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Ubiquitination-Mediated Degradation of SlVPS29 by the SlHSFA7-SlRNF185 Module Enhances Tomato Pollen Thermotolerance. SlHSFA7-SlRNF185模块介导的SlVPS29泛素化降解增强番茄花粉耐热性
IF 10.5 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-06 DOI: 10.1111/pbi.70583
Xiaolin Geng, Qinqin Yang, Hongwei Li, Xunwen Peng, Wenbo Zhang, Huihui Wang, Yushan Li, Fang Li, Quanquan Chen, Tao Lin

High temperature has posed significant challenges to global agriculture, markedly leading to reduced fertility and yield losses in tomato (Solanum lycopersicum). Therefore, thermotolerance-conferring genes and loci are needed to further improve cultivated tomatoes. Here we identified an E3 ubiquitin ligase SlRNF185, containing a C3HC4-type RING-HC domain, that confers tomato pollen thermotolerance. As a predominant ubiquitination member, SlRNF185 could degrade the SlVPS29 protein induced by heat stress to enhance thermotolerance. Mechanistically, we found that a heat shock transcription factor SlHSFA7 dramatically activates the expression of SlRNF185 under heat stress and acts as a positive regulator of tomato pollen thermotolerance. Collectively, our findings reveal that a SlHSFA7-SlRNF185 genetic module regulates ubiquitination-mediated degradation of SlVPS29 under heat stress, providing the strategy for breeding thermotolerance tomato varieties.

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引用次数: 0
An Elite Haplotype of Nitrogen-Use-Efficiency Gene LHT5 Enhances Salt Tolerance in Rice. 氮素利用效率基因LHT5单倍型提高水稻耐盐性
IF 10.5 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-06 DOI: 10.1111/pbi.70584
Saisai Wang, Xingzhou Jiang, Gaoming Chen, Wei Wu, Chen Xu, Mingyu Du, Shuji Xiang, Xinran Cheng, Yunlu Tian, Junjie Tan, Chunming Wang, Jianmin Wan

Amino acids serve as fundamental building blocks and signalling molecules in plants, orchestrating stress adaptation mechanisms against diverse biotic and abiotic environmental challenges. However, the mechanism by which plants alter their nutrient metabolism processes to coordinate nitrogen use efficiency (NUE) and salt tolerance remains elusive. Here, we identified a Lysine-Histidine-type transporter 5 (LHT5) gene through genome-wide association studies (GWAS) that enhances NUE via amino acid accumulation regulation. Further research showed that OsLHT5 also confers salt tolerance in rice by promoting proline biosynthesis through transcriptional upregulation of OsP5CS1 and OsP5CS2 genes, thereby increasing cellular proline levels for osmotic adjustment. Notably, we identified a functionally critical 30-bp deletion in the OsLHT5 coding region, designated as the elite haplotype LHT5HapA, which substantially enhances amino acid transport capacity and consequently improves both NUE and salt tolerance. Functional validation demonstrated that overexpression of LHT5HapA significantly increases amino acid content, nitrogen accumulation, grain yield and salt stress tolerance compared to the wildtype allele. This study establishes a novel molecular framework linking amino acid transport to the coordination of nutrient utilisation and stress tolerance, offering valuable genetic resources and breeding strategies for developing climate-resilient rice cultivars with enhanced productivity under both optimal and saline conditions.

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引用次数: 0
Tuning Rice Gene Expression via In Situ Promoter Truncations Using a Prime Editing Library. 利用引体编辑文库原位截断启动子调控水稻基因表达。
IF 10.5 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-06 DOI: 10.1111/pbi.70587
Yuying Li, Birong Xu, Xuanchang Gao, Ying Wang, Xiaoshuang Liu, Rongfang Xu, Juan Li, Pengcheng Wei, Ruiying Qin

Promoter engineering holds immense potential for fine-tuning gene expression and optimising agronomic traits, yet conventional genome-editing tools face limitations in precision, scalability, and risk mitigation. Here, we develop Prime Editing-mediated Promoter Engineering (PEPE), a DSB-free platform integrating bidirectional Protospacer adjacent motif (PAM) recognition (NGG/CCN) with combinatorial duo-pegRNA strategies to achieve tiled, overlapping deletions across entire plant promoters. Applying PEPE to the 1.8-kb rice D53 promoter, we generated a mutant library with stepwise deletions. Edited alleles showed stable inheritance, and dual-method validation confirmed the precision at junctions. Quantitative profiling revealed functional modularity: core-region deletions suppressed D53 expression by 70%-85%, while a distal deletion (D53-G9C10) paradoxically upregulated transcription 2.2-fold, uncovering a cryptic repressor element. Phenotypic variation corresponded with transcriptional changes, establishing a direct link between cis-regulatory diversity and agronomic traits. By circumventing DSBs and enabling kilobase-scale CRE manipulation, PEPE establishes a robust framework for decoding promoter logic and accelerating trait pyramiding in crops. This study advances plant genome editing by merging precision with scalability, offering transformative potential for breeding climate-resilient, high-yield cultivars.

