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Differentiation of volatile organic compounds and sensory characteristics in the upper and lower flowers of grape hyacinth 葡萄风信子上下花挥发性有机物的分化及感官特征
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-17 DOI: 10.1016/j.scienta.2026.114633
Qian Lou , Chuhan Zhi , Jiayao Qin, Yishan Li, Hongwu Chen
Functional differentiation between sterile and fertile flowers is a key plant adaptation. While research has focused on visual traits, less attention has been given to volatile organic compounds (VOCs). Grape hyacinth (Muscari spp.), with its stable inflorescence pattern of fertile (open) lower and sterile (closed) upper flowers, is an ideal model for such study, yet its VOC composition remains uncharacterized. From ten initially screened species, three representative ones—M. azureum (M1), M. armeniacum ‘Heavenly Blue’ (M2), and M. aucheri ‘Ocean Magic’ (M3)—were selected based on highly significant differences in aroma intensity between upper and lower flowers. Analyses revealed spatial differentiation in VOCs and corresponding sensory attributes: upper flowers produced fewer compounds and were enriched in myrtenol and 1-octen-3-ol, potentially facilitating long-distance pollinator attraction, whereas lower flowers released more diverse VOCs that may prolong visitor retention. Myrtenol and 1-octen-3-ol established characteristic grassy and oily base notes across species. The three species developed distinct aromatic profiles through specific compounds: M1 accumulated ethyl phenylacetate with cocoa nuances; M2 contained abundant myrtenol and eucalyptol with minty tones; and M3 displayed fruity notes due to synergistic interaction between (Z)-β-ocimene and methyl salicylate. Furthermore, 1-octen-3-ol exhibited context-dependent expression, presenting earthy notes at Relative Odor Contribution (ROC) ≥ 99 % but shifting to herbaceous and oily tones at ROC < 50 %. In complex odor environments, myrtenol and acetophenone diverged from their typical minty and sweet-fruity profiles to instead contribute green nuances and modified minty characters. Additionally, sub-threshold ethyl phenylacetate helped shape the distinctive cocoa-fermented aroma in M1 through synergistic effects, further demonstrating how compound interactions dynamically modulate odor perception. This study demonstrates spatial differentiation of VOCs between upper and lower flowers in grape hyacinth and their potential ecological functions, providing new chemical insights into the evolution of floral traits and pollination strategies.
不育花和可育花的功能分化是植物适应的关键。虽然研究主要集中在视觉特征上,但对挥发性有机化合物(VOCs)的关注较少。葡萄风信子(Muscari spp.)具有稳定的花序模式,即下部可育(开放)和上部不育(封闭),是此类研究的理想模型,但其VOC组成尚未确定。从最初筛选的10个物种中,3个具有代表性的物种——m。根据上下花香气强度的显著差异,选择了azureum (M1), M. armeniacum ' Heavenly Blue ‘ (M2)和M. aucheri ’ Ocean Magic ' (M3)。分析结果表明,植物的挥发性有机化合物和相应的感官属性存在空间差异:上部花产生较少的化合物,富含桃金娘烯醇和1-辛烯-3-醇,可能有助于远距离吸引传粉者,而下部花释放更多的挥发性有机化合物,可能延长访问者的停留时间。桃金娘烯醇和1-辛烯-3-醇在不同物种间建立了典型的草味和油性基调。这三个物种通过特定的化合物形成了不同的芳香谱:M1积累了具有可可细微差别的苯乙酸乙酯;M2含有丰富的桃金娘烯醇和桉树精油,并带有薄荷色调;M3表现出果香,是由于(Z)-β-辛烯与水杨酸甲酯之间的协同作用。此外,1-辛烯-3-醇表现出上下文依赖的表达,在相对气味贡献(ROC)≥99%时呈现土调,而在ROC <; 50%时转向草本和油性调。在复杂的气味环境中,桃金娘烯醇和苯乙酮偏离了它们典型的薄荷味和甜果味特征,转而贡献绿色细微差别和修饰的薄荷特征。此外,亚阈值苯乙酸乙酯通过协同效应帮助形成M1中独特的可可发酵香气,进一步证明了化合物相互作用如何动态调节气味感知。本研究揭示了葡萄风信子上下花间挥发性有机化合物的空间分异及其潜在的生态功能,为花性状和传粉策略的进化提供了新的化学视角。
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引用次数: 0
Pear PbrPAO14 modulates pollen tube growth in vitro via altering tip-localized H₂O₂ content 梨PbrPAO14通过改变花粉管末端的H₂O₂含量调控离体花粉管生长
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-16 DOI: 10.1016/j.scienta.2026.114620
Yuzhen Qian, Haoran Wu, Lan Xu, Yuqian Wang, Kaijing Zhang, Yihu Sui, Xueping Zhang, Shuangshuang Jia, Nengbing Hu, Meilan Jiang, Yuxi Sun, Yingzhi Zhao, Ming Qian, Jingjing Qian
Polyamine oxidase (PAO)-derived hydrogen peroxide (H2O2) is known to participate in various plant growth and developmental processes. However, its role in pollen tube growth (PTG) of fruit trees remain elusive. In this study, we first identified 63 PAO members across four pear genomes, including 15 in Pyrus bretschneideri, and 16 each in P. betulifolia, P. pyrifolia, and P. communis. Phylogenetic analysis of 258 PAOs from 38 plant species classified them into seven distinct subclasses. The 63 pear PAOs were distributed across Subclasses I, III, IVa, IVb, and IVc, with members within each subclass exhibiting relatively conserved gene structures. Secondly, using P. bretschneideri as a model, tissue-specific expression analysis revealed that PbrPAO14 were specifically and highly expressed in pollen and pollen tube (PT), was selected for further functional characterization. Thirdly, transient suppression of the peroxisome-localized PbrPAO14 expression via antisense oligodeoxynucleotides significantly decreased PAO activity (by average 40 %), reduced apical H2O2 levels (by average 16 %) to increase PT length (by average 16 %). Furthermore, exogenous spermine and spermidine treatment markedly up-regulated PbrPAO14 expression, while its expression suppression led to significant spermine accumulation in PT. Recombinant His-PbrPAO14 protein preferentially used spermine as a substrate, converting spermine to spermidine and putrescine via back conversion (BC) pathway, and generating H2O2. Our results suggested that pear PbrPAO14 mainly regulates PTG by producing H2O2 via the BC pathway. This study advances our understanding of a PAO-mediated reactive oxygen species (ROS) source at the PT apex in the regulation of PTG, which significantly broadens the perspective on ROS dynamics in polarized cell growth.
