Molecular imprinting based sensor system developed using polymeric nanoparticles for detecting 17β‐estradiol in agricultural wastewater

IF 2.7 3区 化学 Q2 POLYMER SCIENCE Journal of Applied Polymer Science Pub Date : 2024-09-17 DOI:10.1002/app.56276
Esra Yaşar, Hilal Özçelik, Timuçin Güner, Emre Dokuzparmak, Sinan Akgöl
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Abstract

Micro pollutants pose a significant issue in water ecosystems. Particularly high concentrations of 17β‐estradiol (E2) have been identified in agricultural wastewater, which poses harmful effects on aquatic organisms and disrupts ecosystem balance. Therefore, effective determination of E2 from water sources is crucial. This study developed a biosensor capable of detecting E2 in wastewater using specific polymer nanoparticle synthesis through molecular imprinting. Est‐imp‐poly(multi‐walled carbon nanotubes‐glycidyl methacrylate [MWCNT‐GMA]) polymer nanoparticles were synthesized using a surfactant‐free emulsion polymerization method, and their characterization was conducted using FTIR and scanning electron microscopy (SEM) technologies. The Qmax value for Est‐imp‐poly(MWCNT‐GMA) nanoparticles in a 1 mg/mL E2 solution was determined to be 140 ppm. Comparing adsorption capacities, the molecularly imprinted nanoparticles (MIP) showed nearly five times higher E2 adsorption compared to non‐imprinted polymers (NIP). The Est‐imp‐poly(MWCNT‐GMA)‐Nafion/screen‐printed electrode (SPE) system was employed for analyzing wastewater samples. The current measurements taken at various concentrations in the wastewater consistently matched the E2 concentration calibration curve. The limit of detection (LoD) and limit of quantification (LoQ) were determined to be 0.042 and 0.12 μM, respectively. The biosensor demonstrated a linear working range from 0.12 to 50 μM, with a high correlation coefficient (R2 = 0.9927). These results highlight the potential of the developed biosensor for detecting E2 in real samples.
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利用聚合物纳米颗粒开发的基于分子印迹技术的传感器系统,用于检测农业废水中的 17β-estradiol
微污染物是水生态系统中的一个重要问题。特别是在农业废水中发现了高浓度的 17β-雌二醇(E2),这对水生生物造成了有害影响,并破坏了生态系统平衡。因此,有效测定水源中的 E2 至关重要。本研究利用分子印迹技术合成了特异性聚合物纳米粒子,开发了一种能够检测废水中 E2 的生物传感器。采用无表面活性剂乳液聚合法合成了 Est-imp-poly (多壁碳纳米管-甲基丙烯酸缩水甘油酯 [MWCNT-GMA])聚合物纳米粒子,并利用傅立叶变换红外光谱(FTIR)和扫描电子显微镜(SEM)技术对其进行了表征。在 1 mg/mL E2 溶液中,Est-imp-poly(MWCNT-GMA) 纳米粒子的 Qmax 值被测定为 140 ppm。比较吸附能力,分子印迹纳米粒子(MIP)对 E2 的吸附能力是非印迹聚合物(NIP)的近五倍。利用 Est-imp-聚(MWCNT-GMA)-Nafion/丝网印刷电极(SPE)系统分析废水样品。废水中不同浓度的电流测量值与 E2 浓度校准曲线一致。检测限(LoD)和定量限(LoQ)分别为 0.042 和 0.12 μM。该生物传感器的线性工作范围为 0.12 至 50 μM,相关系数很高(R2 = 0.9927)。这些结果凸显了所开发的生物传感器在检测实际样品中 E2 的潜力。
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来源期刊
Journal of Applied Polymer Science
Journal of Applied Polymer Science 化学-高分子科学
CiteScore
5.70
自引率
10.00%
发文量
1280
审稿时长
2.7 months
期刊介绍: The Journal of Applied Polymer Science is the largest peer-reviewed publication in polymers, #3 by total citations, and features results with real-world impact on membranes, polysaccharides, and much more.
期刊最新文献
Editorial Board, Aims & Scope, Table of Contents Editorial Board, Aims & Scope, Table of Contents Editorial Board, Aims & Scope, Table of Contents Editorial Board, Aims & Scope, Table of Contents Cover Image, Volume 141, Issue 43
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