Analysis of randomly mutated AlSRKb genes reveals that most loss-of-function mutations cause defects in plasma membrane localization

IF 8.3 1区 生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-09-15 DOI:10.1111/nph.20111
Masaya Yamamoto, Shotaro Ohtake, Akihisa Shinozawa, Matsuyuki Shirota, Yuki Mitsui, Hiroyasu Kitashiba
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对随机突变的 AlSRKb 基因进行分析后发现,大多数功能缺失突变会导致质膜定位缺陷
位于 AlSRKb 分子内部的氨基酸残基在不同 S 单倍型中高度保守,这些氨基酸残基的突变导致氨基酸特性发生显著变化,而这些变化与 SI 缺陷有关。我们的分析表明,在导致 SI 缺陷的突变中,AlSRKb 生物合成异常比导致自我识别活性丧失的突变更常见。此外,我们还利用随机森林(RandomForest)和极端梯度提升(Extreme Gradient Boosting)方法对 164 个突变进行了初步预测,从而得出了关于连作植物转化体 SI 表型的结论。
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来源期刊
New Phytologist
New Phytologist 生物-植物科学
自引率
5.30%
发文量
728
期刊介绍: New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.
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