多胺氧化酶(PAO)衍生的过氧化氢(H2O2)参与了植物的多种生长发育过程。然而,其在果树花粉管生长(PTG)中的作用尚不清楚。在这项研究中,我们首先在4个梨基因组中鉴定了63个PAO成员,其中梨(Pyrus bretschneideri) 15个,白花梨(P. betulifolia)、梨叶梨(P. pyrifolia)和社群梨(P. communis)各16个。对38种植物258个PAOs的系统发育分析将其划分为7个不同的亚纲。63个梨PAOs分布在I、III、IVa、IVb和IVc亚类中,每个亚类中的成员都表现出相对保守的基因结构。其次,以P. bretschneideri为模型,进行组织特异性表达分析,发现PbrPAO14在花粉和花粉管(PT)中特异性高表达,进一步进行功能表征。第三,通过反义寡脱氧核苷酸短暂抑制过氧化物酶体定位的PbrPAO14表达,显著降低PAO活性(平均降低40%),降低根尖H2O2水平(平均降低16%),增加PT长度(平均增加16%)。此外,外源精胺和亚精胺处理显著上调PbrPAO14的表达,而其表达抑制导致PT中显著的精胺积累。重组His-PbrPAO14蛋白优先以精胺为底物,通过BC途径将精胺转化为亚精胺和腐胺,并生成H2O2。我们的研究结果表明,梨PbrPAO14主要通过BC途径产生H2O2来调节PTG。本研究加深了我们对PTG调控中PT端活性氧(reactive oxygen species, ROS)来源的认识,大大拓宽了极化细胞生长过程中ROS动力学的研究视角。
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引用次数: 0
Identification of affinity of different pumpkin hybrid combinations with cucumber grafting 不同南瓜杂交组合与黄瓜嫁接的亲和力鉴定
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-16 DOI: 10.1016/j.scienta.2026.114629
Xue Lu, Meng Zhang, Mengchao Lei, Chunhui Wu, Weili Guo, Zhenwei Liu, Bihua Chen, Xinzheng Li
To study the grafting affinity of different pumpkin hybrid combinations with cucumber, this paper selected ten root-knot nematode-resistant and wilt-resistant pumpkin hybrid combinations as rootstocks for cucumber grafting. Grafting survival rate, growth status of grafted seedlings, root system vitality, enzyme activity, and other indicators were measured. The results showed that among the ten pumpkin hybrid combinations, grafted cucumber seedlings exhibited superior growth indicators and higher CAT activity compared to self-rooted cucumber; The root activity and the SOD activity of nine pumpkin hybrid combinations grafted cucumber were higher than that of self-rooted cucumber; the POD activity of three pumpkin hybrid combinations grafted cucumber was significantly higher than that of self-rooted cucumber; and the MDA content of seven pumpkin hybrid combinations of grafted cucumber was lower than that of self-rooted cucumber. The above results indicated that grafting may reduce membrane lipid peroxidation by increasing the activity of protective enzymes in cucumber, thereby enhancing their resistance and improving their adaptability to a certain extent. The analysis of the membership function showed that the pumpkin hybrid combinations showing good performance before and after grafting were 487–2 × 112–2, 487–2 × LingchuanC1, Renhe-1 × LingchuanC1, and Renhe-1 × Daziwang-24. Analysis of enzyme activities at the junction of the rootstock and the scion indicated that the pumpkin hybrid combinations Fangshan × Renhe-1, 487–2 × 112–2, 487–2 × LingchuanC1, and Renhe-1 × LingchuanC1 grafted cucumber had lower callose synthase activity than other pumpkin hybrid combinations. Comprehensive membership function analysis indicated that the pumpkin hybrid combinations 487–2 × 112–2, 487–2 × LingchuanC1 and Renhe-1 × LingchuanC1 grafted cucumber have excellent grafting affinity advantage.
