A gain‐of‐function mutation at the C‐terminus of FT‐D1 promotes heading by interacting with 14‐3‐3A and FDL6 in wheat

IF 10.1 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Plant Biotechnology Journal Pub Date : 2024-09-14 DOI:10.1111/pbi.14474
Yuting Li, Hongchun Xiong, Huijun Guo, Yongdun Xie, Linshu Zhao, Jiayu Gu, Huiyuan Li, Shirong Zhao, Yuping Ding, Chunyun Zhou, Zhengwu Fang, Luxiang Liu
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Abstract

SummaryVernalization and photoperiod pathways converging at FT1 control the transition to flowering in wheat. Here, we identified a gain‐of‐function mutation in FT‐D1 that results in earlier heading date (HD), and shorter plant height and spike length in the gamma ray‐induced eh1 wheat mutant. Knockout of the wild‐type and overexpression of the mutated FT‐D1 indicate that both alleles are functional to affect HD and plant height. Protein interaction assays demonstrated that the frameshift mutation in FT‐D1eh1 exon 3 led to gain‐of‐function interactions with 14‐3‐3A and FDL6, thereby enabling the formation of florigen activation complex (FAC) and consequently activating a flowering‐related transcriptomic programme. This mutation did not affect FT‐D1eh1 interactions with TaNaKR5 or TaFTIP7, both of which could modulate HD, potentially via mediating FT‐D1 translocation to the shoot apical meristem. Furthermore, the ‘Segment B’ external loop is essential for FT‐D1 interaction with FDL6, while residue Y85 is required for interactions with TaNaKR5 and TaFTIP7. Finally, the flowering regulatory hub gene, ELF5, was identified as the FT‐D1 regulatory target. This study illustrates FT‐D1 function in determining wheat HD with a suite of interaction partners and provides genetic resources for tuning HD in elite wheat lines.
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小麦中 FT-D1 C 端的功能增益突变通过与 14-3-3A 和 FDL6 相互作用而促进头状花序的形成
摘要在 FT1 处汇聚的冬化和光周期途径控制着小麦向开花的过渡。在这里,我们发现了 FT-D1 的功能增益突变,该突变导致伽马射线诱导的 eh1 小麦突变体的打顶日期(HD)提前、株高和穗长缩短。敲除野生型和过表达突变的 FT-D1 表明,两个等位基因都具有影响 HD 和株高的功能。蛋白质相互作用测定表明,FT-D1eh1 外显子 3 的框架移位突变导致了与 14-3-3A 和 FDL6 的功能增益相互作用,从而使花粉激活复合物(FAC)得以形成,进而激活了与开花相关的转录组程序。这种突变并不影响 FT-D1eh1 与 TaNaKR5 或 TaFTIP7 的相互作用,这两种物质都可能通过介导 FT-D1 向嫩枝顶端分生组织的转位来调节 HD。此外,"B 段 "外环是 FT-D1 与 FDL6 相互作用的必要条件,而残基 Y85 则是与 TaNaKR5 和 TaFTIP7 相互作用的必要条件。最后,开花调控中枢基因 ELF5 被确定为 FT-D1 的调控靶标。这项研究说明了 FT-D1 在决定小麦 HD 与一系列相互作用伙伴中的功能,并为调整小麦精英品系的 HD 提供了遗传资源。
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来源期刊
Plant Biotechnology Journal
Plant Biotechnology Journal 生物-生物工程与应用微生物
CiteScore
20.50
自引率
2.90%
发文量
201
审稿时长
1 months
期刊介绍: Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.
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