The use of chitosan as an antioxidant in the feed of cultivated P. vannamei shrimp against oxidative stress induced by exposure to microplastics

IF 3 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES Marine environmental research Pub Date : 2024-09-17 DOI:10.1016/j.marenvres.2024.106747

Chaelen Ortiz , Larissa Müller , Lucélia Borges , Luiz Antônio de Almeida Pinto , Tito Roberto Sant'anna Cadaval Jr. , Marcelo Borges Tesser , Virgínia Fonseca Pedrosa , Luis Alberto Romano , Wilson Wasielesky , Juliane Ventura-Lima

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Abstract

Microplastics (MP) are omnipresent in aquaculture and can induce several toxic effects, mainly oxidative stress. Therefore, alternatives to minimize these effects are welcome. In this study, chitosan (1 and 3 g/kg) was supplemented through the feed of farmed shrimp P. vannamei for 30 days. After this period, the shrimp were exposed to MP (0.5 mg/L) for 7 days. The results showed the presence of MP in hepatopancreas, gills and muscle. Hepatopancreas morphological alterations, as well as lipid peroxidation, a decrease in GSH level, and an increase in SOD activity indicated an oxidative stress that was reversed by chitosan. The muscle was also affected by MP, showing decreased CAT activity and increased SOD activity, though no lipid peroxidation was observed. In muscle, chitosan reversed the SOD increase to basal activity. The results obtained showed that chitosan was more effective against oxidative stress than in preventing accumulation and histological damage.

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在养殖的凡纳滨对虾饲料中使用壳聚糖作为抗氧化剂，以防止因接触微塑料而引起的氧化应激反应

微塑料（MP）在水产养殖中无处不在，可诱发多种毒性效应，主要是氧化应激。因此，尽量减少这些影响的替代品很受欢迎。在这项研究中，在养殖对虾（P. vannamei）的饲料中添加壳聚糖（1 和 3 克/千克），为期 30 天。之后，将对虾暴露于 MP（0.5 毫克/升）中 7 天。结果表明，MP 存在于肝胰腺、鳃和肌肉中。肝胰脏形态的改变、脂质过氧化、GSH 水平的下降和 SOD 活性的增加表明存在氧化应激，而壳聚糖可以逆转这种应激。肌肉也受到了 MP 的影响，CAT 活性降低，SOD 活性升高，但没有观察到脂质过氧化。在肌肉中，壳聚糖将 SOD 的增加逆转为基础活性。研究结果表明，壳聚糖对氧化应激的抑制作用要强于对蓄积和组织损伤的预防作用。

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来源期刊

Marine environmental research 环境科学-毒理学

CiteScore

5.90

自引率

3.00%

发文量

217

审稿时长

46 days

期刊介绍： Marine Environmental Research publishes original research papers on chemical, physical, and biological interactions in the oceans and coastal waters. The journal serves as a forum for new information on biology, chemistry, and toxicology and syntheses that advance understanding of marine environmental processes. Submission of multidisciplinary studies is encouraged. Studies that utilize experimental approaches to clarify the roles of anthropogenic and natural causes of changes in marine ecosystems are especially welcome, as are those studies that represent new developments of a theoretical or conceptual aspect of marine science. All papers published in this journal are reviewed by qualified peers prior to acceptance and publication. Examples of topics considered to be appropriate for the journal include, but are not limited to, the following: – The extent, persistence, and consequences of change and the recovery from such change in natural marine systems – The biochemical, physiological, and ecological consequences of contaminants to marine organisms and ecosystems – The biogeochemistry of naturally occurring and anthropogenic substances – Models that describe and predict the above processes – Monitoring studies, to the extent that their results provide new information on functional processes – Methodological papers describing improved quantitative techniques for the marine sciences.