为研究不同南瓜杂交组合与黄瓜的嫁接亲和性,选择10个抗根结线虫和抗枯萎的南瓜杂交组合作为黄瓜嫁接的砧木。测定嫁接成活率、嫁接苗生长状况、根系活力、酶活性等指标。结果表明:在10个南瓜杂交组合中,嫁接黄瓜幼苗的生长指标优于自根黄瓜,CAT活性高于自根黄瓜;嫁接黄瓜的9个南瓜杂交组合根系活力和SOD活力均高于自根黄瓜;3个南瓜杂交组合嫁接黄瓜的POD活性均显著高于自根黄瓜;嫁接黄瓜7个南瓜杂交组合的丙二醛含量均低于自根黄瓜。上述结果表明,嫁接可以通过增加黄瓜保护酶的活性来减少膜脂过氧化,从而在一定程度上增强黄瓜的抗性,提高黄瓜的适应性。隶属函数分析表明,嫁接前后表现较好的南瓜杂交种组合为487-2 × 112-2、487-2 ×灵川1号、仁和1号×灵川1号和仁和1号×大之王24。砧木与接穗处酶活性分析表明,“房山”ד仁和”1、“487-2”ד112-2”、“487-2”ד凌川”1和“仁和”1 ד凌川”1嫁接黄瓜的南瓜杂交组合的钙酶活性低于其他南瓜杂交组合。综合隶属函数分析表明,487-2 × 112-2、487-2 ×凌川1号和仁和1号×凌川1号嫁接黄瓜具有优异的嫁接亲和优势。
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引用次数: 0
Suitability of Apium graveolens L. var. secalinum Alef. to hydroponic cultivation for baby leaf production 荆芥叶的适宜性。以水培法生产幼叶
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-16 DOI: 10.1016/j.scienta.2026.114619
Alessandro Esposito, Filippo Vetrano, Alessandra Moncada, Eristanna Palazzolo, Caterina Lucia, Alessandro Miceli
Consumers are increasingly seeking innovative, healthy foods rich in nutraceuticals, driving the search for new or underutilized leafy vegetables. Leaf celery (Apium graveolens L. var. secalinum Alef.), a promising candidate for new food sources, stands out from ribbed celery with its smaller size and enhanced aroma. It is gaining global interest due to its high concentration of bioactive compounds, but it is presently cultivated only on soil in restricted regions. Significant knowledge gaps still exist regarding optimal agronomic management for its hydroponic baby leaf production and post-harvest cold storage as a minimally processed product. The necessity of adopting hydroponic systems for ready-to-eat leafy salads requires specific studies on techniques and nutrient management for novel vegetables like leaf celery. This research, for the first time, investigates the feasibility of producing fresh-cut suitable leaf celery baby leaves using a hydroponic ebb-and-flow cultivation system. We studied the effects of two plant densities (615 and 947 plants m⁻²) and three nutrient solution concentrations (NS) (only water, half strength, and full strength) on leaf celery growth, yield, and postharvest quality over two growing seasons (S1: winter/spring and S2: spring/summer). The experiment included two mowings per season to test the plant's regrowth capability, with morphological, biochemical, and yield characteristics assessed after each. Leaves from the first mowing of each trial were tested via sensory analysis and evaluated for shelf-life following minimal processing and cold storage (21 days at 4 °C). This research provides essential, globally transferable data for sustainable Controlled Environment Agriculture (CEA) by quantifying the yield, nutritional stability, and post-harvest longevity of this novel crop across critical seasonal and resource management variables. Results showed higher total yields in S1 (5.25 kg m⁻²) compared to S2 (2.76 kg m⁻²) using the full-strength NS, with nutrient availability effects varying by season and density. The full-strength NS maximized total yield, while the half-strength NS achieved the highest NUE (35.6 g DW g−1 N in S1). Importantly, the baby leaves exhibited good vitamin and mineral content with consistent stability across growing seasons and mowings. Their sensory profile showed only minor differences between seasons, generally maintaining a good overall evaluation. Crucially, the leaves maintained a shelf-life exceeding 14 days across all tested treatments. Overall, leaf celery proved well-suited for hydroponic cultivation, yielding baby leaves with excellent shelf-life and nutritional quality, offering a viable high-value option for the fresh-cut market.
消费者越来越多地寻求富含营养药品的创新健康食品,推动了对新的或未充分利用的叶菜的寻找。叶芹菜(Apium graveolens L. var. secalinum Alef.)是一种很有前途的新食物来源,它以其较小的体积和增强的香气在肋芹菜中脱颖而出。由于其高浓度的生物活性化合物,它正在获得全球的兴趣,但目前只在限制地区的土壤上种植。显著的知识差距仍然存在关于其水培幼叶生产和收获后冷藏作为最低限度加工产品的最佳农艺管理。采用水培系统生产即食叶沙拉的必要性要求对叶芹菜等新型蔬菜的技术和营养管理进行专门的研究。本研究首次探讨了利用水培潮落栽培系统生产鲜切适叶芹菜幼叶的可行性。我们研究了两种种植密度(615株和947株m - 2)和三种营养液浓度(NS)(纯水、一半强度和全部强度)在两个生长季节(S1:冬季/春季和S2:春季/夏季)对芹菜叶片生长、产量和采后品质的影响。试验包括每季两次刈割,以测试植物的再生能力,每次刈割后评估形态、生化和产量特征。每个试验的第一次刈割的叶片通过感官分析进行测试,并在最小加工和冷藏(4°C下21天)后评估其保质期。本研究通过量化这种新型作物在关键季节和资源管理变量中的产量、营养稳定性和收获后寿命,为可持续受控环境农业(CEA)提供了重要的、全球可转移的数据。结果显示,使用全强度NS的S1 (5.25 kg m -⁻²)比S2 (2.76 kg m -⁻²)的总产量更高,营养物质的可用性随季节和密度而变化。全强度氮肥的总产量最高,而半强度氮肥的氮肥利用率最高,为35.6 g DW g−1 N。重要的是,幼叶表现出良好的维生素和矿物质含量,在整个生长季节和修剪期间都保持稳定。他们的感官特征在季节之间只有微小的差异,总体上保持良好的整体评价。至关重要的是,在所有测试处理中,叶子的保质期都超过了14天。总的来说,叶芹菜非常适合水培栽培,产生的幼叶具有良好的保质期和营养质量,为新鲜切割市场提供了可行的高价值选择。