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引用次数: 0
GmRPS5 Promoter‐Driven CRISPR/LbCas12a Efficiently Generates Soybean Sextuple Mutants GmRPS5启动子驱动CRISPR/LbCas12a高效生成大豆六重突变体
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-05 DOI: 10.1111/pbi.70588
Xiangchao Kong, Kexin Fan, Cuiping Xin, Minhui Lu, Yunlu Shi, Jie Jin, Qi‐Jun Chen
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引用次数: 0
OsWRKY53‐OsGT1 Module Regulates Rice Tiller Development and Is Involved in Fine‐Tuning Strigolactone Signaling OsWRKY53‐OsGT1模块调控水稻分蘖发育并参与孤孤内酯信号的微调
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-05 DOI: 10.1111/pbi.70578
Jiaqi Tang, Guilong Zhao, Jiangli Yang, Zhuo Chen, Zhipeng Hong, Xin Jin, Ziming Qiu, Zhenyu Wang, Xiufeng Li, Jijun Yan, Changhua Liu, Weiqiang Li, Jinfang Chu, Yuanhu Xuan, Xiaojie Tian, Qingyun Bu
Plant architecture, including plant height, tiller number, and leaf angle, is a critical determinant of rice yield. However, few genes have been identified that simultaneously regulate these traits and hold breeding value. We have previously shown that OsWRKY53 regulates the plant height and leaf angle via BR signalling. Here, we establish OsWRKY53 as a novel negative regulator of tillering in rice. The oswrky53 mutant exhibits a semi‐dwarf stature coupled with increased tiller number, representing a promising agronomic combination. Genetic and molecular analyses reveal that OsWRKY53 acts as a direct transcriptional activator of OsTB1 , thereby suppressing tiller formation. In addition, we found OsWRKY53 physically interacts with OsGT1, and their cooperative action synergistically enhances OsTB1 expression and suppresses tiller number. Intriguingly, the oswrky53 mutant exhibits reduced sensitivity to Strigolactone (SL) and increased SL contents. We further demonstrate that SL promotes degradation of OsWRKY53, and D53 interacts with and stabilises the OsWRKY53. Simultaneously, OsWRKY53 negatively regulates SL biosynthesis, enabling OsWRKY53 to function as a fine‐tuning regulator in the SL signalling pathway. Furthermore, OsGT1 exhibits subspecies‐specific regulation, with indica accessions carrying the OsGT1 581T allele showing significantly enhanced tillering capacity compared to japonica varieties. These findings collectively reveal the mechanism by which OsWRKY53 regulates the formation of tillers in rice, providing new genetic targets for semi‐dwarf and high‐tillering rice breeding.
