiao , Wenxu Liu , Bicong Wu , Yuling Zhang , Sha Li , En Li
Root hairs anchor the plant in the soil, facilitating nutrient assimilation, water absorption, and interaction of plants with their environment. In legumes, they play a key role in the early infection of rhizobia. This review aimed to summarize the recent progress about the nodulation factor receptors on the root hair surface. It also discussed the importance of downstream signaling pathways of nodulation factor receptors and highlighted Rho of plants signaling pathway that controls infection thread polar growth and nodulation.
{"title":"Root hair: An important guest-meeting avenue for rhizobia in legume–Rhizobium symbiosis","authors":"Jingwen Xiao , Wenxu Liu , Bicong Wu , Yuling Zhang , Sha Li , En Li","doi":"10.1016/j.plantsci.2025.112518","DOIUrl":"10.1016/j.plantsci.2025.112518","url":null,"abstract":"<div><div>Root hairs anchor the plant in the soil, facilitating nutrient assimilation, water absorption, and interaction of plants with their environment. In legumes, they play a key role in the early infection of rhizobia. This review aimed to summarize the recent progress about the nodulation factor receptors on the root hair surface. It also discussed the importance of downstream signaling pathways of nodulation factor receptors and highlighted Rho of plants signaling pathway that controls infection thread polar growth and nodulation.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112518"},"PeriodicalIF":4.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-22DOI: 10.1016/j.plantsci.2025.112517
Xiaoqun Peng , Yilin Li , Jingmei Xu , Ying Zeng , Kun Li , Xiangyi Guo , Zikang Zhang , Xiaoyan Tang , Menglong Wang
Lectin receptor-like kinases (LecRKs) are a critical class of plant proteins that play essential roles in plant development as well as in responses to both biotic and abiotic stresses. In this study, we found that overexpression of the L-type Lectin receptor kinase gene OsLecRK-S.7 severely inhibits plant growth and triggers spontaneous cell death. Meanwhile, immune responses, including pathogenesis-related (PR) gene expression and reactive oxygen species (ROS) accumulation, were elevated in OsLecRK-S.7 overexpressing plants. Kinase inactivation experiments demonstrated that kinase activity was essential for OsLecRK-S.7-mediated constitutive immunity. Infection assays further demonstrated that overexpression of OsLecRK-S.7 enhances rice resistance to bacterial blight. Additionally, bimolecular fluorescence complementation (BiFC) and pull-down experiments identified interactions between OsLecRK-S.7 and receptor-like cytoplasmic kinases (RLCKs) OsRLCK118, OsRLCK185, and OsRLCK107 that are involved in immune signaling. These findings suggest that OsLecRK-S.7 is a significant regulator of plant immunity, likely promoting cell death and immune responses through its interactions with OsRLCK118, OsRLCK185, and OsRLCK107.