{"title":"Suitability of Apium graveolens L. var. secalinum Alef. to hydroponic cultivation for baby leaf production","authors":"Alessandro Esposito,&nbsp;Filippo Vetrano,&nbsp;Alessandra Moncada,&nbsp;Eristanna Palazzolo,&nbsp;Caterina Lucia,&nbsp;Alessandro Miceli","doi":"10.1016/j.scienta.2026.114619","DOIUrl":"10.1016/j.scienta.2026.114619","url":null,"abstract":"<div><div>Consumers are increasingly seeking innovative, healthy foods rich in nutraceuticals, driving the search for new or underutilized leafy vegetables. Leaf celery (<em>Apium graveolens</em> L<em>.</em> var. <em>secalinum</em> Alef.), a promising candidate for new food sources, stands out from ribbed celery with its smaller size and enhanced aroma. It is gaining global interest due to its high concentration of bioactive compounds, but it is presently cultivated only on soil in restricted regions. Significant knowledge gaps still exist regarding optimal agronomic management for its hydroponic baby leaf production and post-harvest cold storage as a minimally processed product. The necessity of adopting hydroponic systems for ready-to-eat leafy salads requires specific studies on techniques and nutrient management for novel vegetables like leaf celery. This research, for the first time, investigates the feasibility of producing fresh-cut suitable leaf celery baby leaves using a hydroponic ebb-and-flow cultivation system. We studied the effects of two plant densities (615 and 947 plants m⁻²) and three nutrient solution concentrations (NS) (only water, half strength, and full strength) on leaf celery growth, yield, and postharvest quality over two growing seasons (S1: winter/spring and S2: spring/summer). The experiment included two mowings per season to test the plant's regrowth capability, with morphological, biochemical, and yield characteristics assessed after each. Leaves from the first mowing of each trial were tested via sensory analysis and evaluated for shelf-life following minimal processing and cold storage (21 days at 4 °C). This research provides essential, globally transferable data for sustainable Controlled Environment Agriculture (CEA) by quantifying the yield, nutritional stability, and post-harvest longevity of this novel crop across critical seasonal and resource management variables. Results showed higher total yields in S1 (5.25 kg m⁻²) compared to S2 (2.76 kg m⁻²) using the full-strength NS, with nutrient availability effects varying by season and density. The full-strength NS maximized total yield, while the half-strength NS achieved the highest NUE (35.6 g DW g<sup>−1</sup> N in S1). Importantly, the baby leaves exhibited good vitamin and mineral content with consistent stability across growing seasons and mowings. Their sensory profile showed only minor differences between seasons, generally maintaining a good overall evaluation. Crucially, the leaves maintained a shelf-life exceeding 14 days across all tested treatments. Overall, leaf celery proved well-suited for hydroponic cultivation, yielding baby leaves with excellent shelf-life and nutritional quality, offering a viable high-value option for the fresh-cut market.</div></div>","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"357 ","pages":"Article 114619"},"PeriodicalIF":4.2,"publicationDate":"2026-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrating UAV multi-sensor imagery and machine learning for non-destructive estimation of nitrogen nutrition index in tea plants 基于无人机多传感器图像和机器学习的茶树氮素营养指数无损估计
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-16 DOI: 10.1016/j.scienta.2026.114614
Zhipeng Li , Shuting Gong , Xinle Jiang , Wentian Yang , Wenmei Wang , WenJun Qian , Yu Wang , Zhaotang Ding , Kai Fan
Accurate diagnosis of nitrogen status is globally crucial for precise fertilization and optimal management in tea plantations. The Nitrogen Nutrition Index (NNI) provides a robust quantitative measure of N sufficiency, yet its practical application in tea plants has been limited due to the lack of established critical nitrogen dilution curves (CNDC) and validated non‑destructive monitoring methods. To address this, this study developed a novel UAV‑based multi‑source remote sensing approach for NNI estimation in tea plants, innovatively combining LiDAR with multispectral (MS) and visible‑light (RGB) data to capture complementary structural and spectral information. Field experiments involving three tea varieties under six N treatments were conducted, and NNI estimation models were built by integrating three feature selection methods (Boruta, RFE, ElasticNet) with four machine learning algorithms (SVM, PLS, RF, GPR). The results demonstrated a significant nitrogen fertilization effect, with NNI values ranging from 0.36 to 1.16, increasing with nitrogen application. Single-source remote sensing features selected by the three algorithms exhibited the performance ranking of MS > RGB > LiDAR. Multi‑sensor feature fusion significantly outperformed single‑sensor models. The optimal model, integrating Boruta-selected MS+RGB features with GPR, achieved the highest accuracy (R2=0.7346, RMSE=0.0878, RPD=1.6987). This model provides an accurate and non‑destructive tool for field‑scale monitoring of tea nitrogen status, supporting precision fertilization that can reduce fertilizer application in tea cultivation.
