Novel halotolerant PGPR strains alleviate salt stress by enhancing antioxidant activities and expression of selected genes leading to improved growth of Solanum lycopersicum

IF 3.9 2区农林科学 Q1 HORTICULTURE Scientia Horticulturae Pub Date : 2024-09-17 DOI:10.1016/j.scienta.2024.113625

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Abstract

Plant growth-promoting rhizobacteria (PGPR) offer an eco-friendly and sustainable option to alleviate salinity-induced plant changes. Two tomato varieties Rio Grande and Sahil were exposed to 150 mM NaCl stress in a completely randomized setup. The previously isolated strains Pseudarthrobacter oxydans and Staphylococcus pasteuri, best characterized for growth-promoting traits, were applied singly and in consortium to the salt-stressed tomato plants. Dry biomass, total protein content, enzymatic antioxidant activities, and non-enzymatic antioxidant production were measured. Expression of catalase (CAT), superoxide dismutase (SOD), and sodium-hydrogen antiporter (NHX3) genes was measured using qRT-PCR. Analysis of variance (ANOVA) followed by the Tukey HSD test was applied to compare mean values at p ≤ 0.05. PGPR inoculation increased the dry biomass and total protein content of both varieties. Furthermore, elevated CAT and SOD activities, DPPH scavenging, and production of phenolics and flavonoids were noted in tomato plants. Two-way ANOVA results showed a significant increase in the relative expression of selected genes in Rio Grande wherein a 3.47-fold increase in SOD, 2.52-fold in CAT, and 2.2-fold in NHX3 was noted as compared to NaCl-treated plants. The current study established that both strains have the potential to recover salinity-induced detrimental changes and may act as future biostimulants of the stressed plants.

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植物生长促进根瘤菌（PGPR）为缓解盐分引起的植物变化提供了一种生态友好和可持续的选择。在完全随机的设置中，两个番茄品种 Rio Grande 和 Sahil 暴露于 150 mM NaCl 胁迫下。在盐胁迫番茄植株上单独或联合施用了之前分离出的菌株 Pseudarthrobacter oxydans 和 Staphylococcus pasteuri，这两种菌株具有最佳的促进生长特性。测量了干生物量、总蛋白质含量、酶抗氧化活性和非酶抗氧化剂产量。过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和钠-氢反转运体（NHX3）基因的表达采用 qRT-PCR 技术进行测定。采用方差分析（ANOVA）和 Tukey HSD 检验比较平均值，p ≤ 0.05。接种 PGPR 增加了两个品种的干生物量和总蛋白质含量。此外，番茄植株的 CAT 和 SOD 活性、DPPH 清除能力以及酚类和黄酮类化合物的产量都有所提高。双向方差分析结果表明，与 NaCl 处理的植株相比，Rio Grande 中所选基因的相对表达量显著增加，其中 SOD 增加了 3.47 倍，CAT 增加了 2.52 倍，NHX3 增加了 2.2 倍。目前的研究表明，这两种菌株都具有恢复盐分引起的有害变化的潜力，未来可作为受胁迫植物的生物刺激剂。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.