Extraction and Physicochemical Characterization of Hydroxyapatites From Horse Humerus Bones of Different Ages (1, 3, 6, and 8 Years old) Calcined at Low Temperature

IF 3.2 4区 医学 Q2 ENGINEERING, BIOMEDICAL Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-09-18 DOI:10.1002/jbm.b.35484
Brandon A. Correa-Piña, Angelica M. Castillo-Paz, Urso Davila, Mario E. Rodriguez-Garcia
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Abstract

The aim of this work is to investigate the changes in the physicochemical properties of hydroxyapatite (HAp) extracted from horse humerus bones of different ages (1, 3, 6, and 8 years) subjected to low temperature calcination (600°C). Thermal analysis revealed significant mass loss due to water, collagen, organic compounds, carbonates, and age-related magnesium out-diffusion. Higher fat content in older bones contributed to increased mass loss. Phosphorus content remained constant across age groups, while calcium and sodium showed age-related fluctuations. Magnesium levels decreased with age, emphasizing its importance for early bone development. The Ca/P ratio deviated from the stoichiometric values due to additional ions from biogenic sources. Infrared spectroscopy identified functional groups in carbonated HAp, with changes observed before and after calcination. The full width at half maximum (FWHM) of the 961 cm−1 band decreased with age, indicating improved crystalline quality. The molar absorption coefficients provided information on the changes in molecular concentration and emphasized the differences between the age groups. X-ray analysis revealed nanocrystalline HAp in all samples, with crystallite size increasing with age. Rietveld analysis showed that the lattice parameters were affected by the presence of organic material, but the lattice constants remained stable, confirming high crystallinity independent of age. TEM analysis confirmed nanocrystalline structures, with crystallite size increasing with age. SEM images showed the characteristic porosity of calcined HAp, with particle size correlating positively with age. Calcination at 600°C preserved the nanoscale properties and microcrystal formation. Raman spectroscopy confirmed the identity of HAp, with FWHM variations indicating age-related changes in crystalline quality. EHAp1 showed increased FWHM, indicating lower crystalline quality and increased trace element content.

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低温煅烧不同年龄(1、3、6 和 8 岁)马肱骨中羟基磷灰石的提取和物理化学特征。
这项工作旨在研究从不同年龄(1、3、6 和 8 年)的马肱骨中提取的羟基磷灰石(HAp)在低温煅烧(600°C)后的理化性质变化。热分析表明,由于水分、胶原蛋白、有机化合物、碳酸盐和与年龄相关的镁外扩散,质量损失很大。年龄较大的骨骼中脂肪含量较高,导致质量损失增加。不同年龄组的磷含量保持不变,而钙和钠的含量则随着年龄的增长而波动。镁的含量随着年龄的增长而下降,强调了镁对早期骨骼发育的重要性。由于来自生物源的额外离子,钙/磷比率偏离了化学计量值。红外光谱确定了碳化 HAp 中的功能基团,并观察到煅烧前后的变化。961 cm-1 波段的半最大值全宽(FWHM)随着时间的推移而减小,表明结晶质量有所提高。摩尔吸收系数提供了分子浓度变化的信息,并强调了不同年龄组之间的差异。X 射线分析表明,所有样品中的 HAp 都是纳米结晶,结晶尺寸随年龄的增长而增大。里特维尔德分析表明,晶格参数受有机材料的影响,但晶格常数保持稳定,证实了高结晶度与年龄无关。TEM 分析证实了纳米结晶结构,结晶尺寸随着时间的推移而增大。扫描电子显微镜图像显示了煅烧后的 HAp 所特有的多孔性,颗粒大小与煅烧时间呈正相关。600°C 煅烧保留了纳米级特性和微晶形成。拉曼光谱证实了 HAp 的特性,其 FWHM 变化表明结晶质量发生了与年龄相关的变化。EHAp1 的 FWHM 增加,表明结晶质量降低,微量元素含量增加。
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来源期刊
CiteScore
7.50
自引率
2.90%
发文量
199
审稿时长
12 months
期刊介绍: Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.
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