The structural role of Skp1 in the synaptonemal complex is conserved in nematodes.

IF 3.3 3区生物学 Genetics Pub Date : 2024-09-18 DOI:10.1093/genetics/iyae153

Lisa E Kursel,Kaan Goktepe,Ofer Rog

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Abstract

The synaptonemal complex (SC) is a meiotic interface that assembles between parental chromosomes and is essential for gamete formation. While the dimensions and ultrastructure of the SC are conserved across eukaryotes, its protein components are highly divergent. Recently, an unexpected component of the SC has been described in the nematode C. elegans: the Skp1-related proteins SKR-1/2, which are components of the Skp1, Cullin, F-box (SCF) ubiquitin ligase. Here, we find that the role of SKR-1 in the SC is conserved in P. pacificus. The P. pacificus Skp1 ortholog, Ppa-SKR-1, colocalizes with other SC proteins throughout meiotic prophase, where it occupies the middle of the SC. Like in C. elegans, the dimerization interface of Ppa-SKR-1 is required for its SC function. A dimerization mutant, ppa-skr-1F105E, fails to assemble SC and produces almost no progeny. Interestingly, the evolutionary trajectory of SKR-1 contrasts with other SC proteins. Unlike most SC proteins, SKR-1 is highly conserved in nematodes. Our results suggest that the structural role of SKR-1 in the SC has been conserved since the common ancestor of C. elegans and P. pacificus, and that rapidly evolving SC proteins have maintained the ability to interact with SKR-1 for at least 100 million years.

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Skp1在突触神经复合体中的结构作用在线虫中是保守的。

突触复合体（SC）是在亲本染色体之间组装的减数分裂界面，对配子的形成至关重要。虽然SC的尺寸和超微结构在真核生物中是保守的，但其蛋白质成分却有很大差异。最近，在线虫秀丽隐杆线虫（C. elegans）中发现了一种意想不到的 SC 组成成分：Skp1 相关蛋白 SKR-1/2，它们是 Skp1、Cullin、F-box（SCF）泛素连接酶的组成成分。在这里，我们发现 SKR-1 在 SC 中的作用在太平洋鼠中是保守的。太平洋鼠的 Skp1 同源物 Ppa-SKR-1 在整个减数分裂前期与其他 SC 蛋白共定位，占据 SC 的中间位置。与优雅子一样，Ppa-SKR-1 的二聚化界面也是其 SC 功能所必需的。二聚化突变体 ppa-skr-1F105E 无法组装 SC，几乎不产生后代。有趣的是，SKR-1 的进化轨迹与其他 SC 蛋白形成了鲜明对比。与大多数 SC 蛋白不同，SKR-1 在线虫中高度保守。我们的研究结果表明，自线虫和太平洋鼠的共同祖先以来，SKR-1在SC中的结构作用一直保持不变，而且快速进化的SC蛋白至少在一亿年中一直保持着与SKR-1相互作用的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genetics 生物-遗传学

CiteScore

6.20

自引率

6.10%

发文量

177

期刊介绍： GENETICS is published by the Genetics Society of America, a scholarly society that seeks to deepen our understanding of the living world by advancing our understanding of genetics. Since 1916, GENETICS has published high-quality, original research presenting novel findings bearing on genetics and genomics. The journal publishes empirical studies of organisms ranging from microbes to humans, as well as theoretical work. While it has an illustrious history, GENETICS has changed along with the communities it serves: it is not your mentor''s journal. The editors make decisions quickly – in around 30 days – without sacrificing the excellence and scholarship for which the journal has long been known. GENETICS is a peer reviewed, peer-edited journal, with an international reach and increasing visibility and impact. All editorial decisions are made through collaboration of at least two editors who are practicing scientists. GENETICS is constantly innovating: expanded types of content include Reviews, Commentary (current issues of interest to geneticists), Perspectives (historical), Primers (to introduce primary literature into the classroom), Toolbox Reviews, plus YeastBook, FlyBook, and WormBook (coming spring 2016). For particularly time-sensitive results, we publish Communications. As part of our mission to serve our communities, we''ve published thematic collections, including Genomic Selection, Multiparental Populations, Mouse Collaborative Cross, and the Genetics of Sex.