Dairy liquid waste as substrate to obtain biotechnological chondroitin: A circular economy approach

IF 3.5 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food and Bioproducts Processing Pub Date : 2024-09-13 DOI:10.1016/j.fbp.2024.09.007
{"title":"Dairy liquid waste as substrate to obtain biotechnological chondroitin: A circular economy approach","authors":"","doi":"10.1016/j.fbp.2024.09.007","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial polysaccharides have been gaining growing interest often as alternative to animal derived products or as sources of novel features for biotechnological applications. Process production costs, however, are still high. A possible solution to that exploits agri-food and dairy industrial byproducts as fermentation substrates. This approach also reduces the need for cost-intensive disposal treatments for these waste sources and supports green and circular economy policies. Therefore, as for other microbial glucuronic acid-based biopolymers (e.g. hyaluronic acid, alginate), in this perspective, wild type and engineered <em>E. coli</em> K4 were used in this work as cell factories to produce K4 capsular polysaccharide (CPS) from renewable sources. The backbone of the K4 CPS, chondroitin, is the precursor of chondroitin sulfate (CS), a glycosaminoglycan found in animal tissues that is extensively used for curing osteoarthritis and studied for several other emerging biomedical applications. Interestingly, also chondroitin showed promising bioactivity <em>in vitro</em> and <em>in vivo</em>. Due to its high availability from local companies, second cheese whey (SCW), a worldwide copious and polluting liquid waste, was used as fermentation substrate in this work. Results showed that SCW fully supports growth of wild type and recombinant <em>E. coli</em> K4 strains, and demonstrate, for the first time up to date, the production of K4 CPS from liquid waste as proof of principle. Batch processes in 3 L fermenters indicated a 100 % improvement of the polysaccharide yield and allowed the production of 1.1±0.1 g/L of product from the recombinant strain with very low accumulation of acetic acid, demonstrating that SCW by itself fully supports polysaccharide production.</p></div>","PeriodicalId":12134,"journal":{"name":"Food and Bioproducts Processing","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food and Bioproducts Processing","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0960308524001809","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Microbial polysaccharides have been gaining growing interest often as alternative to animal derived products or as sources of novel features for biotechnological applications. Process production costs, however, are still high. A possible solution to that exploits agri-food and dairy industrial byproducts as fermentation substrates. This approach also reduces the need for cost-intensive disposal treatments for these waste sources and supports green and circular economy policies. Therefore, as for other microbial glucuronic acid-based biopolymers (e.g. hyaluronic acid, alginate), in this perspective, wild type and engineered E. coli K4 were used in this work as cell factories to produce K4 capsular polysaccharide (CPS) from renewable sources. The backbone of the K4 CPS, chondroitin, is the precursor of chondroitin sulfate (CS), a glycosaminoglycan found in animal tissues that is extensively used for curing osteoarthritis and studied for several other emerging biomedical applications. Interestingly, also chondroitin showed promising bioactivity in vitro and in vivo. Due to its high availability from local companies, second cheese whey (SCW), a worldwide copious and polluting liquid waste, was used as fermentation substrate in this work. Results showed that SCW fully supports growth of wild type and recombinant E. coli K4 strains, and demonstrate, for the first time up to date, the production of K4 CPS from liquid waste as proof of principle. Batch processes in 3 L fermenters indicated a 100 % improvement of the polysaccharide yield and allowed the production of 1.1±0.1 g/L of product from the recombinant strain with very low accumulation of acetic acid, demonstrating that SCW by itself fully supports polysaccharide production.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
以乳制品废液为基质获取生物软骨素:循环经济方法
微生物多糖作为动物衍生产品的替代品或生物技术应用的新特性来源,正日益受到人们的关注。然而,加工生产成本仍然很高。一种可行的解决方案是利用农业食品和乳制品工业副产品作为发酵底物。这种方法还能减少对这些废物进行成本高昂的处理,支持绿色和循环经济政策。因此,与其他以微生物葡萄糖醛酸为基础的生物聚合物(如透明质酸、海藻酸)一样,在这项研究中,野生型和工程大肠杆菌 K4 被用作细胞工厂,利用可再生来源生产 K4 胶囊多糖(CPS)。K4 CPS 的骨架--软骨素是硫酸软骨素(CS)的前体,CS 是一种存在于动物组织中的糖胺聚糖,被广泛用于治疗骨关节炎,并被研究用于其他一些新兴的生物医学应用。有趣的是,软骨素在体外和体内也显示出良好的生物活性。二次乳清(SCW)是一种全球性的大量污染液体废物,由于可从当地公司大量获得,因此在这项工作中被用作发酵基质。结果表明,SCW 完全支持野生型和重组大肠杆菌 K4 菌株的生长,并首次证明了利用液体废物生产 K4 CPS 的原理。在 3 升发酵罐中进行的批处理表明,多糖产量提高了 100%,重组菌株可生产出 1.1±0.1 克/升的产品,乙酸累积量极低,这表明 SCW 本身完全支持多糖生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
期刊最新文献
Synthesis, characterization, and evaluation of antibacterial properties of silver/ carboxymethyl cellulose/ bacterial cellulose/ Clitoria ternatea extract aerogel composites Freeze, spray, and vacuum dried Camelina sativa protein powders and their physicochemical and functional properties Exploring solution composition and vacuum pulse effects in the osmotic processing of sole (Paralichthys sp.) fillets Nanoencapsulation of Spirulina sp. LEB 18 microalgae biomass using electrospray technique and application in chocolate milk Construction and characteristics of EGCG-porcine serum albumin pickering emulsion: Based on noncovalent interactions mechanism
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1