The MYB transcriptional factor BrMYB108 regulates Auxin-mediated delayed leaf senescence in postharvest Pak Choi

IF 6.4 1区农林科学 Q1 AGRONOMY Postharvest Biology and Technology Pub Date : 2024-09-18 DOI:10.1016/j.postharvbio.2024.113181

Xuesong Liu , Xiaoyang Xu , Yujun Zhang , Yinghao Xu , Xiaofei Chen , Wen Huang , Pengxia Li

{"title":"The MYB transcriptional factor BrMYB108 regulates Auxin-mediated delayed leaf senescence in postharvest Pak Choi","authors":"Xuesong Liu , Xiaoyang Xu , Yujun Zhang , Yinghao Xu , Xiaofei Chen , Wen Huang , Pengxia Li","doi":"10.1016/j.postharvbio.2024.113181","DOIUrl":null,"url":null,"abstract":"<div>Auxin is the earliest discovered class of endogenous plant hormones, widely distributed in higher plants, and plays a key role in regulating plant growth and development. However, the molecular mechanism by which auxin affects postharvest leaf senescence in pak choi (Brassica rapa subsp. chinensis) remains unclear. In this study, we found that exogenous application of 2 mg L−1 of the auxin analog indoleacetic acid (IAA) could delay postharvest leaf senescence in pak choi. Importantly, we isolated an R2R3-MYB transcription factor (TF), BrMYB108, which was highly expressed in senescent leaves and whoes expression was suppressed by IAA treatment. Molecular evidence confirmed that BrMYB108 can bind to the promoter region of the chlorophyll metabolism gene BrSGR2 to activate its expression. Heterologous expression of BrMYB108 in Arabidopsis accelerated leaf senescence, while silencing BrMYB108 in pak choi using virus induced gene silencing (VIGS) delayed leaf senescence. In addition, we found that BrMYB108 is a direct target gene of the auxin response factor 17 (ARF17) homolog gene, BrARF17, in Arabidopsis. Our study reveals a model in which BrARF17 regulates postharvest leaf senescence in pak choi by targeting the expression of BrMYB108. These molecular insights into the auxin-mediated regulation of postharvest leaf senescence in pak choi have positive implications for manipulating BrMYB108 to improve the postharvest shelf life of vegetables.</div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113181"},"PeriodicalIF":6.4000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004265/pdfft?md5=22df9271c69ec5683856b342605f0e4b&pid=1-s2.0-S0925521424004265-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424004265","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Auxin is the earliest discovered class of endogenous plant hormones, widely distributed in higher plants, and plays a key role in regulating plant growth and development. However, the molecular mechanism by which auxin affects postharvest leaf senescence in pak choi (Brassica rapa subsp. chinensis) remains unclear. In this study, we found that exogenous application of 2 mg L⁻¹ of the auxin analog indoleacetic acid (IAA) could delay postharvest leaf senescence in pak choi. Importantly, we isolated an R2R3-MYB transcription factor (TF), BrMYB108, which was highly expressed in senescent leaves and whoes expression was suppressed by IAA treatment. Molecular evidence confirmed that BrMYB108 can bind to the promoter region of the chlorophyll metabolism gene BrSGR2 to activate its expression. Heterologous expression of BrMYB108 in Arabidopsis accelerated leaf senescence, while silencing BrMYB108 in pak choi using virus induced gene silencing (VIGS) delayed leaf senescence. In addition, we found that BrMYB108 is a direct target gene of the auxin response factor 17 (ARF17) homolog gene, BrARF17, in Arabidopsis. Our study reveals a model in which BrARF17 regulates postharvest leaf senescence in pak choi by targeting the expression of BrMYB108. These molecular insights into the auxin-mediated regulation of postharvest leaf senescence in pak choi have positive implications for manipulating BrMYB108 to improve the postharvest shelf life of vegetables.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

MYB转录因子BrMYB108调控采后白菜中由叶绿素介导的叶片延迟衰老

辅酶是最早发现的一类内源植物激素，广泛分布于高等植物中，在调节植物生长和发育中起着关键作用。然而，辅助素影响白菜（Brassica rapa subsp.chinensis）采后叶片衰老的分子机制仍不清楚。在这项研究中，我们发现外源施用 2 mg L-1 的辅助素类似物吲哚乙酸（IAA）可以延迟白菜采后叶片的衰老。重要的是，我们分离出了一种R2R3-MYB转录因子（TF）--BrMYB108，它在衰老叶片中高表达，IAA处理抑制了它的表达。分子证据证实，BrMYB108能与叶绿素代谢基因BrSGR2的启动子区域结合，激活其表达。在拟南芥中异源表达BrMYB108可加速叶片衰老，而利用病毒诱导基因沉默（VIGS）在白菜中沉默BrMYB108可延缓叶片衰老。此外，我们还发现 BrMYB108 是拟南芥中辅助因子反应因子 17（ARF17）同源基因 BrARF17 的直接靶基因。我们的研究揭示了一个模型，在该模型中，BrARF17 通过靶向表达 BrMYB108 来调控白菜采后叶片的衰老。这些关于辅助素介导的茄子采后叶片衰老调控的分子见解，对于操纵 BrMYB108 以改善蔬菜采后货架期具有积极意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.