{"title":"Overexpression of the lectin receptor-like kinase gene OsLecRK-S.7 inhibits plant growth and enhances disease resistance in rice","authors":"Xiaoqun Peng , Yilin Li , Jingmei Xu , Ying Zeng , Kun Li , Xiangyi Guo , Zikang Zhang , Xiaoyan Tang , Menglong Wang","doi":"10.1016/j.plantsci.2025.112517","DOIUrl":"10.1016/j.plantsci.2025.112517","url":null,"abstract":"<div><div>Lectin receptor-like kinases (LecRKs) are a critical class of plant proteins that play essential roles in plant development as well as in responses to both biotic and abiotic stresses. In this study, we found that overexpression of the <span>L</span>-type Lectin receptor kinase gene <em>OsLecRK-S.7</em> severely inhibits plant growth and triggers spontaneous cell death. Meanwhile, immune responses, including pathogenesis-related (PR) gene expression and reactive oxygen species (ROS) accumulation, were elevated in <em>OsLecRK-S.7</em> overexpressing plants. Kinase inactivation experiments demonstrated that kinase activity was essential for OsLecRK-S.7-mediated constitutive immunity. Infection assays further demonstrated that overexpression of <em>OsLecRK-S.7</em> enhances rice resistance to bacterial blight. Additionally, bimolecular fluorescence complementation (BiFC) and pull-down experiments identified interactions between OsLecRK-S.7 and receptor-like cytoplasmic kinases (RLCKs) OsRLCK118, OsRLCK185, and OsRLCK107 that are involved in immune signaling. These findings suggest that OsLecRK-S.7 is a significant regulator of plant immunity, likely promoting cell death and immune responses through its interactions with OsRLCK118, OsRLCK185, and OsRLCK107.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112517"},"PeriodicalIF":4.2,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143876756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-21DOI: 10.1016/j.plantsci.2025.112519
Hanqiang Liu , Kaihong Hu , Yuxuan Ma , Liting Fu , Zeqiang Huang , Zhihui Cheng , Yuyan Sheng , Dandan Li , Yupeng Pan
Early senescence in plants significantly affects photosynthetic efficiency, crop yield, and overall plant vigor. In this study, we identified a spontaneous cucumber mutant, NW079, exhibiting premature leaf yellowing, reduced chlorophyll content, and impaired photosynthetic performance. To uncover the genetic basis of this phenotype, we generated F₂ mapping populations and employed bulked segregant analysis and fine mapping. These efforts led to the identification of a 5.5-kb long terminal repeat (LTR) retrotransposon insertion within the first exon of CsPHYB, a gene encoding phytochrome B. This insertion disrupted normal splicing and gave rise to two aberrant transcript variants: one containing a 261-bp LTR-derived sequence with premature stop codons, and the other harboring a 1,914-bp deletion due to exon skipping. Both variants are predicted to produce truncated, nonfunctional proteins. Functional analyses revealed that CsPHYB deficiency resulted in heightened sensitivity to varying light qualities and intensities, leading to pronounced leaf yellowing and reduced leaf area. RNA sequencing revealed widespread transcriptional reprogramming in NW079, with 580 differentially expressed genes (DEGs) implicated in heme metabolism, tetrapyrrole binding, and chloroplast development. These transcriptional disruptions were closely linked to the observed structural and functional abnormalities in chloroplasts. This study provides a molecular framework for understanding the early senescence in cucumber, offering valuable insights for breeding strategies aimed at improving crop resilience and productivity.
Keymessage
An LTR retrotransposon insertion in the first exon of CsPhyB disrupts its expression and splicing, leading to early leaf senescence in cucumber. This finding provides novel insights into the role of CsPHYB in chloroplast development and light signaling, offering valuable molecular markers and a target gene for cucumber breeding programs focused on enhancing yield and stress resilience.