{"title":"OsWRKY53‐OsGT1 Module Regulates Rice Tiller Development and Is Involved in Fine‐Tuning Strigolactone Signaling","authors":"Jiaqi Tang, Guilong Zhao, Jiangli Yang, Zhuo Chen, Zhipeng Hong, Xin Jin, Ziming Qiu, Zhenyu Wang, Xiufeng Li, Jijun Yan, Changhua Liu, Weiqiang Li, Jinfang Chu, Yuanhu Xuan, Xiaojie Tian, Qingyun Bu","doi":"10.1111/pbi.70578","DOIUrl":"https://doi.org/10.1111/pbi.70578","url":null,"abstract":"Plant architecture, including plant height, tiller number, and leaf angle, is a critical determinant of rice yield. However, few genes have been identified that simultaneously regulate these traits and hold breeding value. We have previously shown that OsWRKY53 regulates the plant height and leaf angle via BR signalling. Here, we establish OsWRKY53 as a novel negative regulator of tillering in rice. The <jats:italic>oswrky53</jats:italic> mutant exhibits a semi‐dwarf stature coupled with increased tiller number, representing a promising agronomic combination. Genetic and molecular analyses reveal that OsWRKY53 acts as a direct transcriptional activator of <jats:italic>OsTB1</jats:italic> , thereby suppressing tiller formation. In addition, we found OsWRKY53 physically interacts with OsGT1, and their cooperative action synergistically enhances <jats:italic>OsTB1</jats:italic> expression and suppresses tiller number. Intriguingly, the <jats:italic>oswrky53</jats:italic> mutant exhibits reduced sensitivity to Strigolactone (SL) and increased SL contents. We further demonstrate that SL promotes degradation of OsWRKY53, and D53 interacts with and stabilises the OsWRKY53. Simultaneously, OsWRKY53 negatively regulates SL biosynthesis, enabling OsWRKY53 to function as a fine‐tuning regulator in the SL signalling pathway. Furthermore, OsGT1 exhibits subspecies‐specific regulation, with <jats:italic>indica</jats:italic> accessions carrying the <jats:italic>OsGT1</jats:italic> <jats:sup> <jats:italic>581T</jats:italic> </jats:sup> allele showing significantly enhanced tillering capacity compared to <jats:italic>japonica</jats:italic> varieties. These findings collectively reveal the mechanism by which OsWRKY53 regulates the formation of tillers in rice, providing new genetic targets for semi‐dwarf and high‐tillering rice breeding.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"12 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146122467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Fusarium sacchari Glycoside Hydrolase 12 Protein FsEG1 Is a Major Virulence Factor During Sugarcane Infection and Confers Resistance to Pokkah Boeng Disease via the HIGS Strategy 镰刀菌糖苷水解酶12蛋白FsEG1是甘蔗感染过程中的一个主要毒力因子,并通过HIGS策略赋予甘蔗对Pokkah Boeng病的抗性
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1111/pbi.70577
Deng Wu, Tianshu Hong, Lulu Wang, Qianqian Ren, Shichao Wang, Yixue Bao, Muqing Zhang, Wei Yao, Qin Hu
Pokkah Boeng disease (PBD), caused by Fusarium sacchari , has severely impacted the yield and quality of sugarcane, resulting in significant economic losses. However, the molecular interaction mechanisms between F. sacchari and sugarcane remain poorly understood. In this study, we identified the GH12 family protein FsEG1, secreted by F. sacchari , as a critical virulence factor. Further analysis demonstrates that the hydrolase activity of FsEG1 is essential for the full virulence of F. sacchari . The enhanced immune responses and cell death induced by FsEG1 in N. benthamiana depend on the recognition of oligosaccharide elicitors derived from the degradation of host cell walls by FsEG1, which are detected by membrane‐localised receptors NbWAKs and NbCERK1, and this process also necessitates RAR1 and MAP3Kα to facilitate intracellular signal transduction. Consequently, FsEG1 activates DTI (DAMP‐triggered immunity) rather than the conventional PTI. The stable transgenic sugarcane plants carrying the FsEG1 RNAi hairpin construct displayed high levels of resistance to F. sacchari and decreased FsEG1 expression, production of specific FsEG1 siRNA in transgenic HIGS sugarcane plants was confirmed by stem‐loop qRT‐PCR; at the same time, the stable transgenic sugarcane plants with ectopic expression of FsEG1 also showed enhanced PBD resistance with activated expression of defence‐related genes. Overall, these findings establish a foundational basis for investigating the molecular mechanisms that govern the interactions between F. sacchari and sugarcane and offering valuable insights into enhancing sugarcane's resistance to PBD.
