Molecular characterization of anopheline species diversity in the Andaman and Nicobar archipelago, with a particular emphasis on Anopheles barbirostris

IF 1.8 3区 医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2024-09-17 DOI:10.1007/s00436-024-08348-1
Vidhya Thiruvoth Pachalil, Bhavna Gupta, Anwesh Maile, Ittoop Pulikkottil Sunish
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Abstract

This study investigates anopheline species diversity in the Andaman and Nicobar Islands, employing morphological and molecular methods, focusing on the D3 domain of 28S rRNA (D3) and second internal spacer (ITS2). Ten Anopheline species were identified morphologically and confirmed with molecular markers. While the D3 region demonstrated low level of inter- and intra-specific genetic distance in all the species, ITS2 revealed clear barcoding gap. Among the ten species, A. barbirostris exhibited significant diversity when compared with the sequences from other countries available in GenBank. Further analyses of additional samples of A. barbirostris were carried out using ITS2 and cytochrome oxidase I (COI) markers. Limited variations among the sequences from the islands were observed, suggesting a prevalent single molecular form. However, when compared with the GenBank sequences, our samples formed a separate cluster closely related to the A3 species. The genetic distance between our samples and the A3 cluster was 0.02 for COI but very high (0.104) for ITS2, suggesting a potentially new molecular form or species in the island region. This warrants a more comprehensive and detailed analysis of A. barbirostris in these islands at both genetic and morphometric levels. Overall, these observations added-up the new knowledge in the understanding of anopheline diversity in the Andaman and Nicobar archipelago and highlight the necessity for continuous molecular investigations to unravel complexities within mosquito population dynamics.

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安达曼和尼科巴群岛疟原虫物种多样性的分子特征,特别强调巴比罗斯特疟原虫
本研究采用形态学和分子方法调查了安达曼和尼科巴群岛的疟原虫物种多样性,重点研究了 28S rRNA 的 D3 结构域(D3)和第二内部间隔(ITS2)。通过形态学方法鉴定了 10 个疟原虫物种,并用分子标记进行了确认。所有物种的 D3 区域都显示出较低的种间和种内遗传距离,而 ITS2 则显示出明显的条形码差距。在这 10 个物种中,与 GenBank 中其他国家的序列相比,A. barbirostris 表现出显著的多样性。利用 ITS2 和细胞色素氧化酶 I(COI)标记对更多的 A. barbirostris 样本进行了进一步分析。观察到各岛屿的序列之间差异有限,表明普遍存在单一分子形式。然而,与 GenBank 序列相比,我们的样本形成了一个独立的群组,与 A3 种密切相关。我们的样本与 A3 群的 COI 遗传距离为 0.02,但 ITS2 的遗传距离却非常高(0.104),这表明该岛屿地区可能存在一种新的分子形式或物种。这就需要对这些岛屿上的 A. barbirostris 在遗传和形态水平上进行更全面、更详细的分析。总之,这些观察结果为了解安达曼和尼科巴群岛的按蚊多样性提供了新的知识,并强调了持续进行分子研究以揭示蚊子种群动态复杂性的必要性。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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