{"title":"Identification and functional analysis of an LTR retrotransposon insertion in CsPHYB associated with early senescence in cucumber (Cucumis sativus L.)","authors":"Hanqiang Liu , Kaihong Hu , Yuxuan Ma , Liting Fu , Zeqiang Huang , Zhihui Cheng , Yuyan Sheng , Dandan Li , Yupeng Pan","doi":"10.1016/j.plantsci.2025.112519","DOIUrl":"10.1016/j.plantsci.2025.112519","url":null,"abstract":"<div><div><em>Early senescence</em> in plants significantly affects photosynthetic efficiency, crop yield, and overall plant vigor. In this study, we identified a spontaneous cucumber mutant, NW079, exhibiting premature leaf yellowing, reduced chlorophyll content, and impaired photosynthetic performance. To uncover the genetic basis of this phenotype, we generated F₂ mapping populations and employed bulked segregant analysis and fine mapping. These efforts led to the identification of a 5.5-kb long terminal repeat (LTR) retrotransposon insertion within the first exon of <em>CsPHYB</em>, a gene encoding phytochrome B. This insertion disrupted normal splicing and gave rise to two aberrant transcript variants: one containing a 261-bp LTR-derived sequence with premature stop codons, and the other harboring a 1,914-bp deletion due to exon skipping. Both variants are predicted to produce truncated, nonfunctional proteins. Functional analyses revealed that <em>CsPHYB</em> deficiency resulted in heightened sensitivity to varying light qualities and intensities, leading to pronounced leaf yellowing and reduced leaf area. RNA sequencing revealed widespread transcriptional reprogramming in NW079, with 580 differentially expressed genes (DEGs) implicated in heme metabolism, tetrapyrrole binding, and chloroplast development. These transcriptional disruptions were closely linked to the observed structural and functional abnormalities in chloroplasts. This study provides a molecular framework for understanding the <em>early senescence</em> in cucumber, offering valuable insights for breeding strategies aimed at improving crop resilience and productivity.</div><div><strong>Keymessage</strong></div><div>An LTR retrotransposon insertion in the first exon of <em>CsPhyB</em> disrupts its expression and splicing, leading to early leaf senescence in cucumber. This finding provides novel insights into the role of <em>CsPHYB</em> in chloroplast development and light signaling, offering valuable molecular markers and a target gene for cucumber breeding programs focused on enhancing yield and stress resilience.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112519"},"PeriodicalIF":4.2,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-20DOI: 10.1016/j.plantsci.2025.112521
Pan Zhao , Yanhong Zhang , Zhengliang Sun , Baohui Cheng , Liangzhe Meng , Tayeb Muhammad , Yuhe Yao , Muhammad Mehran Abbas , Xiangqiang Zhan , Fei Zhang , Yan Liang
Tomato stigma exsertion is an important trait in positional sterility genotypes, which can eliminate the need for manual emasculation and promote hybrid production. In this study, we discovered a new tomato accession, J59, with a stably inherited stigma exsertion trait. To explore the regulatory genes of the stigma exsertion trait, J59 and inserted stigma genotypes M82 were crossed to obtain mapping populations. Through three years mapping, a quantitative trait locus (QTL) associated with the stigma exsertion trait was narrowed down to a 52.635 kb interval on chromosome 2, Solyc02g087270 was identified as the candidate gene responsible for this trait, named SlSE2.2. This gene encoded an F-box protein of the FBA subfamily. Sequences analysis revealed that an 11 bp deletion occurred in the first exon of SlSE2.2 in J59, resulting in premature termination of translation. Subcellular localization revealed that SlSE2.2 is located to the nucleus. Knockout of SlSE2.2 increased anther and style lengths, which reduced the values of anther length minus pistil length, changing the relative length of anthers and pistils, called stigma exsertion degree, whereas, overexpression of SlSE2.2 showed the opposite phenotype. Hormone levels analysis revealed that SlSE2.2 negatively modulated IAA, ETH, and JA levels and positively modulated ABA content. Transcriptomic analysis showed that SlSE2.2 affected the expression of SlIAA19, SlIAA36, SlETR6, SlJAZ, and SlSnRK2 related to the hormone signal transduction. This study identified the important role of a new gene, SlSE2.2, which provided a helpful insight to explore the regulatory mechanisms of stigma exsertion degree in tomato.