准确诊断茶园氮素状况对茶园精准施肥和优化管理具有重要意义。氮营养指数(NNI)提供了一个强有力的定量衡量氮充分性的指标,但由于缺乏建立的临界氮稀释曲线(CNDC)和经过验证的非破坏性监测方法,其在茶树中的实际应用受到限制。为了解决这一问题,本研究开发了一种新的基于无人机的多源遥感方法,用于估算茶树的NNI,创新地将激光雷达与多光谱(MS)和可见光(RGB)数据相结合,以捕获互补的结构和光谱信息。以3个茶叶品种为研究对象,在6种氮处理下进行田间试验,将3种特征选择方法(Boruta、RFE、ElasticNet)与4种机器学习算法(SVM、PLS、RF、GPR)相结合,建立NNI估计模型。结果表明,施氮效果显著,NNI值在0.36 ~ 1.16之间,随施氮量的增加而增加。三种算法选择的单源遥感特征均表现出MS >; RGB >; LiDAR的性能排名。多传感器特征融合显著优于单传感器模型。将boruta选择的MS+RGB特征与GPR相结合的最优模型准确率最高(R2=0.7346, RMSE=0.0878, RPD=1.6987)。该模型为茶叶氮素状况的田间监测提供了一种准确、无损的工具,可为精准施肥提供支持,减少茶叶种植过程中的化肥用量。
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引用次数: 0
Modeling heat requirements for fruit development in three pistachio cultivars in central Spain 西班牙中部三个开心果品种果实发育的热需求模拟
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-16 DOI: 10.1016/j.scienta.2026.114634
Sara Álvarez , Pablo Garcia-Estringana , Abel Barrios , Jesús Alegre , Lidia Núñez , Laura Salcedo , Hugo Martín , Eduardo Fernández-Suela , Noelia Ramírez-Martín , José Manuel Mirás-Avalos
Pistachio (Pistacia vera L.) is a crop with a high economic importance that is expanding in the Mediterranean basin. Understanding how pistachio growth stages vary with environmental conditions is crucial because decisions about irrigation, fertilization, and pest control must align with tree physiological needs at each stage. However, a description of the phenological stages of pistachio is lacking in the areas covered by this study. In this study, the growth of the fruit and kernel were measured in three pistachio cultivars: ‘Kerman’, ‘Golden Hills’ and ‘Lost Hills’ in six plots located in central Spain to establish predictive models based on heat accumulation. The growing degree days (GDD) necessary to reach 90% full fruit area were similar for the three cultivars, around 450 GDD. In contrast, the thermal units required to reach 90% of full kernel area and length differed among cultivars: ‘Kerman’ had the highest needs, while ‘Lost Hills’ had the lowest. Fruit dry weight started at 200 GDD and continued until 2000 GDD, whereas kernel area started its expansion at 900 and continued until 2000 GDD. These findings suggest that deficit irrigation could be applied between 450 and 900 GDD, without compromising pistachio yield. A model previously proposed in California underestimated both fruit volume and kernel length when applied to our calibration dataset. Conversely, the model calibrated in the current work performed better and can be considered satisfactory for assessing kernel length and fruit volume as a function of cumulative GDD. These results provide essential insights into the phenological development of pistachio growing in cold-winter regions, aiding farmers to take informed decisions for an efficient orchard management.
开心果(Pistacia vera L.)是一种具有高度经济重要性的作物,在地中海盆地正在扩大。了解开心果生长阶段如何随环境条件而变化是至关重要的,因为有关灌溉、施肥和虫害防治的决定必须与树木在每个阶段的生理需求保持一致。然而,在本研究覆盖的地区,对开心果物候阶段的描述是缺乏的。本研究以位于西班牙中部的六个样地的“Kerman”、“Golden Hills”和“Lost Hills”三个开心果品种的果实和果仁的生长情况进行了测量,建立了基于热量积累的预测模型。3个品种达到90%满果面积所需的生长度日(GDD)相似,均在450 GDD左右。相比之下,达到90%全粒面积和长度所需的热量单位在不同品种之间存在差异:“Kerman”的需求最高,而“Lost Hills”的需求最低。果实干重从200 GDD开始并持续到2000 GDD,而果仁面积从900 GDD开始扩张并持续到2000 GDD。这些发现表明,亏缺灌溉可以在450 - 900 GDD之间施用,而不会影响开心果产量。先前在加州提出的模型在应用于我们的校准数据集时低估了果实体积和果仁长度。相反,在当前工作中校准的模型表现更好,可以认为令人满意地评估果核长度和果实体积作为累积GDD的函数。这些结果为了解寒冷冬季地区开心果生长的物候发展提供了重要的见解,帮助农民做出明智的决策,实现高效的果园管理。
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引用次数: 0
Silicon mitigates copper toxicity of Citrus sinensis (L.) by improving nutrient status, root development, photosynthetic performance and antioxidant response 硅通过改善柑橘的营养状况、根系发育、光合性能和抗氧化反应来减轻铜毒性
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-15 DOI: 10.1016/j.scienta.2026.114622
Zeng-Rong Huang, Wenqing Shi, Xing Xiong, Yu Zhou, Fei Lu, Faxin Li, Yu Chen, Ningwei Lai, Li-Song Chen
China is the world's largest producer of citrus fruits. However, soil acidification and irrational copper (Cu) application in citrus orchards frequently induce Cu toxicity, restricting the sustainable development of the citrus industry. Studies have shown that silicon (Si) fertilization effectively mitigates the toxicity of heavy metals. However, research on Si-mediated alleviation of Cu toxicity in citrus species remains limited. In the present study, sand-cultured seedlings of sweet orange [Citrus sinensis (L) Osbeck Cv. Xuegan] were subjected to eight treatments containing 2 Cu concentrations (0.5 μM and 300 μM) and 4 Si concentrations (0, 1, 3, and 5 mM). The effects of those treatments on biomass accumulation, Cu and Si distribution, root architecture, nutrient uptake, leaf photosynthetic performance and antioxidant systems were investigated to reveal the physiological and biochemical mechanisms underlying Si-mediated alleviation of Cu toxicity in C. sinensis seedlings. The present results verified that supplementation with Si at an optimal level promoted C. sinensis Cu tolerance by decreasing Cu translocation, maintaining nutrient homeostasis, improving photosynthetic performance, and activating the antioxidant defense in C. sinensis. Overall, the present results indicated that 3 mM Si is more beneficial against 300 μM Cu stress in C. sinensis seedlings. The results identified the benefits of Si in woody plants and will provide a necessary reference for Cu detoxification in acidified citrus orchards of South China.
