427 Impact of late gestation slow-release nitrogen-enriched diets on energy metabolism in calf skeletal muscle: A proteomic and transcriptomic approach
Mateus P Gionbelli, Thais C Costa, Diana C Cediel-Devia, Karolina B Nascimento, Tathyane R S Gionbelli, Marcio S Duarte
{"title":"427 Impact of late gestation slow-release nitrogen-enriched diets on energy metabolism in calf skeletal muscle: A proteomic and transcriptomic approach","authors":"Mateus P Gionbelli, Thais C Costa, Diana C Cediel-Devia, Karolina B Nascimento, Tathyane R S Gionbelli, Marcio S Duarte","doi":"10.1093/jas/skae234.237","DOIUrl":null,"url":null,"abstract":"The current study aimed to determine the enriched biological processes, through proteomic and transcriptomic data, associated with maternal slow-release nitrogen diets received during late gestation and their effects on the skeletal muscle of offspring. From d 180 to d 268 of gestation, a total of 16 pregnant Brahman cows were divided into two groups: a control (CON, n = 12) low crude protein (CP) basal diet (6% of CP, ad libitum) plus mineral mixture (130 gּ cow-1ּ d-1), or the CON treatment supplemented with a slow-released N (SRN, n = 9) based protein concentrate supplement [40% CP, fed 2 g/kg of body weight (BW)/d in the morning) composed by corn, soybean meal, urea and a SRN source (Timafeed Boost, Roullier Group, Saint-Malo, France). Muscle biopsies were performed on d 45 of age in calves for RNA sequencing (RNA-seq) and proteomic (HPLC-MS/MS) analyses. The quality control of RNAseq raw data was assessed using FASTQc software, with low-quality reads trimmed by Trimmomatic. Reads were mapped against the Bos taurus reference genome using STAR. Differentially expressed (DE) genes and isoforms, significant at an adjusted P-value ≤ 0.05, were identified using the limma package and Cuffdiff tool, respectively, in the R environment. Proteomic data was processed in MaxQuant against the Bos taurus reference proteome, with statistical analysis performed using the MSqRob package in R. Network analyses identified significant biological processes (adjusted P-value ≤ 0.05) among differentially abundant proteins (DAPs). Notably, while the experimental treatment did not affect transcript abundance, protein-level differences were observed. Enriched biological processes in SRN group skeletal muscle of calves included acetyl-CoA biosynthesis from pyruvate, mitochondrial respiratory chain complex assembly, regulation of calcium ion transmembrane transport, and several others related to energy and nitrogen metabolism. Protein-protein interaction network analyses revealed key processes such as ATP and glucose metabolism, tricarboxylic acid cycle, and sarcomere organization. Overall, our findings underscore the beneficial impact of slow-release nitrogen-enriched diets during late gestation on energy metabolism in calf skeletal muscle.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"62 1","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skae234.237","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
The current study aimed to determine the enriched biological processes, through proteomic and transcriptomic data, associated with maternal slow-release nitrogen diets received during late gestation and their effects on the skeletal muscle of offspring. From d 180 to d 268 of gestation, a total of 16 pregnant Brahman cows were divided into two groups: a control (CON, n = 12) low crude protein (CP) basal diet (6% of CP, ad libitum) plus mineral mixture (130 gּ cow-1ּ d-1), or the CON treatment supplemented with a slow-released N (SRN, n = 9) based protein concentrate supplement [40% CP, fed 2 g/kg of body weight (BW)/d in the morning) composed by corn, soybean meal, urea and a SRN source (Timafeed Boost, Roullier Group, Saint-Malo, France). Muscle biopsies were performed on d 45 of age in calves for RNA sequencing (RNA-seq) and proteomic (HPLC-MS/MS) analyses. The quality control of RNAseq raw data was assessed using FASTQc software, with low-quality reads trimmed by Trimmomatic. Reads were mapped against the Bos taurus reference genome using STAR. Differentially expressed (DE) genes and isoforms, significant at an adjusted P-value ≤ 0.05, were identified using the limma package and Cuffdiff tool, respectively, in the R environment. Proteomic data was processed in MaxQuant against the Bos taurus reference proteome, with statistical analysis performed using the MSqRob package in R. Network analyses identified significant biological processes (adjusted P-value ≤ 0.05) among differentially abundant proteins (DAPs). Notably, while the experimental treatment did not affect transcript abundance, protein-level differences were observed. Enriched biological processes in SRN group skeletal muscle of calves included acetyl-CoA biosynthesis from pyruvate, mitochondrial respiratory chain complex assembly, regulation of calcium ion transmembrane transport, and several others related to energy and nitrogen metabolism. Protein-protein interaction network analyses revealed key processes such as ATP and glucose metabolism, tricarboxylic acid cycle, and sarcomere organization. Overall, our findings underscore the beneficial impact of slow-release nitrogen-enriched diets during late gestation on energy metabolism in calf skeletal muscle.
期刊介绍:
The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year.
Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.