Spectroscopic Response of Chiral Proteophenes Binding to Two Chiral Insulin Amyloids

IF 3 4区 化学 Q3 CHEMISTRY, PHYSICAL ChemPhotoChem Pub Date : 2024-09-20 DOI:10.1002/cptc.202400225
Takahiro Watanabe, Priyanka Swaminathan, Linnea Björk, Ayaka Nakanishi, Hisako Sato, Tamotsu Zako, K. Peter R. Nilsson, Mikael Lindgren
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Abstract

We recently reported on 8 different pentameric oligothiophenes, denoted proteophenes, with different amino acid substitution patterns along the conjugated thiophene backbone. The proteophenes were forming chiral both molecular (solvent dissolved) and self-assembled nano-architectonic structures, the latter having approximately 5-6 times greater ICD responses. Herein, two proteophenes, HS-84-E-E and HS-84-K-K, were further investigated in terms of binding to two chiral variants of insulin amyloid fibrils. Binding curves based on fluorescence revealed strong primary binding sites for both proteophenes and more unspecific secondary binding for especially the latter. Interestingly, their induced circular dichroism (ICD) responses were similar to or stronger than for their solvent form and showed a complicated alteration upon binding suggesting that the microstructure at the binding site governs the helical structure of the ligand. Hyperspectral fluorescence microscopy and FLIM revealed more details on the molecular binding in terms of proteophene emission decay-times. Vibrational circular dichroism (VCD) analysis showed that VCD signal of amyloid fibrils was enhanced upon binding of proteophenes, suggesting changes in the amyloid microstructures.
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手性蛋白酶与两种手性胰岛素淀粉样蛋白结合的光谱响应
我们最近报告了 8 种不同的五聚体低聚噻吩,称为蛋白噻吩,其共轭噻吩骨架具有不同的氨基酸取代模式。这些蛋白噻吩形成了手性分子结构(溶于溶剂)和自组装纳米结构,后者的 ICD 反应大约是前者的 5-6 倍。在此,我们进一步研究了两种蛋白酚(HS-84-E-E 和 HS-84-K-K)与两种手性胰岛素淀粉样纤维变体的结合情况。基于荧光的结合曲线显示,这两种蛋白酚都有很强的主要结合位点,尤其是后者的非特异性次要结合位点较多。有趣的是,它们的诱导圆二色性(ICD)反应与其溶剂形式相似或更强,并且在结合时显示出复杂的变化,这表明结合位点的微观结构控制着配体的螺旋结构。高光谱荧光显微镜和 FLIM 从蛋白吩发射衰减时间的角度揭示了分子结合的更多细节。振动圆二色性分析表明,蛋白酚结合后,淀粉样纤维的振动圆二色性信号增强,表明淀粉样微结构发生了变化。
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来源期刊
ChemPhotoChem
ChemPhotoChem Chemistry-Physical and Theoretical Chemistry
CiteScore
5.80
自引率
5.40%
发文量
165
期刊最新文献
Front Cover: Diindeno-Fused Corannulene-Extended Tetrathiafulvalenes (ChemPhotoChem 10/2024) Spectroscopic Response of Chiral Proteophenes Binding to Two Chiral Insulin Amyloids Novel Photobase Generators Derived from Proazaphosphatrane–Aryl Borate for High-Pressure Mercury Lamp Lithography Modulating N–H Bond Cleavage in Catalytic Ammonia Oxidation Reaction Design and Synthesis of Aggregation-Caused Quenching Hydroxy-Phenanthroimidazole Derivatives for Probing of Fe3+ ions and as Potential Blue Light Emitters
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