中国是世界上最大的柑橘类水果生产国。然而,柑橘园土壤酸化和不合理的铜施用往往诱发铜中毒,制约了柑橘产业的可持续发展。研究表明,施硅能有效减轻重金属的毒性。然而,关于硅介导的柑橘铜毒性缓解的研究仍然有限。本研究以甜橙(Citrus sinensis (L) Osbeck Cv.;雪柑受到2种Cu浓度(0.5 μM和300 μM)和4种Si浓度(0、1、3和5 mM)的8种处理。研究了不同处理对紫杉树生物量积累、Cu和Si分布、根系构型、养分吸收、叶片光合性能和抗氧化系统的影响,揭示了硅介导的紫杉树幼苗Cu毒性缓解的生理生化机制。本研究结果证实,添加最佳水平的Si可通过减少铜转运、维持营养平衡、提高光合性能和激活抗氧化防御等方式促进中华金银花对铜的耐受。综上所述,3 mM Si对300 μM Cu胁迫下紫杉树幼苗更有利。研究结果表明,Si对木本植物有益,可为华南酸化柑橘的Cu脱毒提供必要的参考。
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引用次数: 0
Irrigation and fertilization improved vegetative growth and fruit development of winter jujube in plastic shed production through enhanced photosynthetic capacity and nutrient uptake 灌溉和施肥通过提高光合能力和养分吸收来促进塑料大棚生产冬枣的营养生长和果实发育
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-15 DOI: 10.1016/j.scienta.2026.114616
Xiaoqiang Liu , Fuquan Lv , Xiaoshu Pan , Ying Qu , Lei Shi , Shuai He , Fucang Zhang , Feihu Yin
<div><div>Understanding the nutritional status and photosynthetic performance of orchard crops under different irrigation and fertilization regimes is crucial for optimizing vegetative growth and promoting fruit development. To address this, the present study aimed to explore the relationships among growth indicators, photosynthetic parameters (photosynthetic rate: Pn, stomatal conductance: g<sub>s</sub>, transpiration rate: Tr, instantaneous water use efficiency: WUE<sub>i</sub>), nutrients uptake (total nitrogen: N, total phosphorus: P, total potassium: K), and fruit development and weight based on structural equation modeling (SEM) under varied irrigation and fertilization treatments, and develop the appropriate irrigation and fertilization management strategy for sustainable winter jujube production in plastic shed. A two-year field experiment on winter jujube irrigation and fertilization management was conducted in southern Xinjiang, China, during 2023 and 2024, involving 16 drip-fertigation treatments. Treatments were as follows: four irrigation amounts (W<sub>1</sub>: 60% ETc, W<sub>2</sub>: 80% ETc, W<sub>3</sub>: 100% ET<sub>C</sub>, and W<sub>4</sub>: 120% ETc) and four fertilization rates (N-P-K, F<sub>1</sub>: 192-96-150 kg ha<sup>−1</sup>, F<sub>2</sub>: 256-128-200 kg ha<sup>−1</sup>, F<sub>3</sub>: 320-160-250 kg ha<sup>−1</sup>, and F<sub>4</sub>: 384-192-300 kg ha<sup>−1</sup>). Results indicated that W<sub>2</sub> improved the N content of stems, leaves and fruits compared with W<sub>1</sub>. The F<sub>3</sub> could increase the P content and K content of fruits. The Tr was more sensitive to water and fertilizer than the Pn, resulting higher the WUE<sub>i</sub>. Irrigation and fertilization had extremely significant effects on pruning amounts (<em>P</em> < 0.01), while the relative chlorophyll content (SPAD) exhibited a decreasing tendency as the irrigation amount increased. The W<sub>2</sub>F<sub>2</sub> could inhibit excessive vegetative growth while promoting fruit development. Moreover, the Logistic equation fitting for fruit volume revealed that the start time (<em>t</em><sub>1</sub>) of the rapid fruit volume accumulation period range from 55.47 to 71.59 days after flowering (DAF), and the end time (<em>t</em><sub>2</sub>) of the rapid fruit volume accumulation period range from 82.96 to 114.17 DAF in both years. The fresh fruit weight increased and then declined with the increase of fertilization rates. The SEM analysis revealed that the N content was the primary influencing factor for fruit volume and weight, with standardized path coefficient of 0.836 and 0.715, respectively (both <em>P</em> < 0.05). Additionally, growth indicators (e.g., new shoot length) exerted an indirect negative effect on fresh fruit weight. Collectively, irrigation and fertilizer can promote vegetative growth and fruit development of winter jujube by enhancing photosynthetic capacity and nutrient uptake, highlighting their critical role in
了解不同灌溉和施肥制度下果园作物的营养状况和光合性能对优化营养生长和促进果实发育具有重要意义。为解决这一问题,本研究旨在探讨生长指标、光合参数(光合速率:Pn、气孔导度:gs、蒸腾速率:Tr、瞬时水分利用效率:WUEi)、养分吸收(全氮:N、全磷:P、全钾:基于结构方程模型(SEM)对不同水肥处理下冬枣果实发育和质量的影响,制定适宜的水肥管理策略,实现塑料大棚冬枣可持续生产。于2023年和2024年在南疆地区进行了为期两年的冬枣灌溉施肥管理田间试验,共16个滴灌施肥处理。处理为4种灌水量(W1: 60%等,W2: 80%等,W3: 100%等,W4: 120%等)和4种施肥水平(N-P-K, F1: 192 ~ 96 ~ 150 kg ha -1, F2: 256 ~ 128 ~ 200 kg ha -1, F3: 320 ~ 160 ~ 250 kg ha -1, F4: 384 ~ 192 ~ 300 kg ha -1)。结果表明,与W1相比,W2提高了茎秆、叶片和果实的氮含量。F3能提高果实的磷、钾含量。Tr对水肥比Pn更敏感,导致WUEi更高。灌溉和施肥对剪枝量的影响极显著(P < 0.01),而相对叶绿素含量(SPAD)随灌水量的增加呈下降趋势。W2F2在促进果实发育的同时抑制营养生长过度。果实体积的Logistic方程拟合结果表明,两年内果实体积快速积累期的开始时间(t1)为开花后55.47 ~ 71.59天,果实体积快速积累期的结束时间(t2)为82.96 ~ 114.17天。鲜果重随施肥量的增加先上升后下降。SEM分析显示,氮含量是影响果实体积和重量的主要因素,其标准化通径系数分别为0.836和0.715 (P < 0.05)。此外,生长指标(如新梢长)对鲜果重有间接的负影响。综上所述,灌溉和施肥可以通过提高冬枣的光合能力和养分吸收来促进冬枣的营养生长和果实发育,突出了它们在冬枣栽培中的关键作用。
