{"title":"The MYB transcriptional factor BrMYB108 regulates Auxin-mediated delayed leaf senescence in postharvest Pak Choi","authors":"","doi":"10.1016/j.postharvbio.2024.113181","DOIUrl":null,"url":null,"abstract":"<div><p>Auxin is the earliest discovered class of endogenous plant hormones, widely distributed in higher plants, and plays a key role in regulating plant growth and development. However, the molecular mechanism by which auxin affects postharvest leaf senescence in pak choi (<em>Brassica rapa</em> subsp. <em>chinensis</em>) remains unclear. In this study, we found that exogenous application of 2 mg L<sup>−1</sup> of the auxin analog indoleacetic acid (IAA) could delay postharvest leaf senescence in pak choi. Importantly, we isolated an R2R3-MYB transcription factor (TF), <em>BrMYB108</em>, which was highly expressed in senescent leaves and whoes expression was suppressed by IAA treatment. Molecular evidence confirmed that BrMYB108 can bind to the promoter region of the chlorophyll metabolism gene <em>BrSGR2</em> to activate its expression. Heterologous expression of <em>BrMYB108</em> in Arabidopsis accelerated leaf senescence, while silencing <em>BrMYB108</em> in pak choi using virus induced gene silencing (VIGS) delayed leaf senescence. In addition, we found that <em>BrMYB108</em> is a direct target gene of the auxin response factor 17 (ARF17) homolog gene, <em>BrARF17</em>, in Arabidopsis. Our study reveals a model in which BrARF17 regulates postharvest leaf senescence in pak choi by targeting the expression of <em>BrMYB108</em>. These molecular insights into the auxin-mediated regulation of postharvest leaf senescence in pak choi have positive implications for manipulating <em>BrMYB108</em> to improve the postharvest shelf life of vegetables.</p></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":null,"pages":null},"PeriodicalIF":6.4000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0925521424004265/pdfft?md5=22df9271c69ec5683856b342605f0e4b&pid=1-s2.0-S0925521424004265-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424004265","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
Auxin is the earliest discovered class of endogenous plant hormones, widely distributed in higher plants, and plays a key role in regulating plant growth and development. However, the molecular mechanism by which auxin affects postharvest leaf senescence in pak choi (Brassica rapa subsp. chinensis) remains unclear. In this study, we found that exogenous application of 2 mg L−1 of the auxin analog indoleacetic acid (IAA) could delay postharvest leaf senescence in pak choi. Importantly, we isolated an R2R3-MYB transcription factor (TF), BrMYB108, which was highly expressed in senescent leaves and whoes expression was suppressed by IAA treatment. Molecular evidence confirmed that BrMYB108 can bind to the promoter region of the chlorophyll metabolism gene BrSGR2 to activate its expression. Heterologous expression of BrMYB108 in Arabidopsis accelerated leaf senescence, while silencing BrMYB108 in pak choi using virus induced gene silencing (VIGS) delayed leaf senescence. In addition, we found that BrMYB108 is a direct target gene of the auxin response factor 17 (ARF17) homolog gene, BrARF17, in Arabidopsis. Our study reveals a model in which BrARF17 regulates postharvest leaf senescence in pak choi by targeting the expression of BrMYB108. These molecular insights into the auxin-mediated regulation of postharvest leaf senescence in pak choi have positive implications for manipulating BrMYB108 to improve the postharvest shelf life of vegetables.
期刊介绍:
The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages.
Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing.
Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.