甘蔗赤霉病(Pokkah Boeng disease, PBD)是由甘蔗镰刀菌(Fusarium sacchari)引起的一种病害,严重影响甘蔗产量和品质,造成重大经济损失。然而,糖酵母菌与甘蔗之间的分子相互作用机制尚不清楚。在这项研究中,我们发现由F. sacchari分泌的GH12家族蛋白FsEG1是一个关键的毒力因子。进一步分析表明,FsEG1的水解酶活性对糖酵母菌的完全毒力至关重要。benthamiana中FsEG1诱导的免疫应答增强和细胞死亡依赖于FsEG1对宿主细胞壁降解产生的寡糖激发子的识别,这些诱导子被膜定位受体NbWAKs和NbCERK1检测到,并且这一过程还需要RAR1和MAP3Kα来促进细胞内信号转导。因此,FsEG1激活DTI (DAMP触发的免疫)而不是传统的PTI。携带FsEG1 RNAi发夹构建体的稳定转基因甘蔗植株对F. sacchari表现出高水平的抗性,FsEG1表达降低,通过茎环qRT - PCR证实了转基因甘蔗植株中FsEG1特异性siRNA的产生;同时,异位表达FsEG1的稳定转基因甘蔗植株也表现出抗性增强,防御相关基因的表达被激活。综上所述,这些研究结果为研究糖精酵母与甘蔗相互作用的分子机制奠定了基础,并为提高甘蔗对PBD的抗性提供了有价值的见解。
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引用次数: 0
Lily Transcription Factors LlPLATZ1 and LlMYB4 Orchestrate the Homeostasis of Heat Stress Responses via Antagonistic Regulation of LlHSF24 百合转录因子LlPLATZ1和LlMYB4通过拮抗LlHSF24调控热应激反应的稳态
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1111/pbi.70572
Xue Gong, Jun Xiang, Ziwei Liao, Tian Zhang, Liping Ding, Qianqian Fang, Nianjun Teng, Ze Wu
Heat stress significantly damages crop yield and quality. PLATZ (PLANT A/T‐RICH SEQUENCE‐AND ZINC‐BINDING PROTEIN) transcription factors play pivotal roles in plant growth, development, and environmental stress responses. While the functions of PLATZ members in response to drought and salt stress are well characterised, their roles in heat stress remain largely unexplored. Here, LlPLATZ1, a heat‐inducible member of the PLATZ family from lily ( Lilium longiflorum ), was identified. LlPLATZ1 was rapidly induced by high temperature, and its protein was localised to the nucleus, showing transcriptional repression activity. LlPLATZ1 bound to the promoter of a class B heat stress transcription factor gene, LlHSF24, to inhibit its expression. Stable overexpression of LlPLATZ1 in lily enhanced its thermotolerance, whereas silencing LlPLATZ1 had the opposite effect. Further analysis showed that LlHSF24 directly repressed the expression of heat‐protective genes LlHSP22.0 and LlHSP70 to weaken thermotolerance. In addition, LlPLATZ1 interacted with LlMYB4, a later heat‐inducible MYB transcription factor that bound to the LlHSF24 promoter to activate its expression. LlMYB4 limited the heat stress response by interacting with LlPLATZ1 to antagonise its DNA‐binding ability. In combination, these results indicate that the LlPLATZ1/LlMYB4‐LlHSF24 module may play a crucial role in maintaining a balanced heat stress response, enabling plants to adapt to complex environmental changes.