{"title":"Irrigation and fertilization improved vegetative growth and fruit development of winter jujube in plastic shed production through enhanced photosynthetic capacity and nutrient uptake","authors":"Xiaoqiang Liu ,&nbsp;Fuquan Lv ,&nbsp;Xiaoshu Pan ,&nbsp;Ying Qu ,&nbsp;Lei Shi ,&nbsp;Shuai He ,&nbsp;Fucang Zhang ,&nbsp;Feihu Yin","doi":"10.1016/j.scienta.2026.114616","DOIUrl":"10.1016/j.scienta.2026.114616","url":null,"abstract":"&lt;div&gt;&lt;div&gt;Understanding the nutritional status and photosynthetic performance of orchard crops under different irrigation and fertilization regimes is crucial for optimizing vegetative growth and promoting fruit development. To address this, the present study aimed to explore the relationships among growth indicators, photosynthetic parameters (photosynthetic rate: Pn, stomatal conductance: g&lt;sub&gt;s&lt;/sub&gt;, transpiration rate: Tr, instantaneous water use efficiency: WUE&lt;sub&gt;i&lt;/sub&gt;), nutrients uptake (total nitrogen: N, total phosphorus: P, total potassium: K), and fruit development and weight based on structural equation modeling (SEM) under varied irrigation and fertilization treatments, and develop the appropriate irrigation and fertilization management strategy for sustainable winter jujube production in plastic shed. A two-year field experiment on winter jujube irrigation and fertilization management was conducted in southern Xinjiang, China, during 2023 and 2024, involving 16 drip-fertigation treatments. Treatments were as follows: four irrigation amounts (W&lt;sub&gt;1&lt;/sub&gt;: 60% ETc, W&lt;sub&gt;2&lt;/sub&gt;: 80% ETc, W&lt;sub&gt;3&lt;/sub&gt;: 100% ET&lt;sub&gt;C&lt;/sub&gt;, and W&lt;sub&gt;4&lt;/sub&gt;: 120% ETc) and four fertilization rates (N-P-K, F&lt;sub&gt;1&lt;/sub&gt;: 192-96-150 kg ha&lt;sup&gt;−1&lt;/sup&gt;, F&lt;sub&gt;2&lt;/sub&gt;: 256-128-200 kg ha&lt;sup&gt;−1&lt;/sup&gt;, F&lt;sub&gt;3&lt;/sub&gt;: 320-160-250 kg ha&lt;sup&gt;−1&lt;/sup&gt;, and F&lt;sub&gt;4&lt;/sub&gt;: 384-192-300 kg ha&lt;sup&gt;−1&lt;/sup&gt;). Results indicated that W&lt;sub&gt;2&lt;/sub&gt; improved the N content of stems, leaves and fruits compared with W&lt;sub&gt;1&lt;/sub&gt;. The F&lt;sub&gt;3&lt;/sub&gt; could increase the P content and K content of fruits. The Tr was more sensitive to water and fertilizer than the Pn, resulting higher the WUE&lt;sub&gt;i&lt;/sub&gt;. Irrigation and fertilization had extremely significant effects on pruning amounts (&lt;em&gt;P&lt;/em&gt; &lt; 0.01), while the relative chlorophyll content (SPAD) exhibited a decreasing tendency as the irrigation amount increased. The W&lt;sub&gt;2&lt;/sub&gt;F&lt;sub&gt;2&lt;/sub&gt; could inhibit excessive vegetative growth while promoting fruit development. Moreover, the Logistic equation fitting for fruit volume revealed that the start time (&lt;em&gt;t&lt;/em&gt;&lt;sub&gt;1&lt;/sub&gt;) of the rapid fruit volume accumulation period range from 55.47 to 71.59 days after flowering (DAF), and the end time (&lt;em&gt;t&lt;/em&gt;&lt;sub&gt;2&lt;/sub&gt;) of the rapid fruit volume accumulation period range from 82.96 to 114.17 DAF in both years. The fresh fruit weight increased and then declined with the increase of fertilization rates. The SEM analysis revealed that the N content was the primary influencing factor for fruit volume and weight, with standardized path coefficient of 0.836 and 0.715, respectively (both &lt;em&gt;P&lt;/em&gt; &lt; 0.05). Additionally, growth indicators (e.g., new shoot length) exerted an indirect negative effect on fresh fruit weight. Collectively, irrigation and fertilizer can promote vegetative growth and fruit development of winter jujube by enhancing photosynthetic capacity and nutrient uptake, highlighting their critical role in ","PeriodicalId":21679,"journal":{"name":"Scientia Horticulturae","volume":"357 ","pages":"Article 114616"},"PeriodicalIF":4.2,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145963373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimizing kiwifruit cultivar selection through integrated quality evaluation: An entropy weighted-analytic hierarchy process approach for balancing biochemical and sensory attributes 通过综合品质评价优化猕猴桃品种选择:一种平衡生化和感官属性的熵权层次分析法
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-15 DOI: 10.1016/j.scienta.2026.114618
