Assessment of the potential susceptibility of planktonic cells and bacterial biofilms by diosgenin

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Bioscience Pub Date : 2024-09-13 DOI:10.1016/j.fbio.2024.105106
Maria do Socorro Costa , Ana Raquel Pereira da Silva , Nara Juliana Santos Araújo , Cícera Laura Roque Paulo , Gabriel Gonçalves Alencar , Maria Anesia Sousa de Alencar , Talysson Felismino Moura , Clara Mariana Gonçalves Lima , Thiago Sampaio de Freitas , José Maria Barbosa Filho , Jacqueline Cosmo Andrade-Pinheiro , Henrique Douglas Melo Coutinho
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Abstract

Bacterial biofilms are formed by a complex community of microorganisms, held together by a matrix that provides structural support and stability to microbial communities, making infections associated with biofilms more difficult to treat. In the face of growing research in the health area in search of antibiotics with action against biofilms, natural products present an effective alternative strategy. In this context, the aim of this study was to evaluate bacterial biofilm formation, assess the susceptibility of planktonic cells to diosgenin, and examine its efficacy on inhibiting and treating biofilms. The broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC). Six bacterial strains were tested: Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 9027, Pseudomonas aeruginosa 24, Enterococcus faecalis INCQS 0017, Enterococcus faecalis INCQS 00018, and Enterococcus faecalis INCQS 654. The crystal violet method was employed to evaluate the potential of diosgenin in biofilm inhibition and eradication. Chlorhexidine gluconate served as a reference standard for the antibiofilm assays, along with the antibiotics norfloxacin, ampicillin, and gentamicin. MIC determination revealed that diosgenin exhibited intrinsic antimicrobial activity, with an MIC of 406 μg/mL against S. aureus ATCC 25923. The antibiofilm activity showed significant inhibition for E. faecalis 17 and moderate inhibition against E. faecalis 654 and S. aureus 25923. Furthermore, diosgenin effectively eradicated preformed biofilms of S. aureus 25923 and P. aeruginosa 9027, indicating its ability to interfere with biofilm biomass. These results suggest that diosgenin not only inhibits bacterial biofilm formation but also eradicates established biofilms, highlighting it as a promising candidate for the development of products aimed at combating bacterial biofilms.

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评估浮游细胞和细菌生物膜对双歧因子的潜在敏感性
细菌生物膜是由复杂的微生物群落形成的,由基质固定在一起,为微生物群落提供结构支撑和稳定性,这使得与生物膜相关的感染更加难以治疗。面对健康领域越来越多的研究在寻找对生物膜有作用的抗生素,天然产品提供了一种有效的替代策略。在此背景下,本研究旨在评估细菌生物膜的形成,评估浮游细胞对地奥司宁的敏感性,并研究其抑制和治疗生物膜的功效。采用肉汤微稀释法测定最低抑菌浓度(MIC)。测试了六种细菌菌株:金黄色葡萄球菌 ATCC 25923、铜绿假单胞菌 ATCC 9027、铜绿假单胞菌 24、粪肠球菌 INCQS 0017、粪肠球菌 INCQS 00018 和粪肠球菌 INCQS 654。采用结晶紫法评估了薯蓣皂苷抑制和消除生物膜的潜力。葡萄糖酸氯己定与抗生素诺氟沙星、氨苄西林和庆大霉素一起作为抗生物膜测定的参考标准。MIC 测定显示,薯蓣皂苷具有内在抗菌活性,对金黄色葡萄球菌 ATCC 25923 的 MIC 为 406 μg/mL。抗生物膜活性显示,对粪肠球菌 17 有显著抑制作用,对粪肠球菌 654 和金黄色葡萄球菌 25923 有中等抑制作用。此外,薯蓣皂苷还能有效消除金黄色葡萄球菌 25923 和绿脓杆菌 9027 预先形成的生物膜,这表明薯蓣皂苷具有干扰生物膜生物量的能力。这些结果表明,薯蓣皂苷不仅能抑制细菌生物膜的形成,还能根除已形成的生物膜,因此它是一种很有前途的候选物质,可用于开发旨在对付细菌生物膜的产品。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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