热胁迫严重损害作物产量和品质。PLATZ (PLANT A/T‐RICH SEQUENCE‐AND ZINC‐BINDING PROTEIN)转录因子在植物生长、发育和环境胁迫反应中起着关键作用。虽然PLATZ成员在干旱和盐胁迫下的功能已经很好地描述了,但它们在热胁迫中的作用仍然很大程度上未被探索。在这里,我们从百合(Lilium longiflorum)中鉴定出了一个热诱导型的PLATZ家族成员LlPLATZ1。LlPLATZ1被高温快速诱导,其蛋白定位于细胞核,表现出转录抑制活性。LlPLATZ1与B类热应激转录因子基因LlHSF24的启动子结合,抑制其表达。稳定过表达LlPLATZ1可以增强百合的耐热性,而沉默LlPLATZ1则相反。进一步分析发现,LlHSF24直接抑制热保护基因LlHSP22.0和LlHSP70的表达,从而减弱耐热性。此外,LlPLATZ1与LlMYB4相互作用,LlMYB4是一种后期热诱导的MYB转录因子,与LlHSF24启动子结合以激活其表达。LlMYB4通过与LlPLATZ1相互作用来对抗其DNA结合能力,从而限制了热应激反应。综上所述,这些结果表明LlPLATZ1/LlMYB4‐LlHSF24模块可能在维持平衡的热应激反应中发挥关键作用,使植物能够适应复杂的环境变化。
{"title":"Lily Transcription Factors LlPLATZ1 and LlMYB4 Orchestrate the Homeostasis of Heat Stress Responses via Antagonistic Regulation of LlHSF24","authors":"Xue Gong, Jun Xiang, Ziwei Liao, Tian Zhang, Liping Ding, Qianqian Fang, Nianjun Teng, Ze Wu","doi":"10.1111/pbi.70572","DOIUrl":"https://doi.org/10.1111/pbi.70572","url":null,"abstract":"Heat stress significantly damages crop yield and quality. PLATZ (PLANT A/T‐RICH SEQUENCE‐AND ZINC‐BINDING PROTEIN) transcription factors play pivotal roles in plant growth, development, and environmental stress responses. While the functions of PLATZ members in response to drought and salt stress are well characterised, their roles in heat stress remain largely unexplored. Here, LlPLATZ1, a heat‐inducible member of the PLATZ family from lily ( <jats:styled-content style=\"fixed-case\"> <jats:italic>Lilium longiflorum</jats:italic> </jats:styled-content> ), was identified. <jats:italic>LlPLATZ1</jats:italic> was rapidly induced by high temperature, and its protein was localised to the nucleus, showing transcriptional repression activity. LlPLATZ1 bound to the promoter of a class B heat stress transcription factor gene, <jats:italic>LlHSF24,</jats:italic> to inhibit its expression. Stable overexpression of <jats:italic>LlPLATZ1</jats:italic> in lily enhanced its thermotolerance, whereas silencing <jats:italic>LlPLATZ1</jats:italic> had the opposite effect. Further analysis showed that LlHSF24 directly repressed the expression of heat‐protective genes <jats:italic>LlHSP22.0</jats:italic> and <jats:italic>LlHSP70</jats:italic> to weaken thermotolerance. In addition, LlPLATZ1 interacted with LlMYB4, a later heat‐inducible MYB transcription factor that bound to the <jats:italic>LlHSF24</jats:italic> promoter to activate its expression. LlMYB4 limited the heat stress response by interacting with LlPLATZ1 to antagonise its DNA‐binding ability. In combination, these results indicate that the LlPLATZ1/LlMYB4‐LlHSF24 module may play a crucial role in maintaining a balanced heat stress response, enabling plants to adapt to complex environmental changes.","PeriodicalId":221,"journal":{"name":"Plant Biotechnology Journal","volume":"1 1","pages":""},"PeriodicalIF":13.8,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146115588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Specific Sinorhizobium Flagellin Suppresses Legume Nodulation Through Immune Activation 一种特异的中华根瘤菌鞭毛蛋白通过免疫激活抑制豆科植物结瘤
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1111/pbi.70576
Li Liu, Yaohua Li, Shuangcheng Ye, Chuanzheng Wei, Ziang Yan, Huanhui Yang, Hui Zhu, Gary Stacey, Yangrong Cao
Bacterial flagellin‐activated immunity plays a crucial role in shaping plant‐microbe interactions, leading to either parasitism, mutualism, or commensalism. In the legume‐rhizobium symbiosis, while it has been hypothesized that rhizobial infection involves avoidance of plant immunity following flagellin perception, direct evidence supporting this regulation remains unclear. Here, we conducted bioinformatic analyses of flagellin variations across the genus Sinorhizobium and identified a specific variant of the flagellin‐derived peptide, flg22 Sin ‐II (clade II flg22 from Sinorhizobium genus), which acts as an immunity elicitor during nodulation. Flg22 Sin ‐II, but not flg22 Sin ‐I or flg22 Sin ‐III, activates immune responses, including reactive oxygen species production, MPK phosphorylation, and immunity‐related gene expression in soybean, with Tyr‐7 being critical for the immune activation. Three different Sinorhizobium mutants knocking out the flagellin that produces flg22 Sin ‐II enhanced nodulation across three diverse legume species, highlighting how beneficial microbes modulate host immunity to optimize symbiotic interactions. Soybean gmfls2a gmfls2b double mutant lacking both flagellin receptors, GmFLS2a and GmFLS2b, exhibited an increased nodule number following S. fredii HH103 inoculation and showed reduced expression of immune‐related genes in nodules. Rather than complete immune evasion, the retention of an immune‐activating flagellin epitope by Sinorhizobium likely represents a sophisticated coevolutionary strategy to actively modulate host responses, ensuring symbiotic homeostasis and preventing detrimental over‐colonisation.
