Longfang Yao , Li Zhang , Liwen Chen , Yiyan Fei , Simone Lamon , Min Gu , Lan Mi , Jing Wang , Jiong Ma
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引用次数: 0
Abstract
Expansion Microscopy (ExM) is a widely used super-resolution technique that enables imaging of structures beyond the diffraction limit of light. However, ExM suffers from weak labeling signals and expansion distortions, limiting its applicability. Here, we present an innovative approach called Tetrahedral DNA nanostructure Expansion Microscopy (TDN-ExM), addressing these limitations by using tetrahedral DNA nanostructures (TDNs) for fluorescence labeling. Our approach demonstrates a 3- to 10-fold signal amplification due to the multivertex nature of TDNs, allowing the modification of multiple dyes. Previous studies have confirmed minimal distortion on a large scale, and our strategy can reduce the distortion at the ultrastructural level in samples because it does not rely on anchoring agents and is not affected by digestion. This results in a brighter fluorescence, better uniformity, and compatibility with different labeling strategies and optical super-resolution technologies. We validated the utility of TDN-ExM by imaging various biological structures with improved resolutions and signal-to-noise ratios.
期刊介绍:
The Journal of Photochemistry and Photobiology B: Biology provides a forum for the publication of papers relating to the various aspects of photobiology, as well as a means for communication in this multidisciplinary field.
The scope includes:
- Bioluminescence
- Chronobiology
- DNA repair
- Environmental photobiology
- Nanotechnology in photobiology
- Photocarcinogenesis
- Photochemistry of biomolecules
- Photodynamic therapy
- Photomedicine
- Photomorphogenesis
- Photomovement
- Photoreception
- Photosensitization
- Photosynthesis
- Phototechnology
- Spectroscopy of biological systems
- UV and visible radiation effects and vision.