Zhu Gao , Dan Zhang , Jipeng Mao , Yukun Zhu , Dongliang Yao , Mengfei Lin , Liuyi Pan , Xiaoling Wang , Lu Chen
As a high-value fruit, kiwifruit’s quality characteristics are jointly influenced by cultivar and production environment. However, current issues in China’s main production regions, such as a narrow cultivar structure and insufficient regional distinctiveness, highlight the urgent need for systematic evaluation of quality differences among cultivars to guide industrial optimization. This study investigated 32 commercially cultivated kiwifruit varieties grown in same climatic region, conducting statistical analysis and comprehensive evaluation on 20 quality indicators encompassing fruit appearance, flavor, and nutritional attributes. The results revealed significant differences in fruit quality both between and within species. Objective evaluation methods (membership function principal component analysis, and entropy weight) and a combined subjective-objective approach (entropy weighted-analytic hierarchy process, EW-AHP) were employed to establish comprehensive fruit quality evaluation models. Among these, the EW-AHP model exhibited the highest consistency with sensory evaluations, confirming its reliability. According to the EW-AHP model, ‘Hongyang’, ‘Fenghuang 1′, and ‘Cuiyu’ ranked highest due to their high sugar and low acid content, securing the top three positions. Seven other cultivars—‘Donghong’, ‘Hongshi 2′, ‘Huayou’, ‘Qihong’, ‘Lushanxiang’, ‘Yunhai 1′, and ‘Cuixiang’—also ranked within the top ten owing to their superior flavor and nutritional profiles. In contrast, cultivars such as ‘Jintao’, despite their appealing appearance, scored lower in comprehensive evaluation due to their high acidity. The proposed EW-AHP method effectively mitigates biases inherent in single evaluation approaches, providing a scientific basis for regional cultivar selection and precise alignment with consumer markets. This study holds significant implications for advancing the high-quality development of the kiwifruit industry.
猕猴桃作为一种高价值水果,其品质特性受品种和生产环境的共同影响。然而,目前中国主要产区存在品种结构狭窄、区域特色不足等问题,迫切需要对品种间质量差异进行系统评价,以指导产业优化。本研究以同一气候区域32个商品栽培猕猴桃品种为研究对象,对果实外观、风味、营养等20项品质指标进行了统计分析和综合评价。结果表明,种内和种间果实品质差异显著。采用隶属函数主成分分析法、熵权分析法等客观评价方法和主客观相结合的熵权层次分析法建立了水果品质综合评价模型。其中EW-AHP模型与感官评价的一致性最高,证实了其可靠性。根据EW-AHP模型,“红阳”、“凤凰1号”和“翠玉”因其高糖低酸含量排名最高,占据了前三名的位置。其他7个品种——‘东红’、‘红石2号’、‘花优’、‘启红’、‘芦山香’、‘云海1号’和‘翠香’也因其优异的风味和营养成分进入了前十名。​提出的EW-AHP方法有效地减轻了单一评价方法固有的偏差,为区域品种选择和精确定位消费者市场提供了科学依据。本研究对推动猕猴桃产业的高质量发展具有重要意义。
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引用次数: 0
Regulation of nectarine flesh browning through pre-harvest application of chitosan coatings 收获前应用壳聚糖涂层调控油桃果肉褐变
IF 4.2 2区 农林科学 Q1 HORTICULTURE Pub Date : 2026-01-15 DOI: 10.1016/j.scienta.2026.114613
Zhu Li , Xupeng Zeng , Yue Chen , Huihuang Shi , Biting Shao , Rui Gong , Zhongjing Li
Nectarine (Prunus persica L.) is a popular economic fruit, but flesh browning during low-temperature cold storage severely limits its storage period and commercial value, with existing studies on pre-harvest chitosan treatment focusing on single metabolic pathways. This study explored the regulatory effect and mechanism of pre-harvest 1.5 g/L chitosan spraying (4 times, 7-day interval) combined with (0±0.5)°C cold storage on 'Zhongyou No.4′ nectarine browning. Results showed the treatment delayed browning onset from week 2 to 3, reduced browning index, and maintained fruit quality. Mechanistically, it synergistically regulated multiple metabolic pathways: preserving mitochondrial function for energy supply, balancing mineral elements (K, Ca, B) to stabilize cell structure, inhibiting cell wall-degrading enzymes, maintaining sucrose-hexose balance, and enhancing ROS scavenging capacity. This work fills the gap of multi-metabolic network regulation research, providing a safe and effective technical and theoretical basis for nectarine cold storage preservation.
油桃(Prunus persica L.)是一种受欢迎的经济水果,但低温冷藏过程中的果肉褐变严重限制了其储存期和商业价值,目前对采前壳聚糖处理的研究主要集中在单一代谢途径上。本研究探讨了采前1.5 g/L壳聚糖喷施(4次,间隔7 d)联合(0±0.5)℃冷藏对“中油4号”油桃褐变的调控作用及其机理。结果表明:处理后褐变发生时间从第2周推迟到第3周,褐变指数降低,果实品质保持不变。在机制上,它协同调节多种代谢途径:维持线粒体功能以维持能量供应,平衡矿物质元素(K、Ca、B)以稳定细胞结构,抑制细胞壁降解酶,维持蔗糖-己糖平衡,增强活性氧清除能力。本工作填补了多代谢网络调控研究的空白,为油桃冷库保鲜提供了安全有效的技术和理论依据。
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引用次数: 0
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Scientia Horticulturae
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