{"title":"A novel F-box gene, SlSE2.2, is responsible for the stigma exsertion degree in tomato (Solanum lycopersicum)","authors":"Pan Zhao , Yanhong Zhang , Zhengliang Sun , Baohui Cheng , Liangzhe Meng , Tayeb Muhammad , Yuhe Yao , Muhammad Mehran Abbas , Xiangqiang Zhan , Fei Zhang , Yan Liang","doi":"10.1016/j.plantsci.2025.112521","DOIUrl":"10.1016/j.plantsci.2025.112521","url":null,"abstract":"<div><div>Tomato stigma exsertion is an important trait in positional sterility genotypes, which can eliminate the need for manual emasculation and promote hybrid production. In this study, we discovered a new tomato accession, J59, with a stably inherited stigma exsertion trait. To explore the regulatory genes of the stigma exsertion trait, J59 and inserted stigma genotypes M82 were crossed to obtain mapping populations. Through three years mapping, a quantitative trait locus (QTL) associated with the stigma exsertion trait was narrowed down to a 52.635 kb interval on chromosome 2, <em>Solyc02g087270</em> was identified as the candidate gene responsible for this trait, named <em>SlSE2.2</em>. This gene encoded an F-box protein of the FBA subfamily. Sequences analysis revealed that an 11 bp deletion occurred in the first exon of <em>SlSE2.2</em> in J59, resulting in premature termination of translation. Subcellular localization revealed that SlSE2.2 is located to the nucleus. Knockout of <em>SlSE2.2</em> increased anther and style lengths, which reduced the values of anther length minus pistil length, changing the relative length of anthers and pistils, called stigma exsertion degree, whereas, overexpression of <em>SlSE2.2</em> showed the opposite phenotype. Hormone levels analysis revealed that <em>SlSE2.2</em> negatively modulated IAA, ETH, and JA levels and positively modulated ABA content. Transcriptomic analysis showed that <em>SlSE2.2</em> affected the expression of <em>SlIAA19</em>, <em>SlIAA36</em>, <em>SlETR6</em>, <em>SlJAZ</em>, and <em>SlSnRK2</em> related to the hormone signal transduction. This study identified the important role of a new gene, <em>SlSE2.2</em>, which provided a helpful insight to explore the regulatory mechanisms of stigma exsertion degree in tomato.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112521"},"PeriodicalIF":4.2,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-19DOI: 10.1016/j.plantsci.2025.112495
Yuchen Wei , Jingfang Shi , Xueyi Xie , Feng Zhang , Huizhen Dong , Yaoyao Li , Fangcheng Bi , Xiaosan Huang , Tongxin Dou
Banana, a globally cultivated fruit, faces significant constraints in distribution and sustainable production due to drought stress. This study investigated drought tolerance in Cavendish bananas using RNA-seq time-course analysis and molecular biology experiments. Plants were subjected to dehydration treatments, and physiological indicators such as electrolyte leakage, proline content, malonaldehyde, peroxidase activity, and hydrogen peroxide content were assessed. RNA-Seq and qRT-PCR were used to analyze transcriptional changes under drought. Weighted gene co-expression network (WGCNA) analysis identified thousands of differentially expressed genes (DEGs) at different time points, with a core set of 2660 DEGs consistently identified. KEGG enrichment analysis revealed MaGME777, a glycolysis/gluconeogenesis gene, as a potential drought resistance regulator. Virus-mediated gene silencing (VIGS) of MaGME777 reduced drought tolerance in bananas. Yeast one-hybrid (Y1H) and luciferase reporter assays demonstrated that the transcription factor MabHLH770 directly binds and activates the MaGME777 promoter. VIGS downregulation of MabHLH770 also reduced drought tolerance. In conclusion, this study revealed that MabHLH770 is a positive regulator of drought stress, by targeting MaGME777 promoter and activating their expression to enhance drought tolerance. These findings provide a foundation for developing drought-resistant banana cultivars through molecular breeding approaches.
