Enhancing the antibacterial activity of ampicillin loaded into chitosan/starch nanocomposites against AMR Staphylococcus aureus

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Carbohydrate Research Pub Date : 2024-09-13 DOI:10.1016/j.carres.2024.109274

Vinh Nghi Nguyen , Van Bon Nguyen , Minh Dinh Tran , Manh Dung Doan , Dinh Sy Nguyen , Thi Huyen Nguyen , Chien Thang Doan , Thi Ngoc Tran , San Lang Wang , Anh Dzung Nguyen

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Abstract

Ampicillin (Amp), an antibiotic, is widely used to treat bacterial infections in humans and livestock, but recently the rate of resistance has increased rapidly. The aim of this work was to enhancing the antibacterial effect of this compound against AMR Staphylococcus aureus via loading Amp into chitosan/starch nanocomposites by spray drying technique. The results showed that the different ratio of chitosan gel and starch gel used in preparing the nanocomposites can affect its properties and performance. The size distribution of the nanocomposite particles was ranging from 122.0 to 816.9 nm. The zeta potential values of the nanocomposites range from +29.47 to +93.07 mV, indicating the stability of the particles and their tendency to repel each other. Ampicillin was loaded into the chitosan/starch nanocomposites with encapsulation efficiency of 70.7–77.3 %, then their releasing and antibacterial effect against AMR S. aureus were investigated. The results indicated that antibacterial activity of chitosan/starch nanocomposites loaded ampicillin was much higher than ampicillin alone. Chitosan/starch nanocomposites loaded ampicillin at concentration 5.0 μg/mL inhibited 88.6 % growth of S. aureus to a similar extent as 7.5 μg/mL of ampicillin alone. Additionally, at same 7.5 μg/mL ampicillin concentration, the nanocomposites loaded ampicillin showed a higher inhibitory rate (93.27 %) compared to ampicillin alone (88.96 %) over a 12 h-period. Especially, the antibacterial activity of chitosan/starch nanocomposites loaded ampicillin still maintained their effectiveness over 48 h (95.43 %) while those the ampicillin decreased down to 85.76 %. This research highlights the potential of using the chitosan/starch nanocomposites as nanocarriers for ampicillin to enhance its antibacterial activity against AMR Staphylococcus aureus. This approach could be a promising strategy to combat antimicrobial resistance.

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增强壳聚糖/淀粉纳米复合材料负载的氨苄西林对金黄色葡萄球菌AMR的抗菌活性

氨苄西林（Amp）是一种抗生素，被广泛用于治疗人类和牲畜的细菌感染，但近来耐药率迅速上升。本研究的目的是通过喷雾干燥技术在壳聚糖/淀粉纳米复合材料中添加氨苄西林，增强该化合物对金黄色葡萄球菌的抗菌效果。结果表明，制备纳米复合材料时壳聚糖凝胶和淀粉凝胶的不同比例会影响其性质和性能。纳米复合材料颗粒的尺寸分布范围为 122.0 至 816.9 nm。纳米复合材料的 zeta 电位值在 +29.47 至 +93.07 mV 之间，表明颗粒的稳定性及其相互排斥的倾向。将氨苄西林负载到壳聚糖/淀粉纳米复合材料中，其封装效率为 70.7%-77.3% ，然后研究了它们对 AMR 金黄色葡萄球菌的释放和抗菌效果。结果表明，壳聚糖/淀粉纳米复合材料负载氨苄西林的抗菌活性远远高于单独负载氨苄西林的抗菌活性。浓度为 5.0 μg/mL 的壳聚糖/淀粉纳米复合材料负载氨苄西林对金黄色葡萄球菌生长的抑制率为 88.6%，与单用 7.5 μg/mL 氨苄西林的抑制率相近。此外，在相同的 7.5 μg/mL 氨苄西林浓度下，纳米复合材料负载的氨苄西林在 12 小时内的抑制率（93.27%）高于单独使用的氨苄西林（88.96%）。特别是，负载氨苄西林的壳聚糖/淀粉纳米复合材料的抗菌活性在 48 小时内仍然保持有效（95.43%），而负载氨苄西林的纳米复合材料的抗菌活性则下降到 85.76%。这项研究强调了使用壳聚糖/淀粉纳米复合材料作为氨苄西林的纳米载体来增强其对 AMR 金黄色葡萄球菌的抗菌活性的潜力。这种方法可能是一种很有前途的抗菌策略。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".