细菌鞭毛蛋白激活的免疫在形成植物与微生物的相互作用中起着至关重要的作用,导致寄生、互惠或共生。在豆科植物-根瘤菌共生中,虽然有人假设根瘤菌感染涉及在鞭毛蛋白感知后避免植物免疫,但支持这一调节的直接证据尚不清楚。在这里,我们对整个Sinorhizobium属的鞭毛蛋白变异进行了生物信息学分析,并鉴定了鞭毛蛋白衍生肽flg22 Sin - II(来自Sinorhizobium属的分支II flg22)的特定变体,该变体在结瘤过程中起免疫激发剂的作用。Flg22 Sin‐II,而不是Flg22 Sin‐I或Flg22 Sin‐III,可以激活免疫反应,包括活性氧产生、MPK磷酸化和大豆免疫相关基因表达,其中Tyr‐7对免疫激活至关重要。三种不同的Sinorhizobium突变体敲除产生flg22 Sin - II的鞭毛蛋白,增强了三种不同豆科植物的结瘤,突出了有益微生物如何调节宿主免疫以优化共生相互作用。缺乏鞭毛蛋白受体的大豆gmfls2a和gmfls2b双突变体在接种fredii HH103后显示出结节数量增加,且结节中免疫相关基因的表达减少。与完全的免疫逃避相比,中国根瘤菌对免疫激活鞭毛蛋白表位的保留可能代表了一种复杂的协同进化策略,以积极调节宿主反应,确保共生稳态并防止有害的过定殖。
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引用次数: 0
A Highly Conserved SNARE ‐Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking 一种高度保守的SNARE相关蛋白通过调节囊泡运输增强植物免疫力
IF 13.8 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-04 DOI: 10.1111/pbi.70573
Tracy E. Hawk, Peitong Li, Hafiz Muhammad Khalid Abbas, Mst Shamira Sultana, Sarbottam Piya, Nicole Coffey, Cengizhan Öztürk, Sobhan Bahrami Zadegan, Susan Thomas Laird, Mazen Alazem, Samantha P. Nuzzi, J. Hollis Rice, Chris Wyman, Tessa Burch‐Smith, Vince Pantalone, Tarek Hewezi
The plant endomembrane system and vesicle trafficking are central to plant immunity, mediating the targeted delivery and recycling of defence molecules during pathogen attack. Here, we investigated the functional role of soybean Vacuole Membrane Protein 1 (GmVMP1) in mediating resistance against soybean cyst nematode (SCN, Heterodera glycines ). GmVMP1 is a SNARE‐associated protein whose expression was specifically and transiently upregulated in SCN‐resistant soybean roots. Overexpression of GmVMP1 conferred near‐complete resistance to SCN. Field‐grown transgenic lines showed no developmental penalties and displayed modest gains in seed weight and protein content. GmVMP1 interacted with four key vesicle trafficking proteins, and silencing these interactors compromised GmVMP1‐mediated resistance. Live‐cell imaging revealed that GmVMP1 enhances endocytic vesicle formation and accelerates internalisation dynamics, pointing to a role in membrane trafficking during defence activation. Together, these results establish GmVMP1 as a novel SCN resistance gene that modulates vesicle trafficking to support early defence, with promising agronomic traits for soybean improvement.
