Genetic variability of Myxobolus nagaraensis (Bivalvulida: Myxobolidae) infecting freshwater gobies Rhinogobius Gill 1859 (Gobiiformes: Oxudercidae) from rivers in Japan

IF 2 3区医学 Q3 ECOLOGY International Journal for Parasitology-Parasites and Wildlife Pub Date : 2024-09-07 DOI:10.1016/j.ijppaw.2024.100985

Kana Kurusu , Naoyuki Hioki , Mizuho Shima , Sunao Kawakami , Yuta Hasebe , Noriyuki Takai , Jun Matsumoto , Aya Masuda

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Abstract

Myxobolus nagaraensis is a myxozoan parasite first reported in freshwater gobies (Rhinogobius spp.) from the Nagara River, Gifu Prefecture, Japan. Myxospores of M. nagaraensis form plasmodia in the visceral cavities of gobies, commonly presenting as distended abdomens. Although Rhinogobius is a common fish genus in Japan, details of M. nagaraensis, including genetic information, remain unknown. We compared the nucleotide sequences of the ribosomal RNA gene (rDNA) of M. nagaraensis from three different host species (R. fluviatilis, R. nagoyae, and R. similis) caught in three different rivers in Japan (Sakai, Sagami, and Kaname). The ITS region (ITS-1, 5.8S rDNA, and ITS-2) and large subunit (LSU) rDNA exhibited 49 and 55 variable sites, respectively. The highest nucleotide diversity was observed in the ITS region (0.00962), whereas that of the LSU rDNA was 0.00187. Differences in host species, rather than rivers, were a significant factor for genetic variation in both the ITS region (62.58%; P < 0.001) and LSU rDNA (55.22%; P < 0.01). Significant genetic variation was observed in M. nagaraensis from R. similis compared to R. fluviatilis (P < 0.001) or R. nagoyae (P < 0.001) from the same river. Such details are valuable for understanding parasite dispersal and its ecological impact on Rhinogobius hosts.

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日本河流中感染淡水虾虎鱼 Rhinogobius Gill 1859（戈壁形目：牛筋鱼科）的长尾糠虾（双壳纲：糠虾科）的遗传变异性

Nagara Myxobolus 是一种寄生于日本岐阜县长良川淡水虾虎鱼（Rhinogobius spp.）体内的肌孢子虫。长良蕈蚊的肌孢子在虾虎鱼的内脏腔中形成质体，通常表现为腹部膨胀。虽然Rhinogobius是日本常见的鱼属，但M. nagaraensis的详细信息（包括遗传信息）仍不为人所知。我们比较了在日本三条不同河流（堺、相模和神龟）中捕获的三种不同宿主物种（R. fluviatilis、R. nagoyae 和 R. similis）的长沼鲇核糖体 RNA 基因（rDNA）的核苷酸序列。ITS 区域（ITS-1、5.8S rDNA 和 ITS-2）和大亚基（LSU）rDNA 分别有 49 和 55 个可变位点。ITS 区域的核苷酸多样性最高（0.00962），而 LSU rDNA 的核苷酸多样性为 0.00187。宿主物种而非河流的差异是 ITS 区域（62.58%；P <；0.001）和 LSU rDNA（55.22%；P <；0.01）遗传变异的重要因素。与来自同一条河流的 R. fluviatilis（P <；0.001）或 R. nagoyae（P <；0.001）相比，在来自 R. similis 的 M. nagaraensis 中观察到了显著的遗传变异。这些细节对于了解寄生虫的传播及其对犀牛宿主的生态影响很有价值。

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来源期刊

International Journal for Parasitology-Parasites and Wildlife Medicine-Infectious Diseases

CiteScore

3.80

自引率

5.60%

发文量

113

审稿时长

45 days

期刊介绍： The International Journal for Parasitology: Parasites and Wildlife (IJP-PAW) publishes the results of original research on parasites of all wildlife, invertebrate and vertebrate. This includes free-ranging, wild populations, as well as captive wildlife, semi-domesticated species (e.g. reindeer) and farmed populations of recently domesticated or wild-captured species (e.g. cultured fishes). Articles on all aspects of wildlife parasitology are welcomed including taxonomy, biodiversity and distribution, ecology and epidemiology, population biology and host-parasite relationships. The impact of parasites on the health and conservation of wildlife is seen as an important area covered by the journal especially the potential role of environmental factors, for example climate. Also important to the journal is ''one health'' and the nature of interactions between wildlife, people and domestic animals, including disease emergence and zoonoses.