Multi-parametric Photoacoustic Imaging Combined with Acoustic Radiation Force Impulse Imaging for Applications in Tissue Engineering

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL Annals of Biomedical Engineering Pub Date : 2024-09-18 DOI:10.1007/s10439-024-03617-7
Christopher D. Nguyen, Ying Chen, David L. Kaplan, Srivalleesha Mallidi
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Abstract

Tissue engineering is a dynamic field focusing on the creation of advanced scaffolds for tissue and organ regeneration. These scaffolds are customized to their specific applications and are often designed to be complex, large structures to mimic tissues and organs. This study addresses the critical challenge of effectively characterizing these thick, optically opaque scaffolds that traditional imaging methods fail to fully image due to their optical limitations. We introduce a novel multi-modal imaging approach combining ultrasound, photoacoustic, and acoustic radiation force impulse imaging. This combination leverages its acoustic-based detection to overcome the limitations posed by optical imaging techniques. Ultrasound imaging is employed to monitor the scaffold structure, photoacoustic imaging is employed to monitor cell proliferation, and acoustic radiation force impulse imaging is employed to evaluate the homogeneity of scaffold stiffness. We applied this integrated imaging system to analyze melanoma cell growth within silk fibroin protein scaffolds with varying pore sizes and therefore stiffness over different cell incubation periods. Among various materials, silk fibroin was chosen for its unique combination of features including biocompatibility, tunable mechanical properties, and structural porosity which supports extensive cell proliferation. The results provide a detailed mesoscale view of the scaffolds’ internal structure, including cell penetration depth and biomechanical properties. Our findings demonstrate that the developed multimodal imaging technique offers comprehensive insights into the physical and biological dynamics of tissue-engineered scaffolds. As the field of tissue engineering continues to advance, the importance of non-ionizing and non-invasive imaging systems becomes increasingly evident, and by facilitating a deeper understanding and better characterization of scaffold architectures, such imaging systems are pivotal in driving the success of future tissue-engineering solutions.

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多参数光声成像结合声辐射力脉冲成像在组织工程中的应用
组织工程是一个充满活力的领域,其重点是为组织和器官再生创造先进的支架。这些支架是根据其具体应用定制的,通常设计成复杂的大型结构,以模拟组织和器官。传统成像方法由于其光学局限性而无法对这些厚而光学不透明的支架进行全面成像,本研究解决了有效表征这些支架的关键挑战。我们介绍了一种结合超声波、光声学和声辐射力脉冲成像的新型多模态成像方法。这种组合利用声学检测技术克服了光学成像技术的局限性。超声成像用于监测支架结构,光声成像用于监测细胞增殖,声辐射力脉冲成像用于评估支架硬度的均匀性。我们应用这种集成成像系统分析了黑色素瘤细胞在不同孔径的丝纤维蛋白支架中的生长情况,因此支架的硬度也随细胞培养时间的不同而变化。在各种材料中,我们选择了蚕丝纤维蛋白,因为它具有独特的综合特性,包括生物相容性、可调机械特性和结构孔隙率,这些特性支持细胞的广泛增殖。研究结果提供了支架内部结构的详细中尺度视图,包括细胞渗透深度和生物力学特性。我们的研究结果表明,所开发的多模态成像技术能全面揭示组织工程支架的物理和生物动态。随着组织工程领域的不断发展,非电离、非侵入式成像系统的重要性日益凸显,通过促进对支架结构更深入的了解和更好的表征,此类成像系统在推动未来组织工程解决方案取得成功方面起着举足轻重的作用。
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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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