Effect of polyphenolic dendrimers on biological and artificial lipid membranes

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Chemistry and Physics of Lipids Pub Date : 2024-09-10 DOI:10.1016/j.chemphyslip.2024.105444

Marika Grodzicka , Sylwia Michlewska , Adam Buczkowski , Paula Ortega , Francisco Javier de la Mata , Maria Bryszewska , Maksim Ionov

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Abstract

The use of dendrimers as nanovectors for nucleic acids or drugs requires the understanding of their interaction with biological membranes. This study investigates the impact of 1st generation polyphenolic carbosilane dendrimers on biological and model lipid membranes using several biophysical methods. While the increase in the z-average size of DMPC/DPPG liposomes correlated with the number of caffeic acid residues included in the dendrimer structure, dendrimers that contained polyethylene glycol chains generated lower zeta potential when interacting with a liposomal membrane. The increase in the fluorescence anisotropy of DPH and TMA-DPH probes incorporated into erythrocyte membranes predicted the ability of dendrimers to affect membrane fluidity in the hydrophobic interior and hydrophilic/polar region of a lipid bilayer. The presence of caffeic acid and polyethylene glycol chains in the dendrimer structure affected the thermodynamical properties of the membrane lipid matrix.

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多酚树枝状聚合物对生物膜和人工脂膜的影响

将树枝状聚合物用作核酸或药物的纳米载体需要了解它们与生物膜的相互作用。本研究采用多种生物物理方法研究了第一代多酚碳硅烷树枝状聚合物对生物膜和模型脂膜的影响。DMPC/DPPG 脂质体 z 平均尺寸的增加与树枝状聚合物结构中含有的咖啡酸残基数量有关，而含有聚乙二醇链的树枝状聚合物在与脂质体膜相互作用时会产生较低的 zeta 电位。加入红细胞膜的 DPH 和 TMA-DPH 探针的荧光各向异性增加，这说明树枝状聚合物能够影响脂质双分子层疏水内部和亲水/极性区域的膜流动性。树枝状聚合物结构中的咖啡酸链和聚乙二醇链会影响膜脂基质的热力学性质。

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来源期刊

Chemistry and Physics of Lipids 生物-生化与分子生物学

CiteScore

7.60

自引率

2.90%

发文量

审稿时长

40 days

期刊介绍： Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications. Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.