{"title":"Transcriptome sequence reveal the roles of MaGME777 and MabHLH770 in drought tolerance in Musa acuminata","authors":"Yuchen Wei , Jingfang Shi , Xueyi Xie , Feng Zhang , Huizhen Dong , Yaoyao Li , Fangcheng Bi , Xiaosan Huang , Tongxin Dou","doi":"10.1016/j.plantsci.2025.112495","DOIUrl":"10.1016/j.plantsci.2025.112495","url":null,"abstract":"<div><div>Banana, a globally cultivated fruit, faces significant constraints in distribution and sustainable production due to drought stress. This study investigated drought tolerance in <em>Cavendish bananas</em> using RNA-seq time-course analysis and molecular biology experiments. Plants were subjected to dehydration treatments, and physiological indicators such as electrolyte leakage, proline content, malonaldehyde, peroxidase activity, and hydrogen peroxide content were assessed. RNA-Seq and qRT-PCR were used to analyze transcriptional changes under drought. Weighted gene co-expression network (WGCNA) analysis identified thousands of differentially expressed genes (DEGs) at different time points, with a core set of 2660 DEGs consistently identified. KEGG enrichment analysis revealed <em>MaGME777</em>, a glycolysis/gluconeogenesis gene, as a potential drought resistance regulator. Virus-mediated gene silencing (VIGS) of <em>MaGME777</em> reduced drought tolerance in bananas. Yeast one-hybrid (Y1H) and luciferase reporter assays demonstrated that the transcription factor MabHLH770 directly binds and activates the <em>MaGME777</em> promoter. VIGS downregulation of <em>MabHLH770</em> also reduced drought tolerance. In conclusion, this study revealed that MabHLH770 is a positive regulator of drought stress, by targeting <em>MaGME777</em> promoter and activating their expression to enhance drought tolerance. These findings provide a foundation for developing drought-resistant banana cultivars through molecular breeding approaches.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112495"},"PeriodicalIF":4.2,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-17DOI: 10.1016/j.plantsci.2025.112513
Woo Joo Jung , Ji Hyeon Jeong , Jin Seok Yoon , Yong Weon Seo
Wheat (Triticum aestivum L.), a major global crop, is vulnerable to freezing stress, particularly during late spring frosts. Enhancing freezing tolerance through cold acclimation, primarily via the ICE-CBF-COR pathway, is crucial for improving wheat productivity. This study focuses on identifying genes regulated by the ICE-CBF pathway and those that function independently in response to freezing stress. TaICE41 and TaCBFⅣd-B9, two key genes associated with cold tolerance, were cloned and analyzed for their phylogenetic characteristics and subcellular localization. Transgenic Brachypodium distachyon overexpressing these genes demonstrated enhanced freezing tolerance, with increased survival rates and proline content, compared to wild-type plants. RNA-seq analysis revealed distinct gene expression profiles under cold stress, highlighting both shared and unique pathways regulated by ICE41 and CBF. Notably, the TaICE41-overexpressing lines exhibited upregulation of genes involved in phenylpropanoid biosynthesis and starch-sucrose metabolism, contributing to stress response. This study provides new insights into the ICE-CBF pathway and its role in cold tolerance, emphasizing the importance of understanding both ICE-CBF-regulated and independent cold-responsive genes for improving freezing tolerance in crops.
{"title":"Investigation of wheat cold response pathway regulated by TaICE41 and TaCBFⅣd-B9 through Brachypodium distachyon transformation","authors":"Woo Joo Jung , Ji Hyeon Jeong , Jin Seok Yoon , Yong Weon Seo","doi":"10.1016/j.plantsci.2025.112513","DOIUrl":"10.1016/j.plantsci.2025.112513","url":null,"abstract":"<div><div>Wheat (<em>Triticum aestivum</em> L.), a major global crop, is vulnerable to freezing stress, particularly during late spring frosts. Enhancing freezing tolerance through cold acclimation, primarily via the ICE-CBF-COR pathway, is crucial for improving wheat productivity. This study focuses on identifying genes regulated by the ICE-CBF pathway and those that function independently in response to freezing stress. <em>TaICE41</em> and <em>TaCBFⅣd-B9</em>, two key genes associated with cold tolerance, were