植物膜系统和囊泡运输是植物免疫的核心,在病原体攻击期间介导防御分子的靶向递送和再循环。在此,我们研究了大豆液泡膜蛋白1 (GmVMP1)在介导对大豆囊肿线虫(SCN, Heterodera glycine)抗性中的功能作用。GmVMP1是一种SNARE相关蛋白,其表达在抗SCN的大豆根系中特异性且短暂上调。GmVMP1的过表达赋予了对SCN近乎完全的抗性。田间种植的转基因品系没有表现出发育不良,种子重量和蛋白质含量略有增加。GmVMP1与四个关键的囊泡运输蛋白相互作用,沉默这些相互作用蛋白会损害GmVMP1介导的耐药性。活细胞成像显示,GmVMP1增强了内吞囊泡的形成,加速了内化动力学,指出了防御激活过程中膜运输的作用。总之,这些结果表明GmVMP1是一种新的SCN抗性基因,它调节囊泡运输以支持早期防御,具有改善大豆的有希望的农艺性状。
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引用次数: 0
A Super-Pangenome for Cultivated Citrus Reveals Evolutive Features During the Allopatric Phase of Their Reticulate Evolution. 栽培柑橘的超级泛基因组揭示了其网状进化的异域阶段的进化特征。
IF 10.5 1区 生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2026-02-03 DOI: 10.1111/pbi.70553
Gaetan Droc, Delphine Giraud, Caroline Belser, Karine Labadie, Simone Duprat, Corinne Cruaud, Benjamin Istace, Fredson Dos Santos Menezes, Edson Mario de Andrade Silva, Franck Curk, Gilles Costantino, Alexandre Soriano, Pierre Mournet, Alexis Dereeper, Maëva Miranda, Elodie Marchi, Sylvain Santoni, Raner José Santana Silva, Stéphanie Sidibe-Bocs, François Luro, Nathalie Choisne, Florian Maumus, Barbara Hufnagel, Fabienne Micheli, Patrick Wincker, Jean-Marc Aury, Arnaud Lemainque, Patrick Ollitrault

The main genetic diversity observed in cultivated citrus results from a reticulate evolution involving four ancestral taxa whose radiation occurred in allopatry. In such context, GWAS analysis, genome diversity and transcriptomic studies will be significantly enhanced through pangenome approaches. We report the implementation of a super-pangenome for cultivated citrus, established with de novo assemblies of C. medica, C. reticulata and C. micrantha, released for the first time alongside a published chromosome-scale assembly of C. maxima. Repetitive element annotation revealed that half of each genome consisted of transposable elements or DNA-satellites. The new genome assemblies display strong synteny and collinearity, while discrepancies are observed with the C. maxima assembly. Resequencing information from 55 accessions helped to explore the intra- and interspecific diversity of the ancestral taxa and their relationships with horticultural groups. Diagnostic SNPs of the ancestral taxa revealed interspecific introgressions in several representative accessions of C. reticulata, C. maxima and C. medica as well as insights into the origin and phylogenomic structures of horticultural groups. PAV analysis revealed a gene whose absence or presence was specific to one of the ancestral taxa. Diagnostic PAV analysis uncovered a large chloroplastic introgression in C. medica chromosome 4. The analysis of the functional enrichment and species-specific adaptations in the citrus super-pangenome revealed distinct functional specialisations. This highlights the evolutionary paths that have shaped species, contributing to the diversity in the citrus super-pangenome while maintaining a shared foundation of essential biological processes. We established a Genome Hub, offering a platform for continuous genomic research.

栽培柑橘的主要遗传多样性来自于一个网状进化,涉及四个祖先类群,其辐射发生在异域。在这种背景下,GWAS分析、基因组多样性和转录组学研究将通过泛基因组方法得到显著加强。我们报道了对栽培柑橘的超级泛基因组的实现,该基因组是用C. medica、C. reticulata和C. micrantha重新组装而成的,这是首次与已发表的C. maxima染色体规模组装一起发布。重复元素注释显示,每个基因组有一半由转座元件或dna卫星组成。新的基因组组合显示出较强的共线性,但与C. maxima组合存在差异。55份材料的重排序信息有助于探索其祖先分类群的种内和种间多样性及其与园艺类群的关系。该祖先分类群的诊断性snp揭示了几个代表性品种的种间渗透,并对园艺类群的起源和系统基因组结构有了新的认识。PAV分析揭示了一个基因的缺失或存在是一个祖先分类群所特有的。诊断性PAV分析发现,紫花草第4染色体存在较大的叶绿体渗入。柑橘超级泛基因组的功能富集和物种特异性适应分析揭示了不同的功能特化。这突出了形成物种的进化路径,促进了柑橘超级泛基因组的多样性,同时保持了基本生物过程的共享基础。我们建立了一个基因组中心,为持续的基因组研究提供一个平台。
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引用次数: 0
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Plant Biotechnology Journal
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