cloned and analyzed for their phylogenetic characteristics and subcellular localization. Transgenic <em>Brachypodium distachyon</em> overexpressing these genes demonstrated enhanced freezing tolerance, with increased survival rates and proline content, compared to wild-type plants. RNA-seq analysis revealed distinct gene expression profiles under cold stress, highlighting both shared and unique pathways regulated by ICE41 and CBF. Notably, the <em>TaICE41</em>-overexpressing lines exhibited upregulation of genes involved in phenylpropanoid biosynthesis and starch-sucrose metabolism, contributing to stress response. This study provides new insights into the ICE-CBF pathway and its role in cold tolerance, emphasizing the importance of understanding both ICE-CBF-regulated and independent cold-responsive genes for improving freezing tolerance in crops.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112513"},"PeriodicalIF":4.2,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-15DOI: 10.1016/j.plantsci.2025.112516
Caixia Li , Juan Wang , Haiyan Lan , Qinghui Yu
The enzyme phosphoenolpyruvate carboxylase (PEPC) plays an important role in the photosynthetic metabolism of higher plants. Although the photosynthetic pathway involving PEPC has been clarified, further investigation is required to elucidate the effects of different light intensity treatments on plant photosynthetic and metabolism of PEPC. In this study, wild-type (WT) Arabidopsis was used as a control to investigate the effect of SaPEPC1 overexpression on the photosynthesis and metabolism of Arabidopsis. The results showed that intense light promoted and weak light inhibited the growth of Arabidopsis. Under different light intensity treatments, overexpression of SaPEPC1 led to increases in the photosynthetic rate (Pn) and photosynthetic enzyme activity (PEPC, Rubisco, PPDK, NADP-ME), a decrease in the intercellular CO2 concentration (Ci), and increases in sucrose accumulation, leaf length, leaf width, and shoot fresh weight. Transcriptomic data analysis revealed that the starch, sucrose, and glutathione metabolic pathways were significantly enriched in transgenic Arabidopsis under intense light. This was accompanied by the up-regulation of multiple differentially expressed genes related to starch and sucrose metabolism, including AtBAM5, AtSUS6, and AtTPS5; the expression of most genes related to glutathione metabolism was down-regulated. A targeted metabolomic data analysis of transgenic Arabidopsis yielded 56 metabolites, the majority of which were found to participate in the tricarboxylic acid (TCA) cycle, followed by glycolysis. The content of L-aspartate, fumaric acid, malic acid, oxaloacetate, citric acid, and succinic acid was higher in transgenic lines than in WT under intense light. In conclusion, the overexpression of SaPEPC1 in Arabidopsis resulted in an increase in the photosynthetic rate and promoted the TCA cycle, and these changes were more pronounced under intense light treatment.
{"title":"Comprehensive analyses of the metabolome and transcriptome reveal the photosynthetic effects in Arabidopsis thaliana of SaPEPC1 gene from desert plant with single-cell C4 photosynthetic pathway","authors":"Caixia Li , Juan Wang , Haiyan Lan , Qinghui Yu","doi":"10.1016/j.plantsci.2025.112516","DOIUrl":"10.1016/j.plantsci.2025.112516","url":null,"abstract":"<div><div>The enzyme phosphoenolpyruvate carboxylase (PEPC) plays an important role in the photosynthetic metabolism of higher plants. Although the photosynthetic pathway involving PEPC has been clarified, further investigation is required to elucidate the effects of different light intensity treatments on plant photosynthetic and metabolism of PEPC. In this study, wild-type (WT) <em>Arabidopsi</em>s was used as a control to investigate the effect of <em>SaPEPC1</em> overexpression on the photosynthesis and metabolism of <em>Arabidopsi</em>s. The results showed that intense light promoted and weak light inhibited the growth of <em>Arabidopsis</em>. Under different light intensity treatments, overexpression of <em>SaPEPC1</em> led to increases in the photosynthetic rate (<em>Pn</em>) and photosynthetic enzyme activity (PEPC, Rubisco, PPDK, NADP-ME), a decrease in the intercellular CO<sub>2</sub> concentration (<em>Ci</em>), and increases in sucrose accumulation, leaf length, leaf width, and shoot fresh weight. Transcriptomic data analysis revealed that the starch, sucrose, and glutathione metabolic pathways were significantly enriched in transgenic <em>Arabidopsis</em> under intense light. This was accompanied by the up-regulation of multiple differentially expressed genes related to starch and sucrose metabolism, including <em>AtBAM5</em>, <em>AtSUS6</em>, and <em>AtTPS5</em>; the expression of most genes related to glutathione metabolism was down-regulated. A targeted metabolomic data analysis of transgenic <em>Arabidopsis</em> yielded 56 metabolites, the majority of which were found to participate in the tricarboxylic acid (TCA) cycle, followed by glycolysis. The content of L-aspartate, fumaric acid, malic acid, oxaloacetate, citric acid, and succinic acid was higher in transgenic lines than in WT under intense light. In conclusion, the overexpression of <em>SaPEPC1</em> in <em>Arabidopsis</em> resulted in an increase in the photosynthetic rate and promoted the TCA cycle, and these changes were more pronounced under intense light treatment.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112516"},"PeriodicalIF":4.2,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-14DOI: 10.1016/j.plantsci.2025.112515
Guangcai Tang , Xin Cheng , Bingli Fan , Zhiqi Jia , Keke Liu , Shiwen Zhang
Polyamine oxidase (PAO) is a key enzyme in polyamine (PA) catabolism and plays a vital role during fruit ripening. However, regulatory mechanisms that control PAO expression during maturation remain unclear. This study identifies the transcription factor PpeERD15 through yeast one-hybrid (Y1H) screening with the PpePAO1 promoter. ERD15 (early response to dehydration 15), a member of the early response to dehydration protein family, is known for its role in abiotic stress responses, but its function in fruit ripening remains largely unexplored. Subcellular localization analysis demonstrated that PpeERD15 was localized in both the nucleus and cytoplasm. Y1H and LUC assays confirmed that PpeERD15 directly binds the PpePAO1 promoter. Transient silencing of PpEDR15 in peach fruit downregulated PpePAO1 expression, promoted PA accumulation, inhibited ethylene production, increased fruit firmness, and delayed fruit ripening. Conversely, overexpression of PpeEDR15 upregulated PpePAO1, decreased PA content, promoted ethylene production, reduced fruit firmness, and accelerated fruit ripening. The role of homologous gene of ERD15 was also validated in tomato. This study discovered that PpeEDR15 regulates fruit ripening by promoting PA catabolism via PpePAO1 expression.
{"title":"ERD15 promotes peach and tomato ripening by activating polyamine catabolism","authors":"Guangcai Tang , Xin Cheng , Bingli Fan , Zhiqi Jia , Keke Liu , Shiwen Zhang","doi":"10.1016/j.plantsci.2025.112515","DOIUrl":"10.1016/j.plantsci.2025.112515","url":null,"abstract":"<div><div>Polyamine oxidase (PAO) is a key enzyme in polyamine (PA) catabolism and plays a vital role during fruit ripening. However, regulatory mechanisms that control <em>PAO</em> expression during maturation remain unclear. This study identifies the transcription factor PpeERD15 through yeast one-hybrid (Y1H) screening with the <em>PpePAO1</em> promoter. ERD15 (early response to dehydration 15), a member of the early response to dehydration protein family, is known for its role in abiotic stress responses, but its function in fruit ripening remains largely unexplored. Subcellular localization analysis demonstrated that PpeERD15 was localized in both the nucleus and cytoplasm. Y1H and LUC assays confirmed that PpeERD15 directly binds the <em>PpePAO1</em> promoter. Transient silencing of <em>PpEDR15</em> in peach fruit downregulated <em>PpePAO1</em> expression, promoted PA accumulation, inhibited ethylene production, increased fruit firmness, and delayed fruit ripening. Conversely, overexpression of <em>PpeEDR15</em> upregulated <em>PpePAO1</em>, decreased PA content, promoted ethylene production, reduced fruit firmness, and accelerated fruit ripening. The role of homologous gene of <em>ERD15</em> was also validated in tomato. This study discovered that PpeEDR15 regulates fruit ripening by promoting PA catabolism via <em>PpePAO1</em> expression.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112515"},"PeriodicalIF":4.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-04-11DOI: 10.1016/j.plantsci.2025.112504
Sarika Kumari , Pravneet Kaur , Moksh Mahajan , Soumya Ranjan Nayak , Risheek Rahul Khanna , Md Tabish Rehman , Mohamed F. AlAjmi , M. Iqbal R. Khan
Arsenic (As) stress has been incessantly degrading crop productivity, and thereafter leading to the increasing grave conditions pertaining to the unsustainable food production. In plants, As stress has been considered as one of the serious phytotoxins persisting in the environment, endangering crop shelf life through competing with phosphorus availability. The withholding of As in the staple crop, wheat (Triticum aestivum), is the major concern. It has been advocated the significance of plant signaling molecules, γ-aminobutyric acid (GABA), in mediating plant health response to environmental stresses, but their impacts on As contamination in wheat plants from the perspective of growth and physiological tolerance still remain ambiguous at present. The present study investigated the significance of GABA supplementation in wheat plants on phosphorus and carbon metabolisms, adenosine triphosphatase (ATPase) activity, As accumulation, defense systems, and growth responses under As stress. In this study, GABA supplementation aided in the retention of phosphorus and carbon metabolites, sustained photosynthetic traits, and considerably modulated both chloroplastic and mitochondrial ATPase activity under As stress. Further, As-induced oxidative stress injuries were recovered through the activation of defense metabolites, and suppressed oxidative stress markers and As accumulation, which was found concomitant with the improved As tolerance index. Thus, this investigation offers insightful information that might be useful in future investigations to develop wheat tolerance to withstand under As-contaminated environments.
{"title":"γ-aminobutyric acid (GABA) supplementation modulates phosphorus retention, production of carbon metabolites and defense metabolism under arsenic toxicity in wheat","authors":"Sarika Kumari , Pravneet Kaur , Moksh Mahajan , Soumya Ranjan Nayak , Risheek Rahul Khanna , Md Tabish Rehman , Mohamed F. AlAjmi , M. Iqbal R. Khan","doi":"10.1016/j.plantsci.2025.112504","DOIUrl":"10.1016/j.plantsci.2025.112504","url":null,"abstract":"<div><div>Arsenic (As) stress has been incessantly degrading crop productivity, and thereafter leading to the increasing grave conditions pertaining to the unsustainable food production. In plants, As stress has been considered as one of the serious phytotoxins persisting in the environment, endangering crop shelf life through competing with phosphorus availability. The withholding of As in the staple crop, wheat (<em>Triticum aestivum</em>), is the major concern. It has been advocated the significance of plant signaling molecules, γ-aminobutyric acid (GABA), in mediating plant health response to environmental stresses, but their impacts on As contamination in wheat plants from the perspective of growth and physiological tolerance still remain ambiguous at present. The present study investigated the significance of GABA supplementation in wheat plants on phosphorus and carbon metabolisms, adenosine triphosphatase (ATPase) activity, As accumulation, defense systems, and growth responses under As stress. In this study, GABA supplementation aided in the retention of phosphorus and carbon metabolites, sustained photosynthetic traits, and considerably modulated both chloroplastic and mitochondrial ATPase activity under As stress. Further, As-induced oxidative stress injuries were recovered through the activation of defense metabolites, and suppressed oxidative stress markers and As accumulation, which was found concomitant with the improved As tolerance index. Thus, this investigation offers insightful information that might be useful in future investigations to develop wheat tolerance to withstand under As-contaminated environments.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":"356 ","pages":"Article 112504"},"